Regenerated plant in-vitro culture method for Sirindhorn michelia figo
A technology for in vitro culture and regeneration of plants, applied in the field of biological in vitro tissue culture seedlings, can solve the problems of hindering the development of reproductive technology, browning, high pollution rate, etc., achieve high induction rate and survival rate, low transplant survival rate, precious, The effect is simple and feasible
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[0033] Example 1 The following steps are used to realize the in vitro culture and regeneration of Sirindhorn Smiley:
[0034] (1) Treatment of explants and their materials: The young shoots sprouted from the main pole of the 7-year-old Sirindhorn Michelangelo tree, which grows vigorously and are disease-free, are used in the Shenzhou Mulan Garden, Xuwen County, Guangdong Province. , Bring it back to the laboratory at low temperature; cut off the leaves on the twigs in the laboratory, wash with neogermide, rinse with running water, and cut into 2cm stem segments with 1-2 axillary buds;
[0035] (2) Establish aseptic propagules: sterilize the trimmed stems with 0.1% mercuric chloride in the laboratory for 3 minutes, then wash with sterile water 4 times, and inoculate one bottle of one segment on the primary medium MS1 , Cultured for 15 days under the conditions of room temperature 30℃, daily light for 8 hours and light intensity of 2000LX, green shoots sprouted from the leaf axils, a...
Example Embodiment
[0045] Example 2 The following steps are used to realize the in vitro culture and regeneration of the Sirindhorn smile:
[0046] (1) Treatment of explants and their materials: The young shoots sprouted from the main pole of the 7-year-old Sirindhorn Michelangelo tree, which grows vigorously and are disease-free, are used in the Shenzhou Mulan Garden, Xuwen County, Guangdong Province. , Bring it back to the laboratory at low temperature; cut off the leaves on the twigs in the laboratory, wash with neogermide, rinse with running water, and cut into 2cm stem segments with 1-2 axillary buds;
[0047] (2) Establish aseptic propagules: sterilize the trimmed stems in the laboratory with 0.1% mercuric chloride for 10 minutes, then wash with sterile water 3 times, and inoculate one bottle of one segment on the primary medium MS1 , Cultured for 20 days under the conditions of room temperature 20℃, daily light for 12 hours and light intensity 1000LX, green shoots sprouted from the leaf axils,...
Example Embodiment
[0057] Example 3 The following steps are used to realize the in vitro culture and regeneration of the Sirindhorn smile:
[0058] (1) Treatment of explants and their materials: The young shoots sprouted from the main pole of the 7-year-old Sirindhorn Michelangelo tree, which grows vigorously and are disease-free, are used in the Shenzhou Mulan Garden, Xuwen County, Guangdong Province. , Bring it back to the laboratory at low temperature; cut off the leaves on the twigs in the laboratory, wash with neogermide, rinse with running water, and cut into 2cm stem segments with 1-2 axillary buds;
[0059] (2) Establish aseptic propagules: sterilize the trimmed stems in the laboratory with 0.1% mercuric chloride for 5 minutes, then wash with sterile water 3 times, and inoculate one bottle of one segment on the primary medium MS1 , Cultured for 30 days under the conditions of room temperature 26℃, daily light for 10 hours and light intensity of 800LX, green shoots sprouted from the leaf axils...
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