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Regenerated plant in-vitro culture method for Sirindhorn michelia figo

A technology for in vitro culture and regeneration of plants, applied in the field of biological in vitro tissue culture seedlings, can solve the problems of hindering the development of reproductive technology, browning, high pollution rate, etc., achieve high induction rate and survival rate, low transplant survival rate, precious, The effect is simple and feasible

Inactive Publication Date: 2014-04-16
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the tissue culture propagation of Magnoliaceae plants has the problems of high pollution rate, serious browning, low induction rate, and low transplant survival rate. For the propagation of endangered tree species such as Sirindhorn Michelle, these problems hinder the development of its reproductive technology. bottleneck

Method used

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  • Regenerated plant in-vitro culture method for Sirindhorn michelia figo
  • Regenerated plant in-vitro culture method for Sirindhorn michelia figo
  • Regenerated plant in-vitro culture method for Sirindhorn michelia figo

Examples

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Effect test

Embodiment 1

[0033] Example 1 The following steps are used to achieve the in vitro culture regeneration of Sirindhorn Michelle:

[0034] (1) Treatment of explants and their materials: In the Shenzhou Mulan Garden in Xuwen County, Guangdong Province, the young shoots that germinated on the main pole of the 7-year-old Sirindhorn Michelle, which is healthy and free of diseases and insect pests, were taken after on-site fresh-keeping treatment , brought back to the laboratory at low temperature; cut off the leaves on the tender shoots in the laboratory, rinse with running water after cleaning with bromogeramine, and cut into 2 cm stem segments with 1-2 axillary buds;

[0035] (2) Establish sterile propagules: sterilize the pruned stems in the laboratory with 0.1% mercuric chloride for 3 minutes, then wash 4 times with sterile water, and inoculate a section of a bottle on the primary medium MS1 , cultured for 15 days at room temperature of 30°C, 8 hours of light per day, and light intensity of ...

Embodiment 2

[0045] Example 2 The following steps are used to realize the regenerated plants of Sirindhorn Michelle in vitro:

[0046] (1) Treatment of explants and their materials: In the Shenzhou Mulan Garden in Xuwen County, Guangdong Province, the young shoots that germinated on the main pole of the 7-year-old Sirindhorn Michelle, which is healthy and free of diseases and insect pests, were taken after on-site fresh-keeping treatment , brought back to the laboratory at low temperature; cut off the leaves on the tender shoots in the laboratory, rinse with running water after cleaning with bromogeramine, and cut into 2 cm stem segments with 1-2 axillary buds;

[0047] (2) Establish sterile propagules: sterilize the pruned stems in the laboratory with 0.1% mercuric chloride for 10 minutes, then wash with sterile water for 3 times, and inoculate a section of a bottle on the primary medium MS1 , cultured for 20 days at room temperature of 20°C, 12 hours of light per day, and light intensity...

Embodiment 3

[0057] Embodiment 3 adopts the following steps to realize the in vitro culture regeneration plant of Sirindhorn Michelle:

[0058] (1) Treatment of explants and their materials: In the Shenzhou Mulan Garden in Xuwen County, Guangdong Province, the young shoots that germinated on the main pole of the 7-year-old Sirindhorn Michelle, which is healthy and free of diseases and insect pests, were taken after on-site fresh-keeping treatment , brought back to the laboratory at low temperature; cut off the leaves on the tender shoots in the laboratory, rinse with running water after cleaning with bromogeramine, and cut into 2 cm stem segments with 1-2 axillary buds;

[0059] (2) To establish sterile propagules: sterilize the pruned stem segments with 0.1% mercuric chloride for 5 minutes in the laboratory, then wash with sterile water for 3 times, and inoculate a section of a bottle on the primary medium MS1 , cultured for 30 days at room temperature of 26°C, 10 hours of light per day, ...

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Abstract

The invention discloses a regenerated plant in-vitro culture method for Sirindhorn michelia figo. The regenerated plant in-vitro culture method comprises the following six steps: (1) adopting explants and treating materials; (2) establishing a sterile propagule; (3) carrying out multiplication culture; (4) carrying out rooting culture; (5) transplanting; and (6) culturing seedlings. According to the regenerated plant in-vitro culture method for the Sirindhorn michelia figo, the inductivity and the survival rate are high; the steps are simple, convenient and feasible; the regenerated plant in-vitro culture method has very urgent and important meanings on preserving and rescuing world valuable and rare endangered michelia figo varieties and breeding extremely-endangered trees; the regenerated plant in-vitro culture method is used for carrying out tissue culture on big tree twigs of michelia figo plants; the problems of a tissue culture propagation technology that the pollution rate is low, the browning is serious, the inductivity is low, the transplanting survival rate is low and the like are overcome and the method has the very valuable academic research value.

Description

technical field [0001] The invention relates to a method for in vitro culture and regeneration of plants, in particular to a method for in vitro culture and regeneration of Sirindhorn Michelle, and belongs to the technical field of biological in vitro tissue culture and seedling cultivation. Background technique [0002] Sirindhorniae (Scientific name: Michelia sirindhorniae (Noot. et Chal.) N. H. Xia et X. H. Zhangj) At present, Magnolia sirindhorniae Noot. & Chalermglin is widely used in the world. , is a critically endangered tree species, native to Thailand, is an evergreen tree, leaves and flowers are very aromatic, can extract valuable plant essence. [0003] Sirindhorn Michelia is a large evergreen tree of Magnoliaceae, with a straight trunk and a height of 30-35 m. The flowers are ivory white. A single flower can bloom for 2-3 days, and the flowering period is in June-July. Sirindhorn was born in the high-humidity freshwater swamp forest. It is the only tree species...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨小红黄少伟莫晓勇朱开甫董君
Owner SOUTH CHINA AGRI UNIV
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