Regenerated plant in-vitro culture method for Sirindhorn michelia figo

A technology for in vitro culture and regeneration of plants, applied in the field of biological in vitro tissue culture seedlings, can solve the problems of hindering the development of reproductive technology, browning, high pollution rate, etc., achieve high induction rate and survival rate, low transplant survival rate, precious, The effect is simple and feasible

Inactive Publication Date: 2014-04-16
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the tissue culture propagation of Magnoliaceae plants has the problems of high pollution rate, serious browning, low induction rate, and low transplant

Method used

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  • Regenerated plant in-vitro culture method for Sirindhorn michelia figo
  • Regenerated plant in-vitro culture method for Sirindhorn michelia figo
  • Regenerated plant in-vitro culture method for Sirindhorn michelia figo

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1 The following steps are used to realize the in vitro culture and regeneration of Sirindhorn Smiley:

[0034] (1) Treatment of explants and their materials: The young shoots sprouted from the main pole of the 7-year-old Sirindhorn Michelangelo tree, which grows vigorously and are disease-free, are used in the Shenzhou Mulan Garden, Xuwen County, Guangdong Province. , Bring it back to the laboratory at low temperature; cut off the leaves on the twigs in the laboratory, wash with neogermide, rinse with running water, and cut into 2cm stem segments with 1-2 axillary buds;

[0035] (2) Establish aseptic propagules: sterilize the trimmed stems with 0.1% mercuric chloride in the laboratory for 3 minutes, then wash with sterile water 4 times, and inoculate one bottle of one segment on the primary medium MS1 , Cultured for 15 days under the conditions of room temperature 30℃, daily light for 8 hours and light intensity of 2000LX, green shoots sprouted from the leaf axils, a...

Example Embodiment

[0045] Example 2 The following steps are used to realize the in vitro culture and regeneration of the Sirindhorn smile:

[0046] (1) Treatment of explants and their materials: The young shoots sprouted from the main pole of the 7-year-old Sirindhorn Michelangelo tree, which grows vigorously and are disease-free, are used in the Shenzhou Mulan Garden, Xuwen County, Guangdong Province. , Bring it back to the laboratory at low temperature; cut off the leaves on the twigs in the laboratory, wash with neogermide, rinse with running water, and cut into 2cm stem segments with 1-2 axillary buds;

[0047] (2) Establish aseptic propagules: sterilize the trimmed stems in the laboratory with 0.1% mercuric chloride for 10 minutes, then wash with sterile water 3 times, and inoculate one bottle of one segment on the primary medium MS1 , Cultured for 20 days under the conditions of room temperature 20℃, daily light for 12 hours and light intensity 1000LX, green shoots sprouted from the leaf axils,...

Example Embodiment

[0057] Example 3 The following steps are used to realize the in vitro culture and regeneration of the Sirindhorn smile:

[0058] (1) Treatment of explants and their materials: The young shoots sprouted from the main pole of the 7-year-old Sirindhorn Michelangelo tree, which grows vigorously and are disease-free, are used in the Shenzhou Mulan Garden, Xuwen County, Guangdong Province. , Bring it back to the laboratory at low temperature; cut off the leaves on the twigs in the laboratory, wash with neogermide, rinse with running water, and cut into 2cm stem segments with 1-2 axillary buds;

[0059] (2) Establish aseptic propagules: sterilize the trimmed stems in the laboratory with 0.1% mercuric chloride for 5 minutes, then wash with sterile water 3 times, and inoculate one bottle of one segment on the primary medium MS1 , Cultured for 30 days under the conditions of room temperature 26℃, daily light for 10 hours and light intensity of 800LX, green shoots sprouted from the leaf axils...

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Abstract

The invention discloses a regenerated plant in-vitro culture method for Sirindhorn michelia figo. The regenerated plant in-vitro culture method comprises the following six steps: (1) adopting explants and treating materials; (2) establishing a sterile propagule; (3) carrying out multiplication culture; (4) carrying out rooting culture; (5) transplanting; and (6) culturing seedlings. According to the regenerated plant in-vitro culture method for the Sirindhorn michelia figo, the inductivity and the survival rate are high; the steps are simple, convenient and feasible; the regenerated plant in-vitro culture method has very urgent and important meanings on preserving and rescuing world valuable and rare endangered michelia figo varieties and breeding extremely-endangered trees; the regenerated plant in-vitro culture method is used for carrying out tissue culture on big tree twigs of michelia figo plants; the problems of a tissue culture propagation technology that the pollution rate is low, the browning is serious, the inductivity is low, the transplanting survival rate is low and the like are overcome and the method has the very valuable academic research value.

Description

technical field [0001] The invention relates to a method for in vitro culture and regeneration of plants, in particular to a method for in vitro culture and regeneration of Sirindhorn Michelle, and belongs to the technical field of biological in vitro tissue culture and seedling cultivation. Background technique [0002] Sirindhorniae (Scientific name: Michelia sirindhorniae (Noot. et Chal.) N. H. Xia et X. H. Zhangj) At present, Magnolia sirindhorniae Noot. & Chalermglin is widely used in the world. , is a critically endangered tree species, native to Thailand, is an evergreen tree, leaves and flowers are very aromatic, can extract valuable plant essence. [0003] Sirindhorn Michelia is a large evergreen tree of Magnoliaceae, with a straight trunk and a height of 30-35 m. The flowers are ivory white. A single flower can bloom for 2-3 days, and the flowering period is in June-July. Sirindhorn was born in the high-humidity freshwater swamp forest. It is the only tree species...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨小红黄少伟莫晓勇朱开甫董君
Owner SOUTH CHINA AGRI UNIV
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