A method for rapidly cultivating oil-containing microorganisms

Abstract

The invention discloses a rapid cultivation method of oleaginous microorganisms. The method includes the steps of: (1) cultivating an oleaginous yeast seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the oleaginous yeast seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, culturing the oleaginous yeast in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture condition are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the oleaginous yeast cells in the cultivation process. The photobioreactor also comprises a guide shell, the membrane separation device is arranged in the guide shell, and an outlet of the guide shell is leveling with the outlet of the photobioreactor; and the photobioreactor comprises a reflective membrane pasted on the outer surface of the guide shell. The method has the advantages of increasing the yield of autotrophic microalgae and oleaginous yeast cells, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating oleaginous microorganisms, in particular to a method for semi-mixed culture of autotrophic microalgae and oleaginous yeast to obtain high yields of oleaginous microbial cells and simultaneously increase the oil content of oleaginous microbial cells . Background technique

[0002] With the development of society and economy, the supply of fossil resources is becoming increasingly scarce, the contradiction between supply and demand of oil products is becoming more and more prominent, and the corresponding environmental pollution problems are also becoming more prominent. It is inevitable to develop renewable resources through multiple channels. Biodiesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, s...

Examples

Embodiment Construction

[0023] The method for quickly cultivating oil-containing microorganisms of the present invention specifically includes the following content: before the semi-mixed culture, two kinds of oil-containing microorganisms are separately cultured in seed liquid, a stirring bioreactor is used for oil-containing yeast seed liquid culture, and a photobioreactor is used for self-cultivation. For microalgae seed liquid cultivation, the stirred bioreactor includes oleaginous yeast and oleaginous yeast fermentation medium, and the photobioreactor includes autotrophic microalgae and autotrophic SE medium. After the seed solution is qualified, it is transferred to the built-in membrane separation device for cultivating facultative anaerobic bacteria and the closed photobioreactor with reflective film for cultivating autotrophic microalgae through their respective inoculation lines, and added to the The nutrient components and acid-base neutralizer of the mixed culture medium treated by the bac...

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a method for cultivating oleaginous microorganisms, in particular to a method for semi-mixed culture of autotrophic microalgae and oleaginous yeast to obtain high yields of oleaginous microbial cells and simultaneously increase the oil content of oleaginous microbial cells . Background technique

[0002] With the development of society and economy, the supply of fossil resources is becoming increasingly scarce, the contradiction between supply and demand of oil products is becoming more and more prominent, and the corresponding environmental pollution problems are also becoming more prominent. It is inevitable to develop renewable resources through multiple channels. Biodiesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, s...

Examples

Embodiment Construction

[0023] The method for quickly cultivating oil-containing microorganisms of the present invention specifically includes the following content: before the semi-mixed culture, two kinds of oil-containing microorganisms are separately cultured in seed liquid, a stirring bioreactor is used for oil-containing yeast seed liquid culture, and a photobioreactor is used for self-cultivation. For microalgae seed liquid cultivation, the stirred bioreactor includes oleaginous yeast and oleaginous yeast fermentation medium, and the photobioreactor includes autotrophic microalgae and autotrophic SE medium. After the seed solution is qualified, it is transferred to the built-in membrane separation device for cultivating facultative anaerobic bacteria and the closed photobioreactor with reflective film for cultivating autotrophic microalgae through their respective inoculation lines, and added to the The nutrient components and acid-base neutralizer of the mixed culture medium treated by the bac...

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