Method for detecting polyaspartic acid in polypeptide urea by high-performance liquid chromatography
A technology of high performance liquid chromatography and polyaspartic acid, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of reliable detection data
Inactive Publication Date: 2015-06-10
XINJIANG UYGUR AUTONOMOUS REGION PROD QUALITY SUPERVISION & INSPECTION RES INST
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[0003] The content of polyaspartic acid in polypeptide urea directly affects the quality and application performance of the product, so it is very important to analyze the content of polyaspartic acid in polypeptide urea, and there is no HPLC analysis method for polyaspartic acid content in polypeptide urea report
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[0019] The method for detecting polyaspartic acid in polypeptide urea by high performance liquid chromatography, the high performance liquid chromatography condition in this method is:
[0020] Chromatographic column: C18, 5μm, 250×4.6mm (I.D.);
[0021] Column temperature: room temperature;
[0022] Mobile phase composition: volume ratio methanol: water = 6:94, water is double distilled water, add 0.04% volume percentage triethylamine;
[0023] Mobile phase pH: adjusted to pH9.5 with dilute phosphoric acid 1:10;
[0024] Mobile phase velocity: flow rate 0.7ml / min;
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Abstract
The invention relates to a method for detecting polyaspartic acid in polypeptide urea by high-performance liquid chromatography. Reversed-phase high-performance liquid chromatography is adopted for measuring the content of polyaspartic acid in polypeptide urea, and reliable detection data is provided for controlling the contents of urea and polyaspartic acid in polypeptide urea. The content of polyaspartic acid in polypeptide urea can be detected through the simple method, and a detection result is accurate and stable.
Description
technical field [0001] The invention relates to a method for detecting polyaspartic acid in polypeptide urea by high performance liquid chromatography. Background technique [0002] Polypeptide urea is a new type of fertilizer that has appeared on the market in recent years, also known as magnetic polypeptide urea. Its essence is to add polyaspartic acid (or polyaspartic acid) to traditional urea, and it is a new type of fertilizer made through some other processing techniques. Polyaspartic acid is a biomimetic synthetic water-soluble polymer substance, which has the characteristics of phosphorus-free, non-toxic, pollution-free and completely biodegradable. It is an internationally recognized "green chemical". Due to the structural characteristics of polyaspartic acid containing active groups such as peptide bonds and carboxyl groups, it has extremely strong chelating, dispersing, and adsorption functions, and has excellent compatibility. Polyaspartic acid can prolong the ...
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IPC IPC(8): G01N30/89
Inventor 杨博冉文生李小飞姚海军葛春辉
Owner XINJIANG UYGUR AUTONOMOUS REGION PROD QUALITY SUPERVISION & INSPECTION RES INST
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