Rabies vaccine glycoprotein content detection method

A detection method and rabies vaccine technology, applied in the biological field, can solve the problems such as no reports, no public method for detecting rabies virus antigen content, etc.

Active Publication Date: 2014-05-07
LIAONING CHENGDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no domestic method for detecting the content of rabies virus antigen, and no relevant reports from foreign companies can be found.

Method used

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  • Rabies vaccine glycoprotein content detection method
  • Rabies vaccine glycoprotein content detection method
  • Rabies vaccine glycoprotein content detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The detection method of this rabies vaccine glycoprotein content has the following steps:

[0016] 1. Prepare a suitable dilution of human anti-rabies virus immunoglobulin as a coating solution. Add 200 μl of coating solution to each well, seal with parafilm, place in a humid chamber at 37°C for 3 hours, and then overnight at 4°C. Take out the empty plate, add 300 μl / well blocking solution, seal it with a parafilm and place it in a wet box at 37°C for 1 hour, finally empty the plate, seal it with a parafilm, and store it at -70°C.

[0017] 2. Dilute each sample to several dilutions by the 2-fold method (the dilution can be increased when the glycoprotein content is high). Reference vaccine: 8 dilutions by 2-fold method. Take the pre-coated ELISA plate out of the -70°C refrigerator, wash it 4 times with a plate washer, 300 μl / well of washing solution, and empty the plate. Add 200 μl / well of sample, add diluent in the first column as a blank, and no sample; add referen...

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Abstract

The invention relates to a rabies vaccine glycoprotein content detection method which is characterized by using a sandwich ELISA (enzyme-linked immuno sorbent assay) method for rabies virus antigen content detection, and the rabies vaccine glycoprotein content detection method is as follows: firstly, coating a 96-hole elisa plate with rabies virus polyclonal antibody, adding a to-be-tested sample for incubation at 37 DEG C for 1.5 hours; cleaning the elisa plate for 5 times; then adding the mouse anti-rabies virus monoclonal antibody for incubation at 37 DEG C for 1.5 hours; cleaning the elisa plate for 5 times, adding an anti-mouse enzyme conjugate for incubation at 37 DEG C for 1 hour; cleaning the elisa plate for 6 times, adding a substrate for coloration; using a microplate reader for detecting, reading an OD (optical density) value; and calculating the rabies virus glycoprotein content. The rabies vaccine glycoprotein content detection method can be used for quality control of rabies vaccine industry, and can quickly identify the authenticity of rabies vaccine.

Description

technical field [0001] The invention relates to a detection method of biological preparations, in particular to a detection method for the glycoprotein content of rabies vaccine which is used for quality control in the rabies vaccine industry and can quickly identify the authenticity of the rabies vaccine, and belongs to the field of biotechnology. Background technique [0002] Rabies virus has a strong tropism for nerve tissue, and rabies is a fatal infectious disease. Rabies vaccine plays an important role in preventing rabies. Rabies virus glycoprotein is the most important component of rabies virus surface antigen and is the main immune antigen of rabies virus. Its content and activity play a vital role in vaccine quality. Detecting the glycoprotein content of rabies virus plays an indirect role in evaluating vaccine efficacy, which is a beneficial supplement to vaccine efficacy experiments. Compared with efficacy experiments, it saves more time, reduces the use of ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/54366G01N33/68
Inventor 高旭哲战辛刘畅
Owner LIAONING CHENGDA BIOTECH
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