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Method for selective breeding of rice thermo-sensitive permanent genic male sterile line by adopting bridge parents

A technology for bridging parental and sterile lines, which is applied in the field of new material innovation in the utilization of rice heterosis, can solve the problems of easy drift of fertility, poor adaptability, and reduced seed purity, so as to solve the problem of purity and production risks, The effect of solving the narrow selection range and solving the tense seed production season

Inactive Publication Date: 2014-05-28
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the two-line hybrid rice has further increased the yield per unit area of ​​rice in my country, there are also varieties in production that are not well adapted to abnormal climate conditions such as high temperature and cold damage, and show poor yield stability. More importantly, the temperature-sensitive GMS line , under special climatic conditions, the fertility is prone to drift, resulting in self-crossing phenomenon, which affects the safe seed production of two-line hybrid rice, resulting in reduced seed purity and reduced agricultural production

Method used

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  • Method for selective breeding of rice thermo-sensitive permanent genic male sterile line by adopting bridge parents
  • Method for selective breeding of rice thermo-sensitive permanent genic male sterile line by adopting bridge parents
  • Method for selective breeding of rice thermo-sensitive permanent genic male sterile line by adopting bridge parents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Breeding process of photothermosensitive genic male sterile and inverse thermosensitive genic male sterile near-isogenic lines

[0047] 1.1 Screening of germplasm materials

[0048] 1.1.1 Parental materials: Guangmo S, 2301S, 1892S, Guangzhan 63S, P88S, Aizi S (the temperature-sensitive karyophyte produced by crossing Pei'ai 64S and Zihui 100 in the Rice Research Institute of Anhui Academy of Agricultural Sciences) 16 temperature-sensitive genic male sterile lines, including Xin'an S / / Xin'an s / 2301S from the Rice Research Institute of Anhui Academy of Agricultural Sciences), Pei'ai 64S, and Aizhan 43S; Yannong s, Dwarf goose s and other 4 anti-thermosensitive GMS lines.

[0049] 1.1.2 Sowing: In December 2006 in Hainan, it was sown in three phases: 1st, 10th, and 20th. The seedlings were transplanted at a age of 30 days, and the spacing between plants and rows was 4×5 inches.

[0050] 1.1.3 Hybrid breeding: A single plant of the anti-thermo-sensitive genic ...

Embodiment 2

[0056] Example 2: Fertility Screening of Thermosensitive GMS Lines under Natural Conditions

[0057] 2.1 Sampling: From the beginning of ear to the full ear stage, in the seed selection nursery, select a single plant with a relatively short plant height, strong resistance, and excellent economic properties, select a single ear that has just begun to ear, and take the upper and middle branches of the ear and send it indoors Microscopic examination.

[0058] 2.2 Microscopic examination: take the anthers of the middle and upper florets, and take out all 6 anthers in each floret, or take 6 anthers of 6 florets, put them on a glass slide, drop an appropriate amount of 1% I-KI solution, and use After the anthers were fully crushed and stained with tweezers, the pollen types were observed under a 10×10 magnification microscope. It is usually divided into four types: aborted, dyed, rounded and normal. Only normal pollen can be self-fertilized, and completely aborted plants have no po...

Embodiment 3

[0060] Example 3: Fertility Observation of Anti-TGMS Lines under Natural Conditions

[0061] 3.1 Test method: planted under natural conditions in Hainan, sowed on December 15, seedling age 30 days, planted 4×5+10 inches.

[0062] 3.2 Fertility observation: From the beginning of earing to the end of earing, observe in the field every 3-5 days, check the size and color of anthers, and finally check the seed setting rate. Individual plants that show no fruit under different temperature conditions may be Anti-thermosensitive genic male sterile individual plants can be considered for election.

[0063] 3.3 Precautions: First, to avoid the wrong selection of physiologically sterile plants, this requires frequent observation and elimination; second, to prevent the wrong selection of mixed strains, such materials may be sterile for life; third, pay attention to the influence of diseases and phytotoxicity, so as to avoid False anti-therm GMS lines were selected.

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Abstract

The invention discloses a method for selective breeding of a rice thermo-sensitive permanent genic male sterile line by adopting bridge parents. With utilization of a hybridization technology, recessive genic male sterile genes for expressing sterility under conditions of high temperature and low temperature are respectively introduced into appropriate bridge parents; through hybrid backcross and trait screening, a pair of near-isogenic lines having bridge parent genetic backgrounds and respectively carried with a high temperature genic male sterile trait and a low temperature genic male sterile trait are bred; the rice permanent genic male sterile line is produced through outcrossing breeding of the pair of near-isogenic lines, so that production risks brought because the two kinds of thermo-sensitive genic male sterile lines are affected by the environment in the production process of two-line hybrid rice seeds are avoided, a two-line hybrid rice seed production system which can randomly select a seed production base and a seed production season is established, and a new way for utilization of rice heterosis is developed.

Description

technical field [0001] The invention belongs to the innovative field of new material utilization of rice heterosis, and specifically relates to a method and application of rice temperature-sensitive permanent genetic male sterile line breeding by using bridge parents. Background technique [0002] The two-line hybrid rice is a major scientific research achievement of my country's independent innovation in agricultural science and technology. Its successful development, promotion and application have solved the long-term problems of the three-line hybrid rice, such as the limited recovery spectrum, single cytoplasm, and complex breeding and seed production procedures. A new course in the development of hybrid rice. However, while the two-line hybrid rice has further improved the yield per unit area of ​​rice in my country, there are also varieties in the production that are not well adapted to abnormal climate conditions such as high temperature and cold damage, and are charac...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02
Inventor 王德正吴爽杜士云王辉许学
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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