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Preparation method for extracted antradia camphorata fermented whole-liquid active substances for resisting avian influenza virus

A technology of avian influenza virus and active substances, which is applied in the field of extraction of active substances from the whole liquid of Antrodia camphorata anti-avian influenza virus, to achieve the effects of increasing production, solving the difficulty of artificial cultivation, and high yield

Inactive Publication Date: 2014-05-28
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Regarding the preparation method for the extraction of active substances from the fermented whole liquid of Antrodia camphorata against avian influenza virus, the relevant patents retrieved include: Chinese patent application number 99112556.8 Grifola frondosa Extraction method of polysaccharide from fermentation broth, 02136517.2 Fermentation process of Grifola frondosa and production method of its polysaccharide peptide, 89105471 Production method of polysaccharide peptide (PSP) from versicolor, 92109345 Extraction process of lentinan and lentinan, 96109095 Chinese Ganoderma lucidum health drink, 92111030 Ganoderma lucidum nutrition and health care liquid and its preparation method, 93103116 Ganoderma lucidum oral liquid and other patents. Most of these patents are mainly for the extraction of polysaccharides. Among them, the three active substance extracts prepared from Grifola frondosa, Yunzhi and Ganoderma lucidum have anti-tumor, anti-inflammatory and anti-inflammatory properties. High blood pressure, blood sugar lowering, anti-obesity, anti-hepatitis and other medicinal effects, but there are few reports on anti-viral effects, and there are no reports on anti-avian influenza virus.

Method used

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  • Preparation method for extracted antradia camphorata fermented whole-liquid active substances for resisting avian influenza virus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation method for extracting active substance from whole liquid of Antrodia camphorata anti-avian influenza virus

[0050] 1. Fermentation medium formula:

[0051] 2. Potato starch 1%, maltose 1%, peptone 0.2%, yeast extract 0.2%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.05%.

[0052] 2 liquid seed culture:

[0053]A 500ml Erlenmeyer flask is used, and each bottle is filled with 200ml of liquid medium. The fermentation culture method is to inoculate 10% Antrodia camphorata strains into a Erlenmeyer flask filled with liquid medium in a conventional way, adjust the pH to 6, cultivate at 28°C, shake the flask at a rotational speed of 150r / min, and vibrate for 15 sky. Antrodia camphorata liquid seeds can be obtained.

[0054] 3. Fermentation tank fermentation:

[0055] A 50L fermenter is used for the liquid fermentation of Antrodia camphorata. The sterilization pressure of the fermenter is 0.1-0.2 kg / cm2, and the sterilization time is 1.5 hours. T...

Embodiment 2

[0066] A preparation method for extracting active substances from the fermented whole liquid of Antrodia camphorata anti-avian influenza virus,

[0067] 1 Fermentation medium formula:

[0068] Potato starch 2%, maltose 3%, peptone 0.1%, yeast extract 0.3%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.05%.

[0069] 2 liquid seed culture:

[0070] A 500ml Erlenmeyer flask is used, and each bottle is filled with 200ml of liquid medium. The fermentation culture method is that 5% Antrodia camphorata strains are inoculated in a Erlenmeyer flask equipped with a liquid medium in a conventional method, pH? , cultivated under the condition of 30°C, the rotation speed of the shaker flask was 200r / min, and the culture was shaken for 10 days. Antrodia camphorata liquid seeds can be obtained.

[0071] 3. Fermentation tank fermentation:

[0072] A 50L fermenter is used for the liquid fermentation of Antrodia camphorata. The sterilization pressure of the fermenter is 0.1-0.2 ...

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Abstract

The invention discloses a preparation method for extracted antradia camphorata fermented whole-liquid active substances for resisting avian influenza virus. The method comprises the following step: antradiacomphora fermented whole liquid or fermented clear liquor or mycelium is subjected to heating, filtration, vacuum concentration, alcohol precipitation, centrifugal separation, collection drying, collection smashing and the like. The antradiacomphora mycelium fermented whole-liquid active substances extracted by the method has obvious inhibiting effect on the avian influenza virus; the method has the characteristics of simple process, high yield, low cost and good effect.

Description

(one) technical field [0001] The invention relates to a preparation method for extracting active substances from the fermented whole liquid of Antrodia camphorata against avian influenza virus, in particular to Antrodia camphorata ( Antrodia camphorata ) Ac001, which was deposited on September 21, 2005 in the "Common Microorganism Center of China Committee for the Collection of Microorganisms" with the preservation number CGMCC No.1460. (two) Background technique [0002] Regarding the preparation method for the extraction of active substances from the fermentation liquid of Antrodia camphorata against avian influenza virus, the relevant patents retrieved include: Chinese patent application number 99112556.8 Extraction method of polysaccharides from fermented liquor of Grifola frondosa, 02136517.2 Fermentation process of Grifola frondosa and its polysaccharides Peptide production method, 89105471 A production method of versicolor glycopeptide (PSP), 92109345 Lentinan a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61P31/16
Inventor 宋爱荣赵晨孔超黄芳孙效乐
Owner QINGDAO AGRI UNIV