Functional nano catechin gene-introduction material and preparation method thereof

Abstract

The invention discloses a functional nano catechin gene-introduction material. Catechin and ferrous sulfate are combined into a catechin-Fe<2+> nanometer spherical material of which the average diameter is about 100 nm, the catechin content is 0.94 g / g, and the ferrous ion content is 0.06 g / g. The invention further discloses a preparation method of the functional nano catechin gene-introduction material. The functional nano catechin gene-introduction material has relatively high transfection efficiency which can reach about 40 percent in human breast carcinoma cells. The toxicity is low, the catechin is high in biocompatibility, and thus the cell survival rate is very high. The dispersity of the material is high and the material conforms to the requirements of transfection; the cost is extremely low; the catechin is a main active ingredient extracted from tea leaves, namely a polyphenolic compound, the price of the catechin is low, and ferrous sulfate is also a common cheap reagent which is easy to get. For the material preparation, the reaction is simple and easy in operation, and the repeatability is high.

Description

[technical field] [0001] The invention relates to a high transfection efficiency and low cytotoxicity functionalized nano-catechin gene transfer material and a preparation method thereof. [Background technique] [0002] With the completion of the determination of the human gene map, our understanding of the pathogenesis of human diseases has made breakthrough progress at the genetic level. Current research shows that the occurrence and development of many diseases are closely related to genes. If the gene fragments or gene mutations specifically related to the disease can be screened out, specific treatments can be targeted at the gene level, such as by introducing related deletion genes or silencing genes to enhance related deletion functions or silence disease-causing genes , so as to achieve the purpose of complete treatment. Safely and effectively introducing target genes into organisms is the key and difficult point in this research field. [0003] Gene transfer syst...

AI Technical Summary

Problems solved by technology

Since there are many serious deficiencies in the viral gene transfer system, such as the possibility of activation of proto-oncogenes by viral transfection, the non-viral transfection method is currently a research hotspot. In the above-mentioned non-viral transfection, microinjection once Only one cell can be processed; the penetration of the gene gun is very limited; the results of the calcium phosphate co-precipitation method are unstable; only the cationic liposome method shows good transfection efficiency, but the high toxicity limits its application

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