Direct detection of chloramphenicol in honey by neutral desorption-electrospray extraction ionization mass spectrometry
A neutral desorption and ionized substance technology, applied in the field of detection, can solve the problems of cumbersome operation steps, time-consuming, inconvenient detection of chloramphenicol, etc., and achieves the effect of high desorption efficiency and improved strength
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[0032] 1. Treatment of spiked chloramphenicol honey: prepare 1.0mg / mL chloramphenicol standard solution with methanol to make 1.0mg / mL chloramphenicol standard solution for later use; accurately measure 1mL honey sample, add chloramphenicol standard solution Finally, dilute with 20mL of methanol: water (1:1) to make a chloramphenicol honey solution with a concentration gradient of 100-8000ng / mL, add a stirring bar and stir on the stirrer at room temperature for 3min, measure 7mL of the diluted honey solution To 10mL Erlenmeyer flask for experiment.
[0033] 2. Sample preparation: Take 1mL of the honey to be tested, add 20mL of methanol: water diluent with a volume ratio of 1:1 to dilute, take 7mL into a 10mL Erlenmeyer flask.
[0034] 3. Neutral desorption-electrospray extraction ionization mass spectrometry (ND-EESI-MS):
[0035] (1) Set the ND-EESI ion source to the negative ion mode, the mass range is 50-500Da, the neutral desorption spray reagent is methanol, the methanol...
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