Application of fulvic acid salt in preparation of medicament for promoting angiogenesis

A technology of fulvic acid and angiogenesis, which is applied in the field of fulvic acid, can solve the problem that the effect of promoting angiogenesis has not been reported yet, and achieve the effect of reducing economic burden, high safety and remarkable curative effect

Active Publication Date: 2014-06-04
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A large number of experimental studies and clinical curative effects at home and abroad have shown that it has significant effects such as promoting blo...

Method used

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  • Application of fulvic acid salt in preparation of medicament for promoting angiogenesis
  • Application of fulvic acid salt in preparation of medicament for promoting angiogenesis
  • Application of fulvic acid salt in preparation of medicament for promoting angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Effect of fulvic acid sodium / potassium salt on the proliferation of human umbilical vein endothelial cell lines (HUVECs) (MTT method)

[0019] This example studies the effect of fulvic acid sodium / potassium salt on the proliferation of human umbilical vein endothelial cell lines (HUVECs). The experimental method is as follows:

[0020] Human umbilical vein endothelial cell lines (HUVECs, purchased from ATCC, USA) were incubated at 37°C in 5% CO 2 cultured under conditions, and seeded into 96-well plates at a density of 3000 cells per well after growing to the logarithmic phase. After cultivating for another 24 hours under the above conditions, the sodium / potassium fulvic acid salts were formulated into solutions with a concentration of 50mg / L, 100mg / L, 200mg / L, 300mg / L, and 400mg / L, and added to the 96-well plate, and continued Cultivate for 48 h, add MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) 20 μL per well after 48 h, continue to cult...

Embodiment 2

[0028] Example 2: Experiment of promoting effect of fulvic acid sodium / potassium salt on angiogenesis of chicken embryo allantoic membrane

[0029] The present embodiment studies sodium fulvic acid / potassium salt to promote angiogenesis of chicken embryo allantoic membrane, and experimental method is as follows:

[0030] Take fresh eggs at a temperature of 37.8 °C, CO 2 The concentration was 5%, and incubated for 7 days under the condition of suitable humidity. Open a window (1×1 cm) at the end of the air chamber, use a sterile filter paper sheet with a diameter of 6 mm as the drug delivery carrier, place it on the allantoic membrane, and administer it at different concentrations of 100, 200, 400, and 600 mg / L Set up a blank group (normal saline group) and a positive control drug group (Beifuji), seal the window with scotch tape, and continue to incubate under the above conditions; after 72 hours, remove the allantoic membrane tissue and put it in After dehydration and fixa...

Embodiment 3

[0036] Embodiment 3: the preparation of sodium fulvate / potassium salt tablet

[0037] Prescription (1000 tablets): 50g sodium / potassium fulvic acid, 125g pregelatinized starch, 15g sodium carboxymethylcellulose, 50g microcrystalline cellulose, 30g Libeng, 114g white sugar powder, 6g magnesium stearate, Coating powder 10g.

[0038] Add sodium / potassium fulvic acid into pregelatinized starch, sodium carboxymethyl cellulose, microcrystalline cellulose, Libeng, and white sugar powder according to the prescription amount, mix well to make granules, add magnesium stearate, and mix well Coated after tableting, 1000 tablets (each tablet contains 0.125g of sodium / potassium fulvic acid salt).

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Abstract

The invention discloses application of a fulvic acid salt in preparation of a medicament for promoting angiogenesis. A clinical research shows that angiogenesis opens up a new therapy pathway for revascularization of ischemic angiopathy. An experiment result shows that the fulvic acid salt disclosed by the invention has a human umbilical vein endothelial cell proliferation promoting activity and a chick embryo chorioallantoic membrane angiogenesis promoting activity, and can be applied to promoting angiogenesis therapy of ischemic angiopathy (for example, a peripheral arterial ischemic vascular disease, thromboangiitis obliterans, a foot disease in diabetes and the like).

Description

technical field [0001] The invention relates to a new application of fulvic acid salt, in particular to the application of fulvic acid salt in the preparation of drugs for promoting angiogenesis. Background technique [0002] Ischemic vascular diseases such as peripheral arterial ischemic vascular disease, vaso-occlusive vasculitis, and diabetic foot disease have become major diseases that threaten human health. At present, the main treatment measures for it include routine blood circulation promoting and blood stasis removal, revascularization and so on. However, a considerable number of patients are difficult to respond to drug treatment, are not suitable for conventional revascularization, or have a poor prognosis. Therefore, new treatment methods must be developed. [0003] In recent years, promoting angiogenesis has become a new direction for the treatment of ischemic vascular diseases and is one of the current research hotspots. Angiogenesis mainly involves process...

Claims

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Application Information

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IPC IPC(8): A61K35/02A61P9/10
Inventor 何静杨静陈绍军李宝才丁亚芳王美玲
Owner KUNMING UNIV OF SCI & TECH
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