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A method for the determination of shikimic acid in Shengmai injection

A technique for Shengmai injection and a determination method, which is applied in the field of determination of shikimic acid in Shengmai injection, can solve the problems that quality control means cannot characterize biological substance components, and is difficult to control internal quality, and achieves good linearity and strong specificity. Effect

Active Publication Date: 2016-12-21
YAAN THREE NINE PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Because traditional Chinese medicine has a complex material basis, its quality control methods cannot characterize its complex biological material components, and it is actually difficult to control its intrinsic quality

Method used

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  • A method for the determination of shikimic acid in Shengmai injection
  • A method for the determination of shikimic acid in Shengmai injection
  • A method for the determination of shikimic acid in Shengmai injection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1 (preparation of Shengmai Injection)

[0072] [Prescription] Red Ginseng 100g Ophiopogon japonicus 312g Schisandra 156g

[0073] [Preparation method] The above three flavors and red ginseng are cut into thin slices or crushed, soaked in more than 90% ethanol and heated to reflux for extraction 4 to 5 times, each time for 2 hours; or continuous reflux extraction for 6.5 hours, combined extracts, refrigerated, filtered, Recover ethanol until each 1ml contains 0.3-0.4g of crude drug, add an appropriate amount of activated carbon, stir, adjust the pH value of the filtrate to near neutral, let it stand still, concentrate the filtrate to a thick paste, add water for injection to 200ml, stir well, refrigerate, filter, Add an appropriate amount of activated carbon to boil, filter, and the filtrate is used for liquid preparation; Schisandra chinensis, if necessary, crush, collect 150ml of distillate by steam distillation, refrigerate, for liquid preparation, add wate...

Embodiment 2

[0074] Embodiment 2 (determination of shikimic acid content)

[0075] a. Take a sample of Shengmai Injection and dilute it by 20 times as the test product;

[0076] b. Take the shikimic acid reference substance and prepare it to about 80 μg / ml;

[0077] c. Carry out high-performance liquid chromatography determination, wherein the chromatographic conditions are: chromatographic column: Phenomenex Synergi 4μmHydro-RP 80A 250×4.60mm, 4micron, detection wavelength: 218nm; column temperature: 30°C; flow rate: 0.6ml / mim; mobile phase : 20mM / L KH 2 PO 4 Solution (H 3 PO 4 adjust the pH to 2.5);

[0078] d. Determination of shikimic acid content in Shengmai injection by external standard method.

Embodiment 3

[0079] Embodiment 3 (determination of shikimic acid content)

[0080] a. Take a sample of Shengmai Injection and dilute it by 20 times as the test product;

[0081] b. Take the shikimic acid reference substance and prepare it to about 80 μg / ml;

[0082] c. Carry out high-performance liquid chromatography determination, wherein the chromatographic conditions are: chromatographic column: Phenomenex Synergi 4μmHydro-RP 80A 250×4.60mm, 4micron, detection wavelength: 218nm; column temperature: 30°C; flow rate: 0.6ml / mim; mobile phase : 15mM / L KH 2 PO 4 Solution (H 3 PO 4 adjust the pH to 2.5);

[0083] d. Determination of shikimic acid content in Shengmai injection by external standard method.

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Abstract

The invention relates to a method for determining shikimic acid of a pulse-activating injection. The method comprises the steps: injecting the pulse-activating inspection in a high performance liquid chromatograph after properly diluting, and determining the content of the shikimic acid by adopting an external standard method.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, in particular to a method for determining shikimic acid in Shengmai injection. Background technique [0002] Shengmai Injection is a traditional Chinese medicine injection for intravenous injection developed according to the famous classic traditional Chinese medicine "Shengmaisan". [0003] The ancient prescription "Shengmaisan" has been used for thousands of years, and it is safe, effective and enduring. Ancient doctors not only used it to rescue severe diseases such as heat-induced vitality, body fluid consumption, and weak pulse, but also used it as a tonic for patients with deficiency of both qi and yin. Regarding the meaning of the prescription name of Shengmaisan, Wu Kun said in "Medicine Prescription Research": "The person named Shengmai is full of energy when the pulse is obtained, and weak when the energy is lost, so it is named." The pulse of traditional Chinese medicine ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 赵炳祥朱雅宁董礼王虎山侯新莲吴建国祝勇军
Owner YAAN THREE NINE PHARMA