Gene transfer material and preparation method of functionalized nano curcumin
A technology of gene introduction and curcumin, applied in the direction of introducing foreign genetic material using a vector, recombinant DNA technology, etc., can solve the problems of limited penetration of the gene gun and unstable results, and achieve good biocompatibility and material dispersibility. Good, low-cost results
Inactive Publication Date: 2018-08-03
王深明 +2
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Problems solved by technology
Since there are many serious deficiencies in the viral gene transfer system, such as the possibility of activation of proto-oncogenes by viral transfection, the non-viral transfection method is currently a research hotspot. In the above-mentioned non-viral transfection, microinjection once Only one cell can be processed; the penetration of the gene gun is very limited; the results of the calcium phosphate co-precipitation method are unstable; only the cationic liposome method shows good transfection efficiency, but the high toxicity limits its application
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[0022] 1. Preparation of the gene transfer material of the present invention:
[0023] 1. Take materials: main materials: zinc chloride, analytically pure, product of Aldrich Company; curcumin, MW660, analytically pure, used without further purification steps, product of Aldrich Company. All preparative glassware were sonicated in ethanol for 5 minutes, then rinsed with double distilled water, and washed with washing solution H 2 0 / HNO 3 (65%) / H 2 o 2 (35%) (1:1:1, v / v / v) for cleaning, followed by double-distilled water and acetone for cleaning, and finally air-dried.
[0024] 2. Preparation of materials: Dissolve zinc chloride in deionized water to prepare 0.1 mmol / L, and dissolve curcumin in absolute alcohol to prepare 2 mmol / L fresh curcumin solution. First, add 1ml of 2mmol / L fresh curcumin solution to 0.1mmol / L zinc chloride solution, place it in a 25mL beaker, stir thoroughly for 30min, let it stand for 1 day, centrifuge at 8000r / m speed, and then use double distille...
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Abstract
The invention discloses a functionalized nano-curcumin gene introduction material, which is a composite material composed of curcumin and zinc chloride, with an average diameter of about 100nm, a curcumin content of 97.77wt%, and a zinc ion content of 2.23wt%. , forming curcumin-Zn2+nano-spherical material. The invention also discloses a preparation method of the functionalized nano-curcumin gene transfer material. The gene introduction material of the present invention has higher transfection efficiency, which can reach about 40% in human breast cancer cells; low toxicity, curcumin has good biocompatibility, and the cell survival rate is very high; the material dispersibility is good, The method meets the requirements for transfection; the cost is very low; the material preparation reaction is simple and easy to operate, and the repeatability is good; the application prospect is good, and it can be applied to the production of antitumor drugs.
Description
[technical field] [0001] The invention relates to a high transfection efficiency and low cytotoxicity functionalized nano-curcumin gene introduction material and a preparation method. [Background technique] [0002] With the completion of the determination of the human gene map, our understanding of the pathogenesis of human diseases has made breakthrough progress at the genetic level. Current research shows that the occurrence and development of many diseases are closely related to genes. If the gene fragments or gene mutations specifically related to the disease can be screened out, specific treatments can be targeted at the gene level, such as by introducing related deletion genes or silencing genes to enhance related deletion functions or silence disease-causing genes , so as to achieve the purpose of complete treatment. Safely and effectively introducing target genes into organisms is the key and difficult point in this research field. [0003] Gene transfer systems ...
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IPC IPC(8): C12N15/85
Inventor 王深明张德元刘勇夏浩明周鸿雁李梓伦常光其徐安武
Owner 王深明