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A method for shortening the rooting time of test-tube plantlets of long handle almond

A technology of almond with long handle and test tube seedling, applied in the biological field, can solve problems such as difficult to achieve satisfactory results, and achieve the effects of improving rooting efficiency, shortening rooting period, and shortening rooting time

Inactive Publication Date: 2015-09-30
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, it is difficult to achieve satisfactory results with conventional auxin-induced methods alone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Acquisition of adventitious buds of almond

[0025] Take the stem section of the long-stalked almonds test tube plantlet with a node of about 3 cm, sterilize the surface with a sodium hypochlorite solution or a mercuric chloride solution, and then receive 0.5 mg / L of indole butyric acid and 0.2 mg / L of 6-benzyl Cultivate on MS solid medium of aminoadenine, the culture temperature is 25℃±2℃, the light intensity is 4200Lux, and the photoperiod is 16 hours; a large number of adventitious buds will be produced after 40 days of culture;

[0026] 2. Treatment of adventitious buds of almonds with long handle

[0027] When adventitious shoots elongated to 3 cm, they were transferred to MS solid medium supplemented with 0.2 mg / L indolebutyric acid to induce root formation. Visible roots grew after 30 days; after 45 days, the average root number of the test-tube seedlings was 2.3 and the root length was 4.6 cm, which could be used for transplanting and domestication.

Embodiment 2

[0029] 1. Acquisition of adventitious buds of almond

[0030] Same as Example 1.

[0031] 2. Treatment of adventitious buds of almonds with long handle

[0032] When adventitious shoots elongated to 3 cm, they were transferred to MS solid medium supplemented with 0.2 mg / L indolebutyric acid to induce root formation. Among them, KNO in MS medium 3 , NH 4 NO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O and CaCl2 2H 2 O as a whole adjusts its dose to 1 / 4 of the original. Visible roots grew after 25 days; after 35 days, the average root number of the test-tube seedlings was 3.4 and the root length was 5.0 cm, which could be used for transplanting and domestication.

Embodiment 3

[0034] 1. Acquisition of adventitious buds of almond

[0035] Same as Example 1.

[0036] 2. Treatment of adventitious buds of almonds with long handle

[0037] When adventitious shoots elongated to 3 cm, they were transferred to MS solid medium supplemented with 0.2 mg / L indolebutyric acid to induce root formation. Among them, KNO in MS medium 3 , NH 4 NO 3 、KH 2 PO 4 , MgSO 4 7H 2 O and CaCl 2 2H 2 O as a whole adjusts its dose to 1 / 8 of the original. Visible roots grew after 20 days; after 30 days, the average root number of the test-tube seedlings was 3.3 and the root length was 6.2 cm, which could be used for transplanting and domestication.

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Abstract

The invention discloses a method for shortening the rooting time of amygdalus pedunculata pall test-tube plantlets. Specific to obtained amygdalus pedunculata pall adventitious buds, the dosage of nutrient elements in a conventional culture medium is adjusted, and a proper amount of lanthanum nitrate is added, so that the rooting period of amygdalus pedunculata pall test-tube plantlets is shortened remarkably, and meanwhile, the root numbers and the root lengths of test-tube plantlets are increased. The method is simple and feasible and can be used for rapidly propagating amygdalus pedunculata pall, and the rooting efficiency of the amygdalus pedunculata pall test-tube plantlets is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for shortening the rooting time of test-tube seedlings of Amygdalus pedunculata Pall. Background technique [0002] Long handle almond (Amygdalus pedunculata Pall.) is a deciduous shrub of the Rosaceae (Rosaceae) peach genus (Amygdalus) subgenus (Amygdalus), also known as wild cherry, handle almond, hair cherry, mainly distributed in Inner Mongolia and Shaanxi and other regions , also distributed in Siberia and Mongolia. Almonds have important economic and ecological value. Long handle almond kernel contains crude fat 531g / kg, crude protein 255.9g / kg, iron 39.88mg / kg, zinc 42.16mg / kg, calcium 0.224mg / kg, high nutritional value. The fruit contains the laxative ingredient Amygdaluside (3-rhamnoglucosylkaempferol), which can be widely used in medical and health care. It has certain curative effects on bronchitis, high blood pressure, neurasthenia, pneumonia, and d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭斌尉亚辉何玮郭丽珠
Owner NORTHWEST UNIV
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