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A method for simultaneously extracting icariin and baohuoside I and II from epimedium

A technology of icariin and epimedium, applied in the field of simultaneous extraction of icariin and baohuoside I, II, which can solve the problems of small environmental pollution, short production cycle, and low extraction purity, and achieve low environmental pollution , short production cycle, good thermal stability

Inactive Publication Date: 2015-11-18
成都合盛生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for simultaneously extracting Epimedium from Epimedium with high yield, short production cycle, low energy consumption and little environmental pollution. The method of Huoside and Baohuoside I, II

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After crushing the Epimedium plant, pass it through a 40-mesh sieve, add purified water 6 times the total weight of the Epimedium plant, heat and reflux for extraction twice, each time for 2 hours, and filter to obtain filtrate A; Add 13 times the total weight of Epimedium plants in an ethanol solution with a content of 60%, heat and reflux for extraction twice, extract for 1 hour each time, and obtain filtrate B by filtration; mix filtrate A and filtrate B, perform suction filtration, concentrate and recover under reduced pressure Ethanol to obtain the primary extract; the obtained primary extract is extracted with purified water, adjusted to PH2, left to stand, and suction filtered to obtain the icariin extract; the macroporous adsorption resin is used to wash the above-mentioned icariin extract Remove, elute the macroporous adsorption resin with ethanol solution, and collect and retain the eluate; add medicinal activated carbon of 0.05 times the weight of the eluent t...

Embodiment 2

[0022] After crushing the Epimedium plant, pass it through a 40-mesh sieve, add purified water 7 times the total weight of the Epimedium plant, heat and reflux for extraction twice, each time for 2.5 hours, and filter to obtain filtrate A; Add 14 times the total weight of Epimedium plants in an ethanol solution with a content of 70%, heat and reflux for extraction twice, each extraction for 2 hours, and obtain filtrate B by filtration; mix filtrate A and filtrate B, perform suction filtration, concentrate and recover under reduced pressure Ethanol to obtain the primary extract; the resulting primary extract is extracted with purified water, adjusted to PH2.2, left to stand, and suction filtered to obtain the icariin extract; the macroporous adsorption resin is used to treat the icariin extract obtained above For elution, the macroporous adsorption resin is eluted with 70% ethanol solution, 60% ethanol solution and 50% ethanol solution successively, and the eluate is collected a...

Embodiment 3

[0024] After crushing the Epimedium plant, pass it through a 40-mesh sieve, add purified water 8 times the total weight of the Epimedium plant, heat and reflux for extraction twice, each time for 3 hours, and filter to obtain filtrate A; Add 15 times the total weight of Epimedium plants in an ethanol solution with a content of 75%, heat and reflux for extraction twice, extract for 2 hours each time, and obtain filtrate B by filtration; mix filtrate A and filtrate B, perform suction filtration, concentrate and recover under reduced pressure Ethanol to obtain the primary extract; the obtained primary extract is extracted with purified water, adjusted to PH2.5, left to stand, and suction filtered to obtain the icariin extract; the obtained icariin extract is centrifuged with a centrifuge at 15000 Rotate / min and carry out centrifugation to obtain supernatant icariin extract; adopt macroporous adsorption resin to elute the above-mentioned icariin extract, and use concentration to be...

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Abstract

The invention discloses a method for simultaneously extracting icariin and baohuoside I and II from herba epimedii. The method comprises the following steps of: crushing herba epimedii plant and then passing the crushed herba epimedii plant through a screen mesh, adding alcohol liquor, heating for reflux extraction, and filtering to obtain filtrate B, concentrating under reduced pressure to recycle alcohol to obtain initial extract; extracting the initial extract by pure water, regulating PH, stewing and carrying out suction filtration to obtain icariin extract; carrying out elution on the icariin extract by adopting macroporous adsorption resin, carrying out elution by using alcohol and collecting eluant; adding medical active carbon into the eluant, heating and refluxing, cooling, and filtering to obtain filtrate C; separating, purifying and crystallizing the filtrate C by simulated moving bed chromatography to obtain herba epimedii crystal, and collecting filtrate D; drying the herba epimedii crystal to obtain a pure icariin finished product; adding tween into the filtrate D, carrying out enzymolysis by glucosaccharase or cellulose, and separating and purifying the enzymatic hydrolysate by a chromatographic column mixed and filled by the macroporous resin to obtain products baohuoside I and II.

Description

technical field [0001] The invention relates to a method for simultaneously extracting icariin and baohuoside I and II from epimedium. Background technique [0002] Dried stems and leaves of Epimedium brevicornum Maxim., Epimedium sagittatum Maxim., Epimedium pubescens Maxim., or Epimedium koreanum Nakai of Epimedium genus Berberidaceae . Epimedium mainly contains flavonoids, lignans, alkaloids, volatile oils, etc., wherein flavonoids mainly include icariin, icarisoside, etc.; Icariin, epimedoside (epimedoside) A, B, C, D, E, etc.; Glycosides, icariin-3-O-α-rhamnoside, quercetin, etc. At present, the main research content is the process of separating and purifying icariin. Icariin is mainly used to improve the function of cardiovascular and cerebrovascular systems, enhance the body's immunity, promote DNA synthesis, prevent and treat sexual dysfunction, etc. Icariin is also It has anti-aging and anti-tumor effects. [0003] Epimedium has male hormone-like effects, anti-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 刘海峰姚晨
Owner 成都合盛生物技术有限公司
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