Peony polypeptide as well as preparation method and application thereof

A peony polypeptide and peony seed technology, applied in the application of peptides and peptides, and the preparation of peony polypeptides, can solve problems such as hindering industrial applications, achieve good nutritional properties, promote fat metabolism, and enhance human immunity.

Active Publication Date: 2014-07-23
李杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Peony polypeptide refers to the oligopeptide mixture obtained by separating and refining the protein in the peony seed cake through indirect microbial fermentation or direct action of protease, but there is no mature process for its preparation at present, which hinders Therefore, in order to make full use of resources, adopt appropriate production technology to utilize these plant proteins, convert them into peptides, separate and purify them, and make peptide health products, which is of great significance to improving the added value and market competitiveness of the peony industry. important meaning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1: Grinding and pulverizing the peony cake with a pulverizer to 80 mesh, and the above peony cake is the cake after peony seeds are pressed to prepare peony seed oil.

[0027] Step 2 Degreasing Put the pulverized cake into a Soxhlet extractor for degreasing for 6 hours, then take it out and air-dry to obtain defatted peony cake powder for later use.

[0028] Step 3 Pulping Take 10g of defatted peony cake powder, add 90ml of 95°C hot water, stir evenly to fully dissolve the peony cake powder in the hot water.

[0029] Step 4 Sterilization and cooling Use a pressure steam sterilizer to sterilize at high temperature and high pressure for 15 minutes, and use it as a fermentation medium.

[0030] The Bacillus subtilis seed liquid of step 5 inoculation fermentation inoculates 10ml (contains Bacillus subtilis 3.4 * 10 in every mL seed liquid 7 cfu), pour into the fermentation medium, adjust the pH to 7.2, mix evenly with a glass rod, seal the bottle mouth with a fermenta...

Embodiment 2

[0038] Step 1: Grinding and pulverizing the peony cake with a pulverizer to 80 mesh, and the above peony cake is the cake after peony seeds are pressed to prepare peony seed oil.

[0039] Step 2 Degreasing Put the pulverized cake into a Soxhlet extractor for degreasing for 6 hours, then take it out and air-dry to obtain defatted peony cake powder for later use.

[0040] Step 3 Pulping Take 10g of defatted peony cake powder, add 90ml of 95°C hot water, stir evenly to fully dissolve the peony cake powder in the hot water.

[0041] Step 4 Sterilization and cooling Use a pressure steam sterilizer to sterilize at high temperature and high pressure for 20 minutes, and use it as a fermentation medium.

[0042] The mucormycetes seed solution of step 5 inoculation fermentation inoculates 10ml (contains mucormycetes 4 * 10 in every mL seed solution 7 cfu), pour into the fermentation medium, adjust the pH to 9, mix evenly with a glass rod, seal the bottle mouth with a fermentation seali...

Embodiment 3

[0050] Step 1 Grinding and pulverizing the peony cake with a pulverizer to 100 mesh, the above peony cake is the cake after peony seeds are pressed to prepare peony seed oil.

[0051] Step 2 Degreasing Put the pulverized cake into a Soxhlet extractor for degreasing for 6 hours, then take it out and air-dry to obtain defatted peony cake powder for later use.

[0052] Step 3 Pulping Take 10g of defatted peony cake powder, add 90ml of 95°C hot water, stir evenly to fully dissolve the peony cake powder in the hot water.

[0053] Step 4 Sterilization and cooling Use a pressure steam sterilizer to sterilize at high temperature and high pressure for 20 minutes, and use it as a fermentation medium.

[0054] Step 5 inoculation fermentation presses the Aspergillus oryzae seed liquid of inoculation 10ml (contains Aspergillus oryzae 4 * 10 in every mL seed liquid) 7cfu), pour into the fermentation medium, adjust the pH to 6.8, mix evenly with a glass rod, seal the bottle mouth with a fer...

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PUM

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Abstract

The invention relates to a method for preparing peony polypeptide from cake meal which is obtained through preparing peony seed oil by squeezing peony seeds, the polypeptide prepared by the method and application of the polypeptide. During the preparation of the polypeptide, various microbes are added to ferment or various proteases are added to be subjected to enzymolysis, so as to obtain the peony polypeptide product. The product can be in the form of powder, granules, tablets, capsules, oral liquid and the like. The peony polypeptide has the efficacy advantages of lowering cholesterol, lowering blood pressure, promoting fat metabolism, resisting fatigue, enhancing human immunity, regulating human physiological functions and the like and has broad development and application prospect in the fields of food industry, health care, medicine and the like.

Description

technical field [0001] The invention belongs to the technical field of peony seed cake deep processing, and in particular relates to a preparation method of peony polypeptide, the polypeptide prepared by the method and the application of the polypeptide. Background technique [0002] Peony seed oil was approved as a new resource food by the Ministry of Health in 2011, and then peony seed oil entered a large-scale production stage. After peony seed oil is prepared by pressing after peeling, a large amount of peony seed cakes will be produced, accounting for about one-third of the total amount of peony seeds. These seed cakes contain 5-15% fat, 30-40% protein, It also contains active ingredients such as paeoniflorin, benzoic acid, and polysaccharides. Therefore, making full use of the active and nutritional ingredients in these residues is of great significance for the comprehensive utilization of peony seeds and the improvement of the added value of the peony industry. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/415C07K1/34A23L1/305A23L2/38A23C11/10
Inventor 李杰胡良富
Owner 李杰
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