Transmissive Quantified Phase and Fluorescence Combined Imaging Microscopy

A microscope and transmission technology, applied in the field of optics, can solve problems such as inconvenient research work, inability to fuse fluorescence images, and difficulty in obtaining precise positioning of target molecules

Inactive Publication Date: 2016-06-01
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Problems solved by technology

[0005] However, in the process of realizing the present invention, the applicant found that the current microscope is difficult to obtain the precise positioning of the target molecule in the cell, that is, it is impossible to fuse the fluorescence image and the cell structure image to realize the imaging of cell structure and function, thus causing research work. the inconvenience

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  • Transmissive Quantified Phase and Fluorescence Combined Imaging Microscopy
  • Transmissive Quantified Phase and Fluorescence Combined Imaging Microscopy
  • Transmissive Quantified Phase and Fluorescence Combined Imaging Microscopy

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Embodiment Construction

[0036] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0037] It should be noted that, in the drawings or descriptions of the specification, similar or identical parts all use the same figure numbers. Implementations not shown or described in the accompanying drawings are forms known to those of ordinary skill in the art. Additionally, while illustrations of parameters including particular values ​​may be provided herein, it should be understood that the parameters need not be exactly equal to the corresponding values, but rather may approximate the corresponding values ​​within acceptable error margins or design constraints. In addition, the directional terms mentioned in the following embodiments, such as "upper", "lower", "front", "rear", "left", "right", etc., are only re...

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Abstract

The invention provides a transmission quantization phase and fluorescence combined imaging microscopy. The microscope comprises a light source system 100, a first beam splitting assembly 200, a reference light path 300, an object light path 400, a fifth light splitting prism 500, an interference imaging system 600 and a fluorescence imaging system 700. According to the invention, a fluorescence imaging technology and a quantization phase imaging technology are combined, thus simultaneous imaging of a cell structure and a fluorescence molecule is realized.

Description

technical field [0001] The present invention relates to the field of optics, in particular to a transmission quantitative phase and fluorescence combined imaging microscope that can simultaneously realize cell morphology and structure imaging and fluorescence function imaging, and has good application prospects in the observation of cells and crystal growth. . Background technique [0002] Life science is a discipline that mainly focuses on experimental observation. Experimental methods and tools determine the true degree and resolution of observation: the appearance of optical microscopes promotes the birth of general cell biology, and the appearance of electron microscopes leads to the birth of cell ultrastructure. academic research. [0003] In the past two years, in terms of optical microscopy, photo-activated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), and stimulated emission depletion microscopy ( Stimulated emission depletio...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/06G02B21/36G01N21/64G01N21/45
Inventor 罗志勇付彦辉张翔纪伟贾策仓怀兴徐涛
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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