Semi-contact under-oil continuous droplet sample applying and liquid adding method

Abstract

The invention provides a semi-contact under-oil continuous droplet sample applying and liquid adding method, which is suitable for sequentially operating a droplet array system. According to the method, the distance between the pointed end of a capillary tube sample applying needle and the lower surface of a micro-hole (or a generated droplet) is controlled accurately, and the affinity or interface tension interaction between the droplet and the surface (or the generated droplet) is utilized, so that rapid and reliable continuous droplet sample applying or continuous liquid adding is realized, and the problem of cross infection during sample applying is solved effectively. The method is suitable for biochemical analysis screening researches such as high-flux medicament screening, protein crystallization condition screening, enzyme kinetics research and drug toxicity determination.

Description

technical field

[0001] The field of the invention relates to the field of liquid droplet analysis, in particular to a semi-contact method for continuously spotting and adding liquid to liquid droplets under oil. Background technique

[0002] Droplet-based microfluidics has achieved rapid development and wide application. The main advantages of droplet microfluidics include: 1) The volume of the droplet reactor can be flexibly adjusted from femtoliter to nanoliter, so the consumption of samples and reagents can be reduced to an extremely low level; 2) It can be generated in a short time A large number of droplet reactors, thereby significantly improving the throughput of analysis and screening; 3) The rapid mass transfer, heat transfer and mixing of components can be realized in the droplets, which effectively improves the efficiency of biochemical reactions; 4) The encapsulation of the oil phase, Effectively reduce the evaporation, diffusion and cross-contamination of trace...

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