A new atmospheric and room temperature plasma mutagenesis breeding device

Abstract

The invention relates to a novel normal-pressure room-temperature plasma induced mutation breeding device. The novel normal-pressure room-temperature plasma induced mutation breeding device comprises a sample processing system, a cooling system, a control system and a detection system; the sample processing system comprises a clean working chamber without bioactive contaminants; a step motor and an object stage are arranged in the clean working chamber; the object stage is arranged on the step motor; at least one sterilizing device mounting position is reserved in the inner cavity or on the wall of the clean working chamber; the detection system comprises a gas flow controller, a temperature sensor and a position sensor; and the control system comprises an operating panel and a controller, and the controller is used for controlling the step motor to realize rising and falling or horizontal rotation of the object stage so as to automatically process a plurality of samples. The novel normal-pressure room-temperature plasma induced mutation breeding device is capable of realizing the functions of continuous automatic processing, automatic sterilization, automatic monitoring and control on the samples, and also capable of completing the plasma induced mutation breeding in the normal pressure and room temperature environment at higher efficiency.

Description

technical field [0001] The invention belongs to the field of plasma technology processing equipment, in particular to a novel atmospheric pressure room temperature plasma mutation breeding device for biological breeding. Background technique [0002] High-quality microbial strains are always the core of the biological industry. How to use efficient biological mutagenesis technology to achieve rapid optimization of strains is an important task for the biological industry. Conventional biological mutagenesis methods generally have problems such as low work efficiency, heavy workload, and large blindness. Chemical mutagenesis is also likely to cause bodily harm to operators and pollute the environment. Physical mutagenesis has poor mutagenic performance and microorganisms After repeated use, a certain tolerance is produced. Other methods require professional and complex equipment, and some methods are very expensive to operate. Therefore, conventional biological mutagenesis met...

AI Technical Summary

Problems solved by technology

The application of this technology in the field of biological breeding is limited due to the complex physical process of plasma, nonlinearity, strong coupling, variable load, etc., and the difficulty of forming plasma under normal pressure and room temperature.

Moreover, the penetration ability of the plasma itself is limited and the action distance is short. Therefore, in the actual application process, it is necessary to cooperate with other chemical substances (such as hydrogen peroxide, ozone, etc.) to achieve the disinfection effect.

At present, experimental devices either process biological samples in an open space, or although they are relatively closed spaces, the closed space cannot be sterilized or effectively isolated from the external environment during operation, which may easily lead to contamination of biological samples, serious Affects the normal use of plasma devices in research and production

The surface of the plasma device is disinfected by manual wiping, but the cleanliness of the entire discharge space and process cannot be guaranteed

Moreover, the amount of sample processing is small, which is not conducive to large-scale operations. After processing a single sample, it is necessary to open the operation room to take it out manually, and then replace the next sample to be processed. The steps are cumbersome and the risk of bacterial contamination is high, and the labor cost is increased. , reducing the efficiency of mutagenesis breeding

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