A preparation method of aptamer-induced ag2s quantum dots
An aptamer and quantum dot technology, applied in the field of material chemistry, can solve the problems of reduced product quantum yield, poor product biocompatibility, harsh preparation conditions, etc., and achieves the effects of environmental friendliness, simple operation and mild reaction conditions.
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Embodiment 1
[0023] The aptamer S2.2 whose end was modified with 6 cytosine Cs was dispersed in deionized water to obtain an aqueous aptamer solution with a concentration of 5 μmol / L. Add 1 mol / L AgNO to the solution 3 10 μL aqueous solution, stirred at room temperature for 1-5 min, then added 1 mol / L Na 2 S aqueous solution 2 μL, stirred at room temperature for 1-10 min, and the product was dried to obtain Ag 2 S quantum dots, the average particle size is about 2 nm.
Embodiment 2
[0025] The aptamer S2.2, whose end was modified with 12 cytosine Cs, was dispersed in deionized water to obtain an aqueous aptamer solution with a concentration of 10 μmol / L. Add 1 mol / L AgNO to the solution 3 20 μL aqueous solution, stirred at room temperature for 1-5 min, then added 1 mol / L Na 2 S aqueous solution 5 μL, stirred at room temperature for 1-10 min, and the product was dried to obtain Ag 2 S quantum dots, the average particle size is about 2 nm.
Embodiment 3
[0027] The aptamer S2.2, whose end was modified with 18 cytosine Cs, was dispersed in deionized water to obtain an aqueous aptamer solution with a concentration of 20 μmol / L. Add 1 mol / L AgNO to the solution 3 40 μL aqueous solution, stirred at room temperature for 1-5 min, then added 1 mol / L Na 2 S aqueous solution 5 μL, stirred at room temperature for 1-10 min, and the product was dried to obtain Ag 2 S quantum dots, the average particle size is about 2 nm.
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