A coronoid fermented rice milk health drink and preparation method thereof
A technology of R. coronoides and health-care beverage, which is applied in the field of rice milk health-care beverage and its preparation
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Embodiment 1
[0024] This embodiment includes the following steps:
[0025] (1) Preparation of dark tea extract: Weigh 250g of black tea, add 4L of boiling water, extract at 90°C for 30 minutes, filter, wash the filter residue with boiling water for 3 times, filter, combine the filtrates, and dilute to 5L to prepare Obtain the black tea extract of 5g black tea / 100mL;
[0026] (2) Preparation of S. coronoidis seed liquid: Dilute 5g black tea / 100mL black tea extract obtained in step (1) to 0.8g black tea / 100mL black tea extract, and then add 0.8g black tea Add 3% sucrose equivalent to its weight to the tea / 100mL dark tea extract, adjust the pH to 5.0, sterilize at 115°C for 15 minutes, and cool to obtain a liquid medium for seed liquid preparation. Under sterile conditions, use an inoculation loop to pick up the spores produced by Trichomonas coronis that have been cultured on the PDA slope for 4 days, and insert them into the liquid medium (the inoculation amount is 3 rings of spores / 100mL ...
Embodiment 2
[0036] This embodiment includes the following steps:
[0037] (1) Preparation of dark tea extract: Weigh 250g of black tea, add 4.5L of boiling water, extract at 92°C for 20 minutes, filter under reduced pressure, rinse the residue twice with boiling water, filter, combine the above filtrates, and dilute to 5L, that is, to prepare 5g black tea / 100mL black tea extract;
[0038] (2) Preparation of S. coronoidis seed liquid: Dilute 5g black tea / 100mL black tea extract obtained in step (1) to 0.8g black tea / 100mL black tea extract, add 0.8g black tea Add sucrose equivalent to 4% of its weight in 100mL black tea extract, adjust the pH to 5.5, sterilize at 115°C for 15 minutes, and cool to obtain a liquid medium; The spores produced by P. coronis that were cultured on the PDA slant for 5 days (the inoculation amount is 2 ring spores / 100ml liquid medium), were inserted into the liquid medium, and cultured at 27°C and 150r / min for 50h to obtain the seeds liquid;
[0039](3) Soaking...
Embodiment 3
[0047] This embodiment includes the following steps:
[0048] (1) Preparation of black tea extract: Weigh 250g of black tea, add 4.2L of boiling water, extract at 95°C for 25 minutes, filter under reduced pressure immediately, rinse the residue with boiling water for 3 times, filter, combine the above filtrates, and constant volume to 5L, that is, 5g black tea / 100mL black tea extract is prepared; the extract is diluted with water to the required concentration before use;
[0049] (2) Preparation of S. coronoidis seed liquid: Dilute 5g black tea / 100mL black tea extract obtained in step (1) to 0.8g black tea / 100mL black tea extract, add 0.8g black tea Add 5% sucrose equivalent to its weight in 100mL black tea extract, adjust the pH to 6, sterilize at 115°C for 15 minutes, and cool to obtain a liquid medium for seed liquid preparation; Dip the spores produced by the PDA slant cultured for 7 days (the inoculation amount is 2 ring spores / 100ml liquid medium), insert them into the ...
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