Methods of Promoting Cattle Oestrus and Improving Conception Rate of Cattle Breeding
A technology of conception rate and inhibin, which is applied in the field of promoting estrus and ovulation to increase the conception rate of breeding, can solve the problems of uncontrollable egg discharge and embryo quality and the function of corpus luteum, so as to improve the conception rate of estrus and breeding, and promote conception efficiency and reduce production costs
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Embodiment 1
[0020] The preparation of inhibin recombinant fusion protein oil emulsion vaccine was prepared according to the method disclosed in Example 1 of patent CN201010618516.4, and the concentration of porcine inhibin recombinant fusion protein in the vaccine was 1-2 mg / mL.
Embodiment 2
[0022] For the immunization and TAI treatment of cows with normal production performance, 89 cows in good body condition were selected and divided into two groups, including 61 cows in the experimental group and 28 cows in the control group.
[0023] Experimental method: On the first day, intramuscular injection of inhibin recombinant fusion protein oil emulsion vaccine to the experimental group, 1ml / head, injection of normal saline, 1ml / head to the control group, and 100μg GnRH injection to all cows at the same time; Cows were injected with 0.3 mg cloprostenol; on the ninth day, all cows were injected with 100 μg GnRH; on the 10th to 11th day, all cows were identified for estrus and artificially inseminated.
[0024] Experimental results: On the 42nd day, the pregnant cows of the experimental group and the control group were diagnosed, and the diagnostic results are shown in Table 1:
[0025] Table 1 Statistical conception rate of normal cows in different treatment groups
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Embodiment 3
[0032] The immunization and TAI of female buffaloes were divided into two groups: in summer and autumn, respectively.
[0033] Preparation of female buffaloes: In summer, select 24 Mora-Swamp hybrid female buffaloes aged 5-7 years old and in good condition. Divide the female buffaloes into two groups, 11 in the experimental group and 13 in the control group. head.
[0034] Experimental method: On the first day, the experimental group was injected intramuscularly with statin recombinant fusion protein oil emulsion vaccine 2ml / head, and the control group was injected with normal saline, 2ml / head, and at the same time, all buffaloes were injected with 100μg GnRH; on the seventh day, all buffaloes were injected with 0.3 mg cloprostenol; on the ninth day, all buffaloes were injected with 100 μg GnRH; on the 10th to 11th day, all buffaloes were identified for estrus and artificially inseminated, and on the 42nd day, pregnancy diagnosis was performed;
[0035] In autumn, 12 same fem...
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