Fermentation process of ocean penicillium and secondary metabolite Flufuran thereof

Abstract

The invention discloses a fermentation process of ocean penicillium and secondary metabolite Flufuran thereof. The ocean penicillium is characterized in that the preservation number of penicillium is CCTCCM2014087, and the fermentation process of the penicillium comprises the following steps: inoculating PDA (Potato Dextrose Agar) with the ocean penicillium, culturing for 2 days in an incubator with the temperature of 25 DEG C, picking a single bacterial flora and then inoculating on a PDA culture medium, and culturing for 2 days at the temperature of 25 DEG C for later use; (2) inoculating PDB with the activated strain, culturing for 1 day at the temperature of 25 DEG C and at a rotating speed of 150rpm to obtain a seed solution; (3) inoculating the seed solution in a fermentation culture medium and culturing for 12 days at the temperature of 25 DEG C and at the rotating speed of 150rpm, wherein the inoculated amount of the seed solution is 5vol%,; (4) filtering a fermentation solution to obtain a supernatant liquor, extracting for three times by use of ethyl acetate, removing an organic phase, and dissolving again by use of methanol to obtain a sample introduction solution; (5) separating and purifying the sample introduction solution by adopting a high-speed counter-current chromatographic method to obtain a product, wherein the fermentation process has the advantages of high preparation speed and high product purity.

Description

technical field

[0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation process of marine Penicillium and its secondary metabolite Flufuran. Background technique

[0002] Flufuran was first obtained from Gabriela M. Cabrera et al. in 2002 Polyporus ciliatus isolated from (Gabriela M.; Robertia M.; Jorge E.; Alicia M. Phytochem. 2002, 61, 189-193.). Antonio Evidente et al. 2009 from Aspergillus flavus and found that the compound had significant activity against Phytophthora (Antonio E.; Gennaro C.; Biancavaleria P.; Anna A.; Antonino T.; Dominique M. Chem. Biodivers. 2009, 6, 328-334.). In addition, analogues of this compound, such as 2-tetrahydrofurancarboxylic acid, are widely used as pharmaceutical intermediates.

[0003] In the literature mentioned above, the fermentation yields of Flufuran are 0.625 mg / L and 43.7 mg / L, and they need to be cultured for 15 days and 14 days, respectively. Therefore, low yield i...

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