Function and application of Carabin for treating cardiac hypertrophy

A carabin, 1. carabin technology, applied in the field of gene function and application, can solve the problem of incomplete interpretation of the mechanism of cardiac hypertrophy, achieve the effect of inhibiting cardiac hypertrophy and protecting heart function

Inactive Publication Date: 2014-10-22
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing studies cannot fully explain the mechanism of cardiac hypertrophy

Method used

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  • Function and application of Carabin for treating cardiac hypertrophy
  • Function and application of Carabin for treating cardiac hypertrophy
  • Function and application of Carabin for treating cardiac hypertrophy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Acquisition of Cardiac Hypertrophy (AB) Model

[0035] 1. Grouping of experimental animals: heart-specific Carabin transgenic mice (TG) and non-transgenic mice (NTG), and aortic constriction to establish a model of myocardial hypertrophy. Randomly divided into 4 groups, grouped as follows: non-transgenic mouse sham operation group (NTG Sham) and AB operation group (NTG AB), heart-specific Carabin transgenic mouse sham operation group (TG Sham) and AB operation group (TG AB ).

[0036] 2. The myocardial hypertrophy model adopts aortic arch coarctation surgery, and the operation process of the model is as follows:

[0037] 2.1 Preoperative preparation

[0038] (1) Anesthesia: First weigh the mice, calculate the required amount of anesthetic (3% pentobarbital sodium) according to 90 mg / kg body weight, inject intraperitoneally, and record the injection time point. There is no obvious reaction between tail and toe pinching and the mouse is in good condition. Thi...

Embodiment 2

[0045] Example 2 Detection of cardiac hypertrophy and fibrosis in mouse cardiac hypertrophy (AB) model

[0046] 1. Collect materials

[0047] (1) Preliminary work: prepare in advance a urine cup filled with 20mL of 10% formaldehyde by volume, and label it (mouse number, group, type of operation and date of collection). A petri dish filled with 10% KCl solution was placed at the sampling site. Turn on the analytical balance and set it to zero for later use. Mice were then weighed and sacrificed.

[0048] (2) Material collection: Ophthalmic curved forceps clamped the vascular pedicle below the atrial appendage, cut off the heart, and quickly placed it in 10% KCl solution by mass fraction. After the heart stops beating in the diastolic phase, place it on sterilized gauze, gently squeeze the fluid in the heart cavity, dip the surface fluid dry, weigh and record, put the heart into the corresponding urine cup, fix it for 48 hours and use it for pathological testing.

[0049] (...

Embodiment 3

[0060] Example 3 Cardiac function detected by ultrasound in model mice with myocardial hypertrophy (AB)

[0061] 1 Preliminary preparation

[0062] (1) Anesthesia machine preparation: first connect the oxygen cylinder and the air inlet port on the anesthesia machine, then unscrew the sealing cap of the dosing port on the anesthesia machine, quickly add isoflurane to the safety scale, and then tighten the sealing cap. Unscrew the main valve on the oxygen cylinder, adjust the knob of the flow control valve, and maintain the outlet pressure at 0.2-0.3mPa.

[0063] (2) Preparation of the mice to be tested: After the mice to be tested were quickly anesthetized with isoflurane, the hair on the left chest area was shaved, and the head of the treated mice was inserted into the anesthetic catheter sleeve, and treated with 1.5-2.0% isoflurane Alkane maintains a stable anesthesia in mice.

[0064] 2 Cardiac function test

[0065] The mice were placed in the left decubitus or supine posi...

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Abstract

The invention discloses a function and application of Carabin for treating cardiac hypertrophy, belonging to the field of functions and applications of genes. By virtue of the function and application of Carabin for treating cardiac hypertrophy, cardiac specific Carabin transgenic mice and non-genetically modified organisms are used as experiment targets; a relation between the expression of Carabin gene and the cardiac hypertrophy is researched by simulating a cardiac hypertrophy disease model by virtue of an aortic arch narrow operation; results show that the cardiac hypertrophy and fibrosis can be remarkably suppressed by over expression of the Carabin gene, and the heart function can be improved, namely that the Carabin gene has the functions of protecting the heart function and suppressing the cardiac hypertrophy. By virtue of the function of the Carabin gene, Carabin can be used for preparing medicines for protecting the heart function and / or preventing, relieving and / or treating the cardiac hypertrophy, so that an effective novel way is provided for the treatment of the cardiac hypertrophy.

Description

technical field [0001] The invention belongs to the field of gene function and application, and in particular relates to the function and application of Carabin (TBC1D10C) in the treatment of cardiac hypertrophy, specifically the application in the preparation of drugs for preventing, alleviating and / or treating cardiac hypertrophy. Background technique [0002] According to the report of the World Health Organization, cardiovascular disease has become one of the global chronic non-communicable diseases that threaten human life and health. At present, the mortality rate of chronic heart failure is increasing rapidly, and it has undoubtedly become one of the important diseases that threaten human life. Basic and clinical studies have confirmed that heart failure is often evolved from Cardiac Hypertrophy. Studies have shown that myocardial hypertrophy is a common symptom of many cardiovascular diseases such as coronary heart disease, hypertension, arrhythmia, valvular heart di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P9/00
Inventor 李红良蒋丁胜邓克穷张晓东
Owner WUHAN UNIV
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