Application of reagents for detecting ccl15 chemokines in preparation of reagents for screening thyroid follicular carcinoma

Abstract

The invention belongs to the field of biological medicine, and particularly relates to the application of CCL15 chemotactic factor in the preparation of follicular thyroid carcinoma (FTC) screening reagent. According to the application, a fresh thyroid follicular tumor tissue sample which is confirmed to be FTC is taken as a research object, and protein extraction, cell factor antibody chip detection, cell culture, cell chemotaxis experiment, antibody neutralization test and the like are carried out on the research object, so that the high expression of CCL15 in the FTC is verified, the infiltrating density of macrophage in the CCL15 high-expression group is remarkably larger than that of the macrophage in the CCL15 low-expression group, and the FTC cultural supernatant is capable of promoting the migration of Thp-1 mononuclear cell strain. Therefore, CCL15 can be taken as a potential biomarker and can be applied to the FTC screening reagent; furthermore, after the CCL15 is applied to the FTC screening reagent, experimental data and theoretical basis can be provided.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the application of CCL15 chemokine in the preparation of reagents for screening thyroid follicular carcinoma. Background technique [0002] As one of the types of differentiated thyroid cancer, follicular thyroid carcinoma (FTC) is second only to papillary thyroid carcinoma in incidence. Most well-differentiated FTCs are almost indistinguishable from follicular adenoma (FA) in terms of clinical manifestations and imaging features; fine-needle aspiration (FNA) cannot reliably identify follicles in terms of cytology Whether the tumor infiltrates the capsule or invades capsular vessels cannot be used to distinguish follicular carcinoma from adenoma. [0003] Similarly, it is impossible to thoroughly examine the capsule when performing frozen section diagnosis during the operation, so a definite diagnosis can only be made after taking more samples. Histopathologically, the so...

AI Technical Summary

Problems solved by technology

The widely infiltrating follicular carcinoma of the thyroid is usually easy to confirm the invasive growth under the macroscopic and light microscope. The tumor can invade outside the thyroid gland, and it is prone to recurrence and hematologic metastasis after surgical resection. The prognosis is poor, and the 10-year survival rate is only 25% to 45%.

[0004] However, for patients diagnosed as "follicular thyroid tumor in the frozen section pathology report, it is necessary to wait for paraffin section to determine whether the tumor has capsule and / or vascular invasion", they are faced with the possibility of a second operation

It is precisely because of the difficulty in distinguishing between thyroid follicular carcinoma and thyroid adenoma that it is difficult for surgeons to formulate an appropriate surgical plan

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