41 results about "Cell chemotaxis" patented technology

The invention discloses a cells invasion and chemotaxis model as well as a preparation method thereof, which comprises the following steps: 2)culturing cells; 2)configuring a lower layer matrix glue; 3) configuring maker 1; 4)configuring an intermediate cell layer; 5) configuring maker 2; and configuring an upper layer matrix glue. The cells invasion and chemotaxis model can visually obtain the powerful evidence for cells chemotaxis and migration based on overcoming the gravity, and can perform HE coloration, imunohistochemistry and immunofluorescence, so that cell associated protein expression condition can be observed.

The invention relates to the technical field of biology, and in particular relates to a cell chemotaxis analysis chip. The cell chemotaxis analysis chip comprises a solution inlet chamber, a chemotaxis channel, a barrier channel and a solution absorption chamber, wherein the solution inlet chamber is provided with a solution inlet for feeding a cell solution and is used for accommodating the cell solution, one end of the chemotaxis channel is communicated with the liquid inlet chamber, the other end of the chemotaxis channel is communicated with one end of the barrier channel, the inner diameter of the chemotaxis channel is greater than the diameter of each cell contained in the cell solution, the inner diameter of the barrier channel is less than the diameter of each cell contained in the cell solution, the other end of the barrier channel is communicated with the solution absorption chamber, and the solution absorption chamber is used for absorbing the solution in the cell solution. The invention also relates to a use method and a preparation method of the cell chemotaxis analysis chip. According to the cell chemotaxis analysis chip provided by the invention, a cell chemotaxis process at a specific concentration gradient can be quantitatively observed very conveniently, and the influence on the chemotaxis process by flow is eliminated to facilitate the research on the cell chemotaxis behavior.

The invention belongs to the field of biological medicine, and particularly relates to the application of CCL15 chemotactic factor in the preparation of follicular thyroid carcinoma (FTC) screening reagent. According to the application, a fresh thyroid follicular tumor tissue sample which is confirmed to be FTC is taken as a research object, and protein extraction, cell factor antibody chip detection, cell culture, cell chemotaxis experiment, antibody neutralization test and the like are carried out on the research object, so that the high expression of CCL15 in the FTC is verified, the infiltrating density of macrophage in the CCL15 high-expression group is remarkably larger than that of the macrophage in the CCL15 low-expression group, and the FTC cultural supernatant is capable of promoting the migration of Thp-1 mononuclear cell strain. Therefore, CCL15 can be taken as a potential biomarker and can be applied to the FTC screening reagent; furthermore, after the CCL15 is applied to the FTC screening reagent, experimental data and theoretical basis can be provided.