5 results about "Cell chemotaxis" patented technology

The present application relates to a method for constructing a human acute liver injury and chemotaxis model based on a normal liver organoid and application thereof. Specifically, the present application provides a method for constructing a human liver organoid; further, contacting the liver organoid with a hepatotoxic drug can construct a human acute liver injury model; co-culturing the organoid or the injury model with immune cells can construct an in vitro immune cell chemotaxis model. The present application also provides reversible immunoaffinity magnetic beads for isolating VSIG4-positive macrophages and a method for isolating the same. The human liver organoid, acute liver injury model and chemotaxis model of the present application can be used for screening drugs for treating acute liver injury, evaluating the hepatotoxicity of compounds, researching the pathogenesis of acute liver injury and evaluating the effect of immunomodulatory drugs.

This invention relates to the field of medical image processing technology, specifically to a method and system for processing immune scoring data based on deep learning. The method includes: acquiring an immunohistochemically stained whole-section image to be scored; dividing the whole-section image into non-overlapping image blocks; performing multi-level spectral separation and decomposition on each image block to construct a spatial density distribution map of immune cells in the whole-section; segmenting the tumor central region and invasion edge region on the spatial density distribution map according to tissue morphology gradients, and extracting the corresponding local density distribution. This invention, by inverting the effective chemotaxis coefficient and effective proliferation coefficient of the reaction-diffusion coupling system from the spatial density distribution map of immune cells, can directly quantify the degree of mechanistic deviation of immune cell chemotaxis and proliferation capacity in the tumor microenvironment, overcoming the limitations of traditional methods that rely solely on static cell density counting, and giving the scoring a clear biophysical meaning.

The invention relates to the technical field of in-vitro cell model construction, and particularly discloses an in-vitro airway allergen presentation model and a construction method and application thereof. A double-catheter structure is prepared by adopting a 3D printing method, the inner tube is inoculated with human pulmonary epithelial cells, the bottom end of the inner tube is closed to reconstruct an airway mucosa physical barrier in a bionic manner, and the outer tube loads, induces and activates an innate immune cell subset to simulate an airway mucosa immune surveillance microenvironment; the double catheters are placed in a culture medium system containing peripheral blood mononuclear cells or / and CD4 + T cells, a culture medium containing to-be-detected allergens is added into the inner tube for multi-cell co-culture, and a highly-bionic in-vitro airway allergen presentation model is constructed. Multi-index detection of immune cell chemotaxis, T cell infiltration, cytokine secretion and T cell differentiation is achieved through immunofluorescence, ELISA and flow cytometry at the same time, diversified requirements of mechanism research and drug screening are met, and detection dimensions are comprehensive.

Disclosed is a method for modifying a fibroblast. The method comprises the following steps: collecting peripheral blood from a healthy person, extracting peripheral blood mononuclear cells from the peripheral blood in vitro by using a lymphocyte separation medium, and sorting and purifying B cells by using immunomagnetic beads; and constructing human CXCL13, TNFSF13B and VCAM1 overexpression plasmids by using molecular cloning technology, and infecting MRC-5 with a lentivirus to obtain an MRC-5 fibroblast simultaneously overexpressing CXCL13, TNFSF13B and VCAM1. Further provided is a modified fibroblast obtained on the basis of the method. Further provided is the use of the modified fibroblast in terms of inducing B-cell chemotaxis, inducing B-cell adhesion and promoting antibody secretion in B cells. The modified fibroblast can be used in the chemotaxis and adhesion of B cells, promotes antibody secretion in B cells, and has good clinical application value.

The application relates to the fields of biomedical engineering and microfluidic technology, and particularly relates to an organ-on-chip model, which comprises a substrate, the substrate is provided with cell culture chambers and perfusion channels, the cell culture chambers and the perfusion channels are both provided with a plurality of, the perfusion channels are arranged between the cell culture chambers and are communicated with the cell culture chambers through permeable membranes, the perfusion channels comprise first perfusion channels and second perfusion channels, one end of the first perfusion channels is provided with a first fluid injection hole, the other end is provided with a first fluid collection hole, one end of the second perfusion channels is provided with a second fluid injection hole, the other end is provided with a second fluid collection hole. The organ-on-chip model can be flexibly assembled with different accessories, can meet the culture requirements of different cell sample types, can more truly simulate the influence of drugs or other substances on the physiological functions of organs, and can be used in cell chemotaxis experiments.