40 results about "Cell chemotaxis" patented technology

Low-cost and portable microfluidic systems are needed for cell migration research and Point of Care (POC) testing. This study introduces a low-cost and portable USB Microscope Microfluidic Chemotaxis Analysis System (UMCAS) for rapid analysis of cell chemotaxis studies. A standalone microfluidic gradient generator is also developed for rapid generation of chemical gradient in microfluidic device without need of any peripheral perfusion apparatus. A smart phone based application program was developed for the real-time remote monitoring of the migration data. This system is validated by observing the neutrophil migration in three different conditions: 1) medium control, 2) uniform IL-8 control, and 3) IL-8 gradient. The results show that neutrophils exhibit random migration in both medium and uniform IL-8 control experiments, while they show strong directional migration to the IL-8 gradient. These results successfully validated the developed UMCAS system.

Low-cost and portable microfluidic systems are needed for cell migration research and Point of Care (POC) testing. This study introduces a low-cost and portable USB Microscope Microfluidic Chemotaxis Analysis System (UMCAS) for rapid analysis of cell chemotaxis studies. A standalone microfluidic gradient generator is also developed for rapid generation of chemical gradient in microfluidic device without need of any peripheral perfusion apparatus. A smart phone based application program was developed for the real-time remote monitoring of the migration data. This system is validated by observing the neutrophil migration in three different conditions: 1) medium control, 2) uniform IL-8 control, and 3) IL-8 gradient. The results show that neutrophils exhibit random migration in both medium and uniform IL-8 control experiments, while they show strong directional migration to the IL-8 gradient. These results successfully validated the developed UMCAS system.

The invention discloses a cells invasion and chemotaxis model as well as a preparation method thereof, which comprises the following steps: 2)culturing cells; 2)configuring a lower layer matrix glue; 3) configuring maker 1; 4)configuring an intermediate cell layer; 5) configuring maker 2; and configuring an upper layer matrix glue. The cells invasion and chemotaxis model can visually obtain the powerful evidence for cells chemotaxis and migration based on overcoming the gravity, and can perform HE coloration, imunohistochemistry and immunofluorescence, so that cell associated protein expression condition can be observed.

The invention discloses a CMKLR1 antagonistic polypeptide and derivatives and application thereof, and particularly relates to analogs of the CMKLR1 antagonistic polypeptide shown in sequences SEQ IDNO.1-17 and derivatives thereof. The derivatives of the binding peptide are products obtained after conventional modification of amino terminals or carboxyl terminals on CMKLR1 binding peptide amino acid side chain groups and CMKLR1 antagonistic polypeptide fragments. The binding peptide and the derivatives thereof can be bonded with CMKLR1 in vitro, and by blocking the bond of chemerin and CMKLR1, cAMP concentration increase is promoted, calcium influx caused by chemerin is inhibited and cell chemotaxis caused by chemerin is inhibited. Besides, proliferation of ovarian cancer cells can be inhibited, the cell cycle is blocked, apoptosis of the ovarian cancer cells is promoted, and effective small-molecular treatment drugs are provided for female reproductive diseases such as ovarian cancer.

The present invention relates to an improvement in an apparatus for detecting chemotaxis of cells. It aims at providing a structure for detecting chemotaxis of cells at an elevated accuracy with the use of a microquantity of cells. That is to say, an object of the present invention is to provide an apparatus for detecting chemotaxis of cells by which cell injection and position control can be easily carried out while ensuring the prevention of unexpected migration of the cells definitely positioned in a well or the injected sample so that a stable concentration gradient due to the diffusion of the specimen can be maintained and which ensures further automated operation and controlling.Namely, an apparatus for detecting chemotaxis of cells with a structure wherein two wells are connected to each other via a channel having resistance to the passage of cells and each well has an opening for injecting cells or a specimen, characterized by having (1) a means of transporting a liquid and a means of stopping the transportation after the injection or the aspiration discharge of the liquid and (2) a means of sealing the opening(s) in one or both of the cell-injection side and the specimen-injection side.

The invention relates to the technical field of biology, and in particular relates to a cell chemotaxis analysis chip. The cell chemotaxis analysis chip comprises a solution inlet chamber, a chemotaxis channel, a barrier channel and a solution absorption chamber, wherein the solution inlet chamber is provided with a solution inlet for feeding a cell solution and is used for accommodating the cell solution, one end of the chemotaxis channel is communicated with the liquid inlet chamber, the other end of the chemotaxis channel is communicated with one end of the barrier channel, the inner diameter of the chemotaxis channel is greater than the diameter of each cell contained in the cell solution, the inner diameter of the barrier channel is less than the diameter of each cell contained in the cell solution, the other end of the barrier channel is communicated with the solution absorption chamber, and the solution absorption chamber is used for absorbing the solution in the cell solution. The invention also relates to a use method and a preparation method of the cell chemotaxis analysis chip. According to the cell chemotaxis analysis chip provided by the invention, a cell chemotaxis process at a specific concentration gradient can be quantitatively observed very conveniently, and the influence on the chemotaxis process by flow is eliminated to facilitate the research on the cell chemotaxis behavior.

The invention discloses a CMKLR1 antagonistic polypeptide and its derivatives and applications, specifically related to the sequence of SEQ ID No.1‑9 and its derivatives. The derivatives of the binding peptide are CMKLR1 binding peptide amino acid side chain group, CMKLR1 The product obtained by conventional modification of the amino-terminal or carboxyl-terminal of the antagonistic polypeptide fragment, or the product obtained by linking a tag for polypeptide or protein detection or purification on the CMKLR1 antagonistic polypeptide; the binding peptide and its derivatives can bind CMKLR1 in vitro , by blocking the combination of chemerin / RvE1 and CMKLR1 to change the concentration of cAMP, inhibit the calcium influx caused by chemerin, and inhibit the cell chemotaxis caused by chemerin, and provide effective therapeutic small molecule drugs for diseases with high expression of CMKLR1 receptors.

N-(2-aryl-propionyl)-amides of formula (I) are described. The process for their preparation and pharmaceutical preparations thereof are also described. The amides of the invention are useful in the prevention and treatment of tissue damage due to the exacerbate recruitment of polymorphonuclear neutrophils (leukocytes PMN) at the inflammatory sites. In particular, the invention relates to the R enantiomers of N-(2-aryl-propionyl)amides of formula (I) for use in the inhibition of the chemotaxis of neutrophils induced by IL-8. The compounds of the invention are used in the treatment of psoriasis, ulcerative cholitis, glomerular nephritis, acute respiratory insufficiency, idiopathic fibrosis, and rheumatoid arthritis.

The combination of HIV proteins Tat and Nef is chemotactic for CD4+ cells. Utilizing the capacity of Tat and Nef to modulate CD4+ cell trafficking and infiltration, the invention provides various treatment modes for individuals infected with HIV. The invention further provides treatment modes for other localized diseases by controlling CD4+ cell trafficking and infiltration. In particular, the invention provides methodology for promoting CD4+ cell chemotaxis to a localized site of infection as a means of augmenting the efficacy of extant chemotherapeutic methods. The invention further provides methodology for diverting CD4+ cell infiltration from a localized site where the presence of CD4+ cells is detrimental to the clinical outcome, by providing a composition comprising Tat and Nef at a distinct site, such as blood, within the individual where the accumulation of CD4+ cells is less detrimental.

The invention belongs to the field of biological medicine, and particularly relates to the application of CCL15 chemotactic factor in the preparation of follicular thyroid carcinoma (FTC) screening reagent. According to the application, a fresh thyroid follicular tumor tissue sample which is confirmed to be FTC is taken as a research object, and protein extraction, cell factor antibody chip detection, cell culture, cell chemotaxis experiment, antibody neutralization test and the like are carried out on the research object, so that the high expression of CCL15 in the FTC is verified, the infiltrating density of macrophage in the CCL15 high-expression group is remarkably larger than that of the macrophage in the CCL15 low-expression group, and the FTC cultural supernatant is capable of promoting the migration of Thp-1 mononuclear cell strain. Therefore, CCL15 can be taken as a potential biomarker and can be applied to the FTC screening reagent; furthermore, after the CCL15 is applied to the FTC screening reagent, experimental data and theoretical basis can be provided.

The aim of the invention is to provide a micro-fluidic chip and a cell chemotaxis movement research method. The micro-fluidic chip is characterized in that the micro-fluidic chip comprises a glass substrate, a first diaphragm and a second diaphragm; the first diaphragm is provided with culturing units; the second diaphragm is provided with micro-channels; a porous membrane exists between the culturing units of the first diaphragm and the micro-channels of the second diaphragm; the number of the culturing units of the first diaphragm is same to the number of the micro-channels of the second; and the micro-channels on the second diaphragm are respectively located above the culturing units of the first diaphragm. The micro-fluidic chip can be used in the research of the selective chemotaxis movement condition of cells in the micro-channels when the cells flow through the culturing units. The micro-fluidic chip likes a bionic model characterized in that a branching capilary goes through organs; and compared with the traditional cell chemotaxis research methods, the research method is a new method for researching the selective chemotaxis movement of the cells in the flowing process, and has important biomedical research values and economic values.

The invention discloses a CMKLR1 antagonistic polypeptide and its derivatives and applications, specifically related to the sequence of SEQ ID No.1‑24 and its derivatives, the derivatives of the binding peptide are CMKLR1 binding peptide amino acid side chain group, CMKLR1 The product obtained by conventional modification of the amino-terminal or carboxyl-terminal of the antagonistic polypeptide fragment, or the product obtained by linking a tag for polypeptide or protein detection or purification on the CMKLR1 antagonistic polypeptide; the binding peptide and its derivatives can bind CMKLR1 in vitro , by blocking the combination of chemerin / RvE1 and CMKLR1, it promotes the increase of cAMP concentration, inhibits the calcium influx caused by chemerin, inhibits the cell chemotaxis caused by chemerin, and can inhibit the proliferation of ovarian cancer cells and promote the apoptosis of ovarian cancer cells. Small molecule drugs provide effective treatments for female reproductive diseases such as ovarian cancer.