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Micro-fluidic chip and method for studying cell chemotaxis

A microfluidic chip and cell technology, applied in the biomedical field of microfluidic chip technology, can solve the problems of inability to intuitively observe the cell migration process, inability to apply, and difficulty in realizing indirect contact co-culture of cells, etc. Analyze time and cost, design flexibility, and create easy-to-make effects

Active Publication Date: 2018-04-13
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cells are planted in the upper chamber. Due to the permeability of the polycarbonate membrane, the components in the lower culture medium can affect the cells in the upper chamber, so that the influence of the components in the lower culture medium on cell growth and movement can be studied. This method cannot directly observe the migration process of cells
Other commonly used cell migration research methods include scratch experiments, etc. It is difficult to achieve indirect contact co-culture of cells and cannot be applied to this type of research

Method used

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  • Micro-fluidic chip and method for studying cell chemotaxis
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  • Micro-fluidic chip and method for studying cell chemotaxis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Indirect contact co-culture of cells

[0067] Such as figure 1 As shown, a microfluidic chip for studying cell chemotaxis, the chip sequentially includes a base layer 14, a cell culture layer 13, a cell culture layer 12, and a cell migration layer 11 from bottom to top. Sequentially aligned and sealed together; the cell migration layer 11 is provided with a group of connected structures formed by the communication area 15, the reservoir 16 and 17; the cell culture layer 12 has reservoirs 18 and 19 (each reservoir The pools are not connected to each other); the cell culture layer 13 has a liquid storage tank 110; the liquid storage tanks 18 and 16 correspond to the upper and lower positions, and the liquid storage tanks 110 and 19, 17 correspond to the upper and lower positions.

[0068] The base layer 14 is a glass slide, the cell migration layer 11 , the cell culture layers 12 and 13 are all PDMS films with a thickness of 1 mm, and the size of the film is 2 ...

Embodiment 2

[0074] Example 2 Migration of one cell under the action of cytokines secreted by another cell

[0075] Such as figure 2 As shown, a microfluidic chip for studying cell chemotaxis, the chip sequentially includes a base layer 24, a cell culture layer 23, a cell culture layer 22, and a cell migration layer 21 from bottom to top. Sequentially aligned and sealed together; the cell migration layer 21 is provided with a group of connected structures, which include sequentially connected reservoirs 27, connected regions 25, reservoirs 28, connected regions 26 and reservoirs 29; cell culture There are liquid reservoirs 210 / 211 / 212 (interconnected) on the layer 22; there is a liquid reservoir 213 on the cell culture layer 23;

[0076] The liquid storage tanks 213 correspond to the upper and lower positions of the liquid storage tanks 211 and 28; the liquid storage tanks 210 and 27, and the liquid storage tanks 212 and 29 correspond to the upper and lower positions respectively.

[00...

Embodiment 3

[0082]Example 3 Simultaneous research on cell migration of multiple groups on one chip

[0083] Such as image 3 As shown, a microfluidic chip for studying cell chemotaxis, the chip sequentially includes a base layer 35, a cell culture layer 34, a cell culture layer 33, a cell culture layer 32, and a cell migration layer 31 from bottom to top, each layer according to The sequence from bottom to top is aligned and sealed together; the cell migration layer 31 is provided with a set of communication structures, which include a fluid storage pool 38, a communication area 36, ​​a fluid storage pool 39, a communication area 37 and a fluid storage pool connected in sequence 310; there are reservoirs 311 / 312 / 313 on the cell culture layer 32 (the reservoirs are not connected to each other); there are reservoirs 314 / 315 on the cell culture layer 33 (the reservoirs are not connected to each other); cell culture Layer 34 has a fluid reservoir 316 thereon.

[0084] The liquid storage tan...

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Abstract

The invention relates to a micro-fluidic chip and a method for studying cell chemotaxis. The micro-fluidic chip is of a multi-layer structure and comprises a substrate layer, cell culture layers and acell migration layer, wherein the layers are sealed together in sequence; reservoirs for cell culture are arranged on the cell culture layers; different cells are cultured on different layers; the cell migration layer is provided with reservoirs and communicating areas; cell chemotaxis observation and study are implemented in the communicating areas. By adopting the micro-fluidic chip, indirect contact type coculture of multiple cells can be achieved, stable concentration gradients can be achieved, and cell chemotaxis under the action of cell factors is studied. The micro-fluidic chip has theadvantages of being flexible in design, simple to manufacture, simple and convenient to operate, low in use amount of cells and reagents, possible in real-time observation on cell migration process,and the like, and can be applied to various studies on cell indirect contact type coculture, cell chemotaxis, cell migration and the like.

Description

technical field [0001] The invention relates to the application of microfluidic chip technology in the field of biomedicine, and specifically provides a microfluidic chip for cell chemotaxis research and a cell chemotaxis research method based on the chip. Background technique [0002] The microfluidic chip technology that appeared in the early 1990s is a scientific technology for precise manipulation of biological and chemical samples at the micron scale. Fast, small and portable, high throughput, and integration of multiple operating units. The development of microfluidic chip technology not only provides a small portable laboratory on a chip (lab on a chip) for biochemical analysis, but also brings new opportunities for cell biology research. Since the size of the microchannel of the chip is equivalent to the size of the cell, and it is easy to regulate the microenvironment of the cell, the microfluidic chip has become a new technical platform for cell culture and resear...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12Q1/02
CPCC12M23/16C12M35/08G01N33/5029
Inventor 李雷刘静
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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