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Otology biological material

A biomaterial and ear technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of weak cell growth ability, less active ingredients to promote wound healing, and unfavorable tissue repair, etc. Forming the effect of promoting, flexible and diverse use methods, accelerated growth and functional recovery

Pending Publication Date: 2020-02-18
上海白衣缘生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] The purpose of the present invention is to solve the shortcomings and deficiencies of the existing otic biomaterials, such as weak ability to promote tissue regeneration in biomaterials (patch) or few active ingredients for promoting wound healing (such as hyaluronic acid HA), poor tissue induction ability ; The decellularization process is harsh, resulting in serious loss of active ingredients in the patch, damage to the ECM structure, and weak ability to induce cell growth; slow degradation of the patch, which is not conducive to tissue repair; for the technical problems of the aforementioned otic biomaterials; invention People revolve around the selection of the initial raw materials of the patch, the selection of the decellularization reagent, and the optimization of the process, starting from these key technical features; it simply and conveniently overcomes the shortcomings of the existing technical solutions, and cleverly solves the existing problems. problems in technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Embodiment one: (weaned piglet SIS)

[0070] Healthy weaned piglets were selected, and the submucosa of the small intestine was taken to prepare otic biopatch. The specific steps were as follows:

[0071] 1) Pre-treatment: take materials and clean them, select healthy three-yuan mixed piglets that have reached the weaning age and weight, take their small intestines, clean them and use them as young animal tissue raw materials; after in-depth understanding, the age of piglets at weaning is usually 21- -30 days, due to different seasons, the weaning age is different, it is shorter in summer and longer in winter; the weight is about 6 to 8 kg, and the piglets mainly eat breast milk before weaning, and the full price feed they consume (Some are called creep feed or piglet milk powder) are rare. The mucosal layer, muscular layer, serosal layer, lymph nodes, etc. of the small intestine and jejunum were removed by mechanical scraping, and cut into sections to obtain the small...

Embodiment 2

[0074] Embodiment two: (control group one, commercial meat pig)

[0075] The specific operation in the preparation method is basically the same as that of Example 1, the main difference being that the age and body weight of the pigs are different. In this example, the commercial meat pigs from the slaughterhouse were selected, with a body weight of nearly 100 kg, and the number of days of feeding reached 150 --about 160 days; other processing methods, steps, methods such as pretreatment (comprising pretreatment, disinfection, degreasing), decellularization, follow-up processing and use reagent, concentration and action time, are completely with embodiment one.

Embodiment 3

[0076] Embodiment three: (control group two, trypsin)

[0077] The specific operation in the preparation method is basically the same as that of Example 1, the main difference is that: the selection is the second link, the selection of the decellularization reagent; instead of using plant saponin, use conventional tests, such as trypsin, working concentration It is 0.05%, and the action time is the same, both are 30 minutes; it is just that the trypsin solution of the same concentration will not be soaked again, that is, the trypsin solution is only used once (30 minutes).

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Abstract

The invention relates to an otology biological material which can be used for repairing ear wounds including ear drum membrane injury. The material is prepared in the mode that young stock tissue suchas small intestinal submucosa of weaned piglets is used, and tender tissue of young stock is subjected to decellularization under the low-temperature condition through mild plant-derived reagents such as saponin. According to the obtained otology biological material, an ECM three-dimensional structure of the otology biological material is complete, the content of bioactive components is obviouslyhigher than that of an existing product, especially the content of hyaluronic acid is high, cell chemotaxis, growth and proliferation can be better promoted, and otology wound surface healing is facilitated; meanwhile, the otology biological material is easy to degrade and absorb and has an anti-infection effect; the defects of few active ingredients, much ECM structure damage, weak healing promoting capability and slow degradation of the existing product are overcome; and in addition, the dosage form of the otology biological material can be further made into different shapes such as powder,and thus use is more flexible, even simpler, more convenient and painless.

Description

technical field [0001] The invention belongs to the field of biological restoration materials, and in particular relates to an ear biological material. Background technique [0002] Although the human eardrum is very thin, its anatomical structure has three layers (tense part), the outer layer is the epithelial layer, which is continuous with the skin of the external auditory canal, and essentially belongs to the skin epithelial cells, the main components are collagen and skin cells, I, V Collagen types are arranged in layers and orderly, among which type Ⅰ collagen with uniform diameter and regular arrangement is an important factor to maintain the growth and arrangement of tympanic membrane epithelial cells. Then there is the middle layer, which is composed of radial and circular cilia, and has certain elasticity and tension. It is also collectively called the fibrous layer (radial on the outside and wheel-shaped on the inside). There is a small part above the eardrum with...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/54
CPCA61L27/3633A61L27/3641A61L27/3687A61L27/54A61L2430/14A61L2430/40
Inventor 韩韦红葛翠兰钱锵张国强
Owner 上海白衣缘生物工程有限公司
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