Biological patch for tympanic membrane repair and preparation method thereof

Abstract

The invention belongs to the field of otology repair materials, and relates to a biological patch for tympanic membrane repair and a preparation method thereof. The otology repair patch of the invention overcomes the shortcomings of the prior patch due to an improper decellularizing process and a complicated process, and causes serious loss of effective active ingredients in the ECM. The biological patch adopts the plant-derived saponin as a decellularizing reagent, which avoids the residue and toxicity of a chemical reagent, and the mild decellularizing reagent is highly targeted and mainly binds to the cell membrane lipid; the decellularizing reagent has little damage on an ECM stereoscopic three-dimensional structure and the active components thereof; and more biologically active components such as glycosaminoglycan (including hyaluronic acid) can be retained. The patch of the invention has a better ECM scaffold structure and more active components, and has stronger ability to induce cell chemotaxis and proliferation, is beneficial to faster and better repair of the tympanic membrane, and also has a certain anti-infective effect; at the same time, the method of the invention issimple and convenient to operate, and has no damage to the ECM.

Description

technical field [0001] The invention relates to the field of tympanic membrane repair materials in otology, in particular to a decellularized patch for tympanic membrane repair and a preparation method thereof. Background technique [0002] The adult tympanic membrane is about 9 mm long, 8 mm wide, and 0.1 mm thick; although the tympanic membrane is very thin, its anatomical structure has three layers (tense parts): the outer layer is the epithelial layer, which is continuous with the skin of the external auditory canal, and is essentially a Skin epithelial cells, the main components are collagen and skin cells; the middle layer is composed of radial and circular cilia, which has certain elasticity and tension, and is also collectively called the fibrous layer (radial on the outside and wheel-shaped on the inside); the inner layer is the mucous membrane The tympanic layer is connected with the tympanic membrane and is formed by its continuation. Type VI collagen forms the st...

AI Technical Summary

Problems solved by technology

[0026] The object of the present invention is to aim at the existing tympanic membrane biopatch derived from dermis, which has the disadvantages of complex process, slow degradation, and more elastin, less active ingredients, and weak inductive ability; Materials, the decellularization process is harsh, resulting in serious loss of active ingredients in the patch, damage to the ECM structure, and weak ability to induce cell growth, which cannot effectively meet the actual clinical needs; for some shortcomings and defects of the aforementioned otology patch; The inventor focused on the selection of patch raw materials, the selection of decellularization reagents, and the optimization of the process, starting from these key technical features; by reading a large number of relevant documents at home and abroad, combined with the profound academic attainments of the two returnee doctors of the company , as well as a solid theoretical foundation, coupled with the careful analysis and judgment of other members of the R&D team and years of work experience, the cutting-edge theory is closely combined with specific practical needs, which simply and conveniently overcomes the shortcomings of the existing tympanic membrane biological patch, and cleverly Solve existing technical problems

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