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Endogenous cell chemotaxis and chondrogenic differentiation induction bioactive scaffold and application thereof

A bioactive and endogenous technology, applied in the field of bioactive scaffolds that chemoattract endogenous cells and induce chondrogenic differentiation and its preparation, can solve the problems of unreported bioactive scaffolds and achieve the effect of improving the repair effect

Inactive Publication Date: 2019-02-19
陈元峰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, no bioactive scaffolds with endogenous cell chemotaxis and induction of chondrogenic differentiation have been reported at home and abroad.

Method used

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  • Endogenous cell chemotaxis and chondrogenic differentiation induction bioactive scaffold and application thereof
  • Endogenous cell chemotaxis and chondrogenic differentiation induction bioactive scaffold and application thereof
  • Endogenous cell chemotaxis and chondrogenic differentiation induction bioactive scaffold and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] An embodiment of the bioactive scaffold for chemotaxis of endogenous cells and induction of chondrogenic differentiation in the present invention comprises the following components: SDF-1, TGF-β, silk silk silkworm white and gelatin sponge; said SDF-1, The mass ratio of TGF-β to silk fibroin silkworm white is 1×10 -6 : 1×10 -5 :10.

[0041] The preparation method of the bioactive scaffold comprises the following steps:

[0042] (1) Weigh SDF-1, TGF-β and silk fibroin silkworm white in proportion, add water to dissolve, and prepare a protein mixture, so that the concentration of SDF-1 in the protein mixture is 1ng / ml, and the concentration of TGF-β The concentration is 10ng / ml, and the concentration of silk silk silk is 10mg / ml;

[0043] (2) Take a sterile dry gelatin sponge with a pore diameter of 600 μm, absorb the protein mixture prepared in step (1) onto the porous support to saturation, and then vacuum freeze-dry at -80°C for 1 day to obtain the Bioactive scaffo...

Embodiment 2

[0046] An embodiment of the bioactive scaffold for chemotaxis of endogenous cells and induction of chondrogenic differentiation in the present invention comprises the following components: SDF-1, TGF-β, silk silk silkworm white and gelatin sponge; said SDF-1, The mass ratio of TGF-β to silk fibroin silkworm white was 1.5×10 -4 : 5×10 -4 :80.

[0047]The preparation method of the bioactive scaffold comprises the following steps:

[0048] (1) Weigh SDF-1, TGF-β and silk fibroin silkworm white in proportion, add water to dissolve, prepare protein mixture, make the concentration of SDF-1 in the protein mixture be 150ng / ml, TGF-β The concentration is 500ng / ml, and the concentration of silk silk silk is 80mg / ml;

[0049] (2) Take a sterile dry sodium alginate sponge with a pore diameter of 50 μm, absorb the protein mixture prepared in step (1) onto the porous support until saturated, and then vacuum freeze-dry at -10°C for 7 days to obtain The bioactive scaffold.

[0050] The m...

Embodiment 3

[0052] An embodiment of the bioactive scaffold with endogenous cell chemotaxis and chondrogenic differentiation functions of the present invention, comprising the following components: SDF-1, TGF-β, silk silk silk silk and chitosan porous scaffold; The mass ratio of SDF-1, TGF-β and silk fibroin silkworm white is 3×10 -5 :0.001:200.

[0053] The preparation method of the bioactive scaffold comprises the following steps:

[0054] (1) Weigh SDF-1, TGF-β and silk fibroin silkworm white in proportion, add water to dissolve, and prepare protein mixture, make the concentration of SDF-1 in the protein mixture be 30ng / ml, the concentration of TGF-β The concentration is 1000ng / ml, and the concentration of silk silk silk is 200mg / ml;

[0055] (2) Take a sterile dry chitosan porous scaffold with a pore diameter of 300 μm, absorb the protein mixture prepared in step (1) onto the porous scaffold to saturation, and then vacuum freeze-dry at -40°C for 3 days, that is, Obtain the bioactive...

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Abstract

The invention discloses an endogenous cell chemotaxis and chondrogenic differentiation induction bioactive scaffold and a preparation method and application thereof in preparation of cartilage defectrepair materials. The bioactive scaffold comprises SDF-1, TGF-beta, fibroin and a porous scaffold. The bioactive scaffold takes natural fibroin as a medium, and by a freeze drying technique, a gene cell derived factor (SDF-1) with a chemotaxis effect, a transforming growth factor-beta (TFG-beta) and fibroin are fixed into the porous scaffold through crystal phase transition. When the scaffold is transplanted to a cartilage injury / or defect area, the chemotaxis factor SDF-1 can be released for a long time to enable chemotaxis migration of autologous endogenous cells to the injury area while chondrogenic differentiation can be induced by TGF-beta in long-term controlled release, and accordingly endogenous cell attraction and implementation of effective cartilage injury repair are realized.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a bioactive scaffold for chemoattracting endogenous cells and inducing chondrogenic differentiation, a preparation method and application thereof. Background technique [0002] Osteoarthritis (OA) is the most common degenerative joint disease. Its main pathological features include: cartilage degradation, irregular ossification of subchondral bone and its secondary inflammation. The main clinical features are: joint pain, stiffness, limited range of motion, etc. At present, clinical drug therapy has little effect on alleviating the progression of the disease. In the advanced stage of the disease, most patients with osteoarthritis have to undergo joint replacement surgery, but the risk of surgery is high and postoperative complications are prone to occur, and the joint The service life of the replacement prosthesis makes the long-term curative effect poor. ...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/54A61L27/56
CPCA61L27/26A61L27/54A61L27/56A61L2300/252A61L2300/412A61L2430/06C08L89/00C08L5/08C08L5/04
Inventor 陈元峰吴婷婷黄树森查振刚
Owner 陈元峰
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