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Rapid propagation method for botrypus lanuginosus suspension cell

A technology of suspended cells and shaded ferns, applied in the field of plants, achieves the effects of fast growth, simple operation and low energy consumption

Inactive Publication Date: 2014-12-10
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, P. tomentosa has not been effectively developed and utilized. The acquisition method is wild collection, and the main reproduction method is natural reproduction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the leaves of Pseudomonas tomentosa, soak them in bleaching powder for 3 minutes, remove surface stains and hairs with a brush, wash them with running water for 30 minutes, treat them with mercuric chloride on an ultra-clean workbench for 15 minutes, rinse them with sterile water 5-7 times, and disinfect them Grind the treated leaves of F. tomentosa in a glass homogenizer to make them broken, and then filter the broken tissue with a stainless steel mesh with a pore size of 200 mesh to remove large cell clusters or tissue pieces. The screened broken tissue is inserted into the culture medium Formula is B 5 Suspension cell culture in +NAA0.1mg / L+6-BA1mg / l+200mg / L yeast extract medium, add sucrose 30g / L, pH5-6, light 2000lx, temperature 23℃, inoculate 80mL per 250ml Erlenmeyer flask Liquid, using horizontal oscillation, speed 100-150r / min, amplitude 2-4cm, culture suspension cell liquid for a period of time to remove dead cells and cell debris, insert 2mg / L cerium nit...

Embodiment 2

[0011] Take the leaves of Pseudomonas tomentosa, soak them in bleaching powder for 3 minutes, remove surface stains and hairs with a brush, wash them with running water for 30 minutes, treat them with mercuric chloride on an ultra-clean workbench for 15 minutes, rinse them with sterile water 5-7 times, and disinfect them Grind the treated leaves of F. tomentosa in a glass homogenizer to make them broken, and then filter the broken tissue with a stainless steel mesh with a pore size of 200 mesh to remove large cell clusters or tissue pieces. The screened broken tissue is inserted into the culture medium Formula is B 5 Suspension cell culture in +NAA0.2mg / L+6-BA2mg / l+200mg / L yeast extract medium, add sucrose 30g / L, pH5-6, light 2000lx, temperature 23℃, inoculate 80mL per 250ml Erlenmeyer flask Liquid, using horizontal oscillation, speed 100-150r / min, amplitude 2-4cm, culture suspension cell liquid for a period of time to remove dead cells and cell debris, insert 5mg / L cerium nit...

Embodiment 3

[0013] Take the leaves of Pseudomonas tomentosa, soak them in bleaching powder for 3 minutes, remove surface stains and hairs with a brush, wash them with running water for 30 minutes, treat them with mercuric chloride on an ultra-clean workbench for 15 minutes, rinse them with sterile water 5-7 times, and disinfect them Grind the treated leaves of F. tomentosa in a glass homogenizer to make them broken, and then filter the broken tissue with a stainless steel mesh with a pore size of 200 mesh to remove large cell clusters or tissue pieces. The screened broken tissue is inserted into the culture medium Formula is B 5 Suspension cell culture in +NAA0.1mg / L+6-BA2mg / l+200mg / L yeast extract medium, add sucrose 30g / L, pH5-6, light 2000lx, temperature 23℃, inoculate 80mL per 250ml Erlenmeyer flask Liquid, using horizontal oscillation, speed 100-150r / min, amplitude 2-4cm, culture suspension cell liquid for a period of time to remove dead cells and cell debris, insert 5mg / L cerium nit...

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PUM

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Abstract

The invention researches a rapid propagation method for a botrypus lanuginosus suspension cell. The method comprises the steps of acquiring a sterile leaf, smashing tissues, acquiring a cell suspension liquid, propagating the suspension cell and the like. By the method, a large quantity of botrypus lanuginosus cells are acquired rapidly, the operations are simple, the propagation rate of the cells is high, the growth cycle is short, the botrypus lanuginosus cells can be provided infinitely, and the development and the utilization of the botrypus lanuginosus are promoted.

Description

technical field [0001] The invention relates to the cultivation of the suspension cells of the fern pseudoshadum under the condition of tissue culture, and belongs to the technical field of plants. Background technique [0002] Pseudomonas tomentosa, Botrypus lanuginosus (Hook. et Grev.) Holub (1973), Pteridaceae, produced in Yunnan, China, Lijiang, Diqing and Nujiang prefectures; altitude 1500-2700 meters. Whole herbal medicine, bitter in taste, cool in nature. It has the effects of clearing heat and detoxifying, relieving cough and reducing phlegm, nourishing and strengthening. Treatment of pulmonary tuberculosis, swollen lymph nodes, breast cancer, pharyngitis, snake bites, postpartum physical weakness and other diseases. This species is also a common medicine of the Yi people. It is very effective in treating poisonous snake bites, cough due to lung heat, and hemoptysis due to tuberculosis. At present, P. tomentosa has not been effectively developed and utilized. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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