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Detection kit for detecting content of beta-hydroxybutyrate in serum by adopting stable enzymatic method

A detection kit, hydroxybutyric acid technology, applied in biological testing, color/spectral property measurement, material testing, etc., can solve the problems of weak anti-interference ability, poor stability of reagents, etc. The effect of high, accelerated reaction time

Inactive Publication Date: 2014-12-10
上海睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is much more sensitive than kinetics, but the reagents produced by this method have poor stability and low anti-interference ability

Method used

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  • Detection kit for detecting content of beta-hydroxybutyrate in serum by adopting stable enzymatic method
  • Detection kit for detecting content of beta-hydroxybutyrate in serum by adopting stable enzymatic method
  • Detection kit for detecting content of beta-hydroxybutyrate in serum by adopting stable enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The composition of the assay kit is as follows:

[0030] 1. Reagent R1 is:

[0031]

[0032] 2. Reagent R2 is:

[0033]

[0034] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0035] 3. Preparation of bovine serum matrix calibrator:

[0036] With the processed bovine serum, add bovine serum albumin (BSA) 1%, NaN 3 0.9%, Tween-201% to obtain calibrator dilutions. Dissolve the solution of β-hydroxybutyric acid in the prepared serum matrix to prepare a standard substance containing β-hydroxybutyric acid with a concentration of 1 mmol / L.

[0037] 4. The reagent detection ratio is 3:1

[0038] The β-HB detection kit described in this example is applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is...

Embodiment 2

[0043] The composition of the assay kit is as follows:

[0044] 1. Reagent R1 is:

[0045]

[0046] 2. Reagent R2 is:

[0047]

[0048] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0049] 3. Preparation of bovine serum matrix calibrator:

[0050] Add BSA2% to the processed bovine serum, NaN 3 0.2%, Tween-205% to get the calibrator dilution. Dissolve the solution of β-hydroxybutyric acid in the prepared serum matrix to prepare a standard substance containing β-hydroxybutyric acid with a concentration of 1 mmol / L.

[0051] 4. The reagent detection ratio is 3:1

[0052] The β-HB detection kit described in this example is applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the dosage of reagents R1...

Embodiment 3

[0054] The composition of the assay kit is as follows:

[0055] 1. Reagent R1 is:

[0056]

[0057] 2. Reagent R2 is:

[0058]

[0059] Components can be added sequentially at room temperature, added simultaneously, or individually packaged and reconstituted immediately prior to testing.

[0060] 3. Preparation of bovine serum matrix calibrator:

[0061] Add BSA3% to the processed bovine serum, NaN 3 2.2%, Tween-2010% to obtain calibrator dilutions. A solution containing β-hydroxybutyric acid dissolved in the prepared serum matrix was used to prepare a standard substance containing β-hydroxybutyric acid at a concentration of 1 mmol / L.

[0062] 4. The reagent detection ratio is 3:1

[0063] The β-HB detection kit described in this example is applicable to various types of automatic biochemical analyzers. Taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: Two-point endpoint method, that is, the dosage...

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Abstract

The invention discloses a detection kit for detecting the content of beta-hydroxybutyrate in serum by adopting a stable enzymatic method. The detection kit comprises a reagent R1, a reagent R2 and a Beta-HB-containing bovine serum matrix calibrator, wherein the reagent R1 contains 10-500mmol / L buffer solution with the pH value of 7.0-8.0, 1-50mmol / L LNAD<+>, 1-10mmol / L magnesium chloride, 0.1-20mmol / L nitrotetrazolium blue chloride, 1-100mmol / L stabilizer and 0.1-2% of a preservative; the reagent R2 contains 10-500mmol / L buffer solution with the pH value of 7.0-8.0, 0.5-5KU / L beta-hydroxybutyrate, 0.5-10KU / L diaphorase, 1-45mmol / L saccharose, 10-30mmol / L EDTA, 1-100mmol / L stabilizer and 0.1-2% of a preservative. The reagents have the excellent stability and the long preservation time.

Description

technical field [0001] The invention specifically relates to a detection kit for detecting the content of β-hydroxybutyric acid (β-HB) in serum by a stable enzyme method. Background technique [0002] Ketone bodies are the products of fatty acid catabolism and consist of three components: β-hydroxybutyrate, acetoacetate, and acetone. Among them, β-hydroxybutyric acid is the main component, accounting for about 78% of the total ketone bodies. The liver produces ketone bodies, and extrahepatic tissues can use ketone bodies as energy sources. Under normal circumstances, there are only a small amount of ketone bodies in the blood; but in In some cases, carbohydrate metabolism is impaired, body fat mobilization is enhanced, and the amount of ketone bodies produced by the liver exceeds the ability of extrahepatic tissues to utilize ketone bodies, causing the accumulation of ketone bodies in the blood, which can lead to ketoacidosis. Therefore, it is extremely important to detect ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N33/96
Inventor 李伟奇陈瑛房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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