Preparation method of spray-dried pig blood cells

A technology for porcine hemoglobin powder and hemoglobin, which is applied in the field of preparation of porcine hemoglobin powder, can solve the problems such as the need for improvement of decolorization effect and large ash content, and achieve the effects of improving immunity, low ash content and improving sensory properties.

Active Publication Date: 2015-01-07
浙江索纳克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the ash content of the product prepared by this method is r...

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  • Preparation method of spray-dried pig blood cells
  • Preparation method of spray-dried pig blood cells
  • Preparation method of spray-dried pig blood cells

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Preparation of porcine blood hemoglobin solution: after blood collection from qualified pigs, add disodium citrate, sodium bicarbonate and tea polyphenols or apple polyphenols in a mass ratio of 1:5:0.03, wherein disodium citrate and pig blood The weight ratio is 2:1000. After mixing evenly, the first disc centrifugation is performed at 25°C for 6 minutes to obtain the first supernatant layer and the first sediment layer. Ionized water, the first ultrasonic treatment at 2500W ultrasonic power for 60s to obtain porcine hemoglobin solution;

[0055] Pre-treatment: use nitrogen bubbling deoxygenation to carry out the first physical deoxygenation and preliminary decolorization of the porcine hemoglobin solution, then adjust the pH value of the deoxygenated porcine hemoglobin solution to 7, and then perform the second ultrasonic treatment under the ultrasonic power of 800W 90s to obtain the pretreatment solution;

[0056] Segmented enzymatic decolorization: raise the temper...

Embodiment 2

[0065] Preparation of porcine blood hemoglobin solution: after blood collection from qualified pigs, add disodium citrate, sodium bicarbonate and tea polyphenols or apple polyphenols in a mass ratio of 1:2:0.01, wherein disodium citrate and pig blood The weight ratio of the mixture is 1:1000. After mixing evenly, centrifuge for the first time at 15°C for 3 minutes to obtain the first supernatant layer and the first precipitate layer. Separate the first precipitate and add it to the first precipitate. Ionized water, the first ultrasonic treatment at 1800W ultrasonic power for 30s to obtain porcine hemoglobin solution;

[0066] Pre-treatment: Use nitrogen bubbling deoxygenation to perform the first physical deoxygenation and preliminary decolorization of the porcine hemoglobin solution, then adjust the pH value of the deoxygenated porcine hemoglobin solution to 6, and then perform the second ultrasonic treatment at 600W ultrasonic power 50s to obtain the pretreatment solution; ...

Embodiment 3

[0074] The preparation of porcine hemoglobin solution is specifically to collect blood from pigs that pass the inspection, and then add disodium citrate, sodium bicarbonate and plant phenol extracted from natural plants in a mass ratio of 1:3:0.03, wherein disodium citrate and The weight ratio of pig blood is 1.5:1000. After mixing evenly, centrifuge for the first time at 20°C for 4 minutes to obtain the first supernatant layer and the first sediment layer. Separate the first precipitate and pour it into the first precipitate. 1.8-2 times the volume of deionized water was added, and the first ultrasonic treatment was performed for 50 seconds under the ultrasonic power of 2000W to obtain a porcine hemoglobin solution.

[0075] Pre-treatment: use nitrogen bubbling deoxygenation to carry out the first physical deoxygenation and preliminary decolorization of the porcine hemoglobin solution, then adjust the pH value of the deoxygenated porcine hemoglobin solution to 6.5, and then pe...

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Abstract

The invention relates to a pig blood utilization method, particularly to a preparation method of spray-dried pig blood cells. The method comprises steps as follows: pig blood hemoglobin solution preparation: mixing disodium citrate, sodium bicarbonate and plant phenol extracted from natural plants in the mass ratio of 1:(2-5):(0.01-0.03), adding the mixture in pig blood, performing centrifugal separation to obtain a first supernatant layer and a first sediment layer, performing separation to obtain a first sediment, and performing ultrasonic treatment for the first time; pretreatment: performing physical deoxidation, adjusting the pH value, and performing ultrasonic treatment for the second time; segmented enzymolysis decoloration; activated carbon decoloration: adding activated carbon accounting for 1-2% of the weight for decoloration treatment at the temperature of 25-35 DEG C for 5-10 min; aftertreatment: adjusting the pH value, performing centrifugal separation to obtain a second supernatant layer and a second sediment layer, and taking the second supernatant layer for ultrafiltration, concentration and separation to obtain a second concentrated solution; spray drying: performing spray drying on the second concentrated solution to obtain milky peptide spray-dried pig blood cells. The method is good in decoloration effect and can be used for preparing products which are high in protein content and low in ash content.

Description

technical field [0001] The invention relates to a pig blood utilization method, in particular to a preparation method of pig blood globulin powder. Background technique [0002] The protein content of red blood cells in pig blood is as high as 38%, accounting for more than 75% of the total protein in whole blood, of which more than 92% is hemoglobin, so pig blood cells are an excellent source of animal protein. Made into blood cell powder for sale. But because the blood cell powder contains heme, the blood cell powder is black and red, and has a heavy bloody smell, which is unacceptable to the sense organs, and its economic added value is not high. [0003] Heme is in the form of porphyrin iron combined with four globins to form hemoglobin, which exists in red blood cells. In the food industry, heme can replace the chromogen nitrate and artificial pigments in meat products, and the use of heme can reduce the carcinogenic effect of nitrite. In the pharmaceutical industry, ...

Claims

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Application Information

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IPC IPC(8): A23J1/06A23J3/34A23J3/12
Inventor 刘观洲邓自伟沙欢李宁丰
Owner 浙江索纳克生物科技有限公司
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