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Construction method of model for detecting guanosine yield by using guanosine fermentation liquid ultraviolet fingerprint spectra

A kind of technology of fingerprint spectrum and construction method, applied in the field of biological fermentation detection

Active Publication Date: 2015-01-21
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Construction method of model for detecting guanosine yield by using guanosine fermentation liquid ultraviolet fingerprint spectra
  • Construction method of model for detecting guanosine yield by using guanosine fermentation liquid ultraviolet fingerprint spectra
  • Construction method of model for detecting guanosine yield by using guanosine fermentation liquid ultraviolet fingerprint spectra

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Embodiment 1

[0140] The construction method of the guanosine production model using the UV fingerprint of guanosine fermentation liquid, using the ultraviolet spectrophotometer as a detection tool to construct the UV fingerprint of guanosine, so as to construct the mathematical model of the relationship between the UV fingerprint and the amount of guanosine produced, and realize it through the model The on-line detection of guanosine output, concrete operation comprises the following steps:

[0141] Step (1), model establishment:

[0142] A. Guanosine fermentation:

[0143] 1) Activation of strains

[0144] Take a single colony on the plate and inoculate it on the activated slant, and culture it at a constant temperature of 36°C for 12 hours;

[0145] 2) Seed cultivation

[0146] Connect two rings of well-grown activated slant strains into a 250mL shaker flask with 15mL seed medium, seal with 8 layers of gauze, place on a reciprocating shaker, stroke 76mm, speed 108r / min, shake at 36°C ...

Embodiment 2

[0159] The construction method of the guanosine production model using the UV fingerprint of guanosine fermentation liquid, using the ultraviolet spectrophotometer as a detection tool to construct the UV fingerprint of guanosine, so as to construct the mathematical model of the relationship between the UV fingerprint and the amount of guanosine produced, and realize it through the model The on-line detection of guanosine output, concrete operation comprises the following steps:

[0160] Step (1), model establishment:

[0161] A. Guanosine fermentation:

[0162] 1) Activation of strains

[0163] Take a single colony on the plate and inoculate it on the activated slant, and culture it at a constant temperature of 38°C for 14 hours;

[0164] 2) Seed cultivation

[0165] Connect two rings of well-grown activated slant strains into a 250mL shaker flask with 15mL seed medium, seal with 8 layers of gauze, place on a reciprocating shaker with a stroke of 76mm, a speed of 108r / min, ...

Embodiment 3

[0178] The construction method of the guanosine production model using the UV fingerprint of guanosine fermentation liquid, using the ultraviolet spectrophotometer as a detection tool to construct the UV fingerprint of guanosine, so as to construct the mathematical model of the relationship between the UV fingerprint and the amount of guanosine produced, and realize it through the model The on-line detection of guanosine output, concrete operation comprises the following steps:

[0179] Step (1), model establishment:

[0180] A. Guanosine fermentation:

[0181] 1) Activation of strains

[0182] Take a single colony on the plate and inoculate it on the activated slant, and culture it at a constant temperature of 37°C for 13 hours;

[0183] 2) Seed cultivation

[0184] Connect two rings of well-grown activated slant strains into a 250mL shaker flask with 15mL of seed medium, seal with 8 layers of gauze, place on a reciprocating shaker, stroke 76mm, speed 108r / min, shake at 36...

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Abstract

The invention relates to a construction method of a model for detecting the guanosine yield by using guanosine fermentation liquid ultraviolet fingerprint spectra, and belongs to the bio-fermentation detection technical field. An ultraviolet spectrophotometer is used as a detection tool to construct the guanosine ultraviolet fingerprint spectra, and thus the mathematical model of the relationship between the ultraviolet fingerprint spectra and the guanosine production amount is constructed; online detection of the guanosine yield is achieved through the model, and specific steps comprise model establishment, model verification and model application. A new efficient, accurate and fast method is provided for determination of the guanosine yield, and the method is simple and fast to operate, and strongly promotes innovation of a guanosine fermentation detection technology.

Description

technical field [0001] The invention belongs to the field of biological fermentation detection, and in particular relates to a method for constructing a model for detecting guanosine yield by using the ultraviolet fingerprint of guanosine fermentation broth. Background technique [0002] The biological fermentation process is a real-time, nonlinear, multi-variable input-output and random dynamic process. During the fermentation process, the composition of the fermentation broth is complex, and its components and contents are time-varying. During the fermentation process, as the properties of the fermentation broth change, its ultraviolet absorption The spectrum will also show corresponding changes, and the ultraviolet absorption spectrum of the fermentation broth in different periods will be brought together to form an ultraviolet spectrum, which is called the ultraviolet spectrum fingerprint of the guanosine fermentation broth. This kind of fingerprint is different from the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G06F19/00
Inventor 杨天佑田静张明霞常景玲
Owner HENAN INST OF SCI & TECH
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