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Construction method of detection model of guanosine yield by ultraviolet fingerprint of guanosine fermentation broth

A kind of technology of fingerprint spectrum and construction method, applied in the field of biological fermentation detection

Active Publication Date: 2017-04-26
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Construction method of detection model of guanosine yield by ultraviolet fingerprint of guanosine fermentation broth
  • Construction method of detection model of guanosine yield by ultraviolet fingerprint of guanosine fermentation broth
  • Construction method of detection model of guanosine yield by ultraviolet fingerprint of guanosine fermentation broth

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Embodiment 1

[0140] The construction method of using the UV fingerprint of guanosine fermentation broth to detect the production of guanosine, using the UV spectrophotometer as the detection tool to construct the UV fingerprint of guanosine, so as to construct the mathematical model of the relationship between the UV fingerprint and the production of guanosine. On-line detection of guanosine production, the specific operation includes the following steps:

[0141] Step (1), model establishment:

[0142] A. Guanosine fermentation:

[0143] 1) Strain activation

[0144] The single colony on the plate was streaked and inoculated on the activation slant, and it was incubated at a constant temperature of 36 °C for 12 h;

[0145] 2) Seed cultivation

[0146] Connect two rings of well-grown activated slanted strains into a 250 mL shake flask containing 15 mL of seed medium, seal with 8 layers of gauze, and place on a reciprocating shaker with a stroke of 76 mm, a speed of 108 r / min, and shakin...

Embodiment 2

[0159] The construction method of using the UV fingerprint of guanosine fermentation broth to detect the production of guanosine, using the UV spectrophotometer as the detection tool to construct the UV fingerprint of guanosine, so as to construct the mathematical model of the relationship between the UV fingerprint and the production of guanosine. On-line detection of guanosine production, the specific operation includes the following steps:

[0160] Step (1), model establishment:

[0161] A. Guanosine fermentation:

[0162] 1) Strain activation

[0163] The single colony on the plate was streaked and inoculated on the activation slant, and it was incubated at a constant temperature of 38°C for 14h;

[0164] 2) Seed cultivation

[0165] Connect two rings of well-grown activated slanted strains into a 250mL shaker flask containing 15mL of seed medium, seal with 8 layers of gauze, and place on a reciprocating shaker with a stroke of 76mm, a speed of 108r / min, and shaking at ...

Embodiment 3

[0178] The construction method of using the UV fingerprint of guanosine fermentation broth to detect the production of guanosine, using the UV spectrophotometer as the detection tool to construct the UV fingerprint of guanosine, so as to construct the mathematical model of the relationship between the UV fingerprint and the production of guanosine. On-line detection of guanosine production, the specific operation includes the following steps:

[0179] Step (1), model establishment:

[0180] A. Guanosine fermentation:

[0181] 1) Strain activation

[0182] The single colony on the plate was streaked and inoculated on the activation slant, and it was incubated at 37°C for 13h;

[0183] 2) Seed cultivation

[0184] Connect two rings of well-grown activated slanted strains into a 250 mL shake flask with 15 mL of seed medium, seal with 8 layers of gauze, and place it on a reciprocating shaker with a stroke of 76 mm, a speed of 108 r / min, and shaking at 36.5 °C for 16 h. , get t...

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Abstract

The invention relates to a construction method of a model for detecting the guanosine yield by using guanosine fermentation liquid ultraviolet fingerprint spectra, and belongs to the bio-fermentation detection technical field. An ultraviolet spectrophotometer is used as a detection tool to construct the guanosine ultraviolet fingerprint spectra, and thus the mathematical model of the relationship between the ultraviolet fingerprint spectra and the guanosine production amount is constructed; online detection of the guanosine yield is achieved through the model, and specific steps comprise model establishment, model verification and model application. A new efficient, accurate and fast method is provided for determination of the guanosine yield, and the method is simple and fast to operate, and strongly promotes innovation of a guanosine fermentation detection technology.

Description

technical field [0001] The invention belongs to the field of biological fermentation detection, and in particular relates to a method for constructing a guanosine yield detection model by using an ultraviolet fingerprint of a guanosine fermentation broth. Background technique [0002] The biological fermentation process is a dynamic process with real-time, nonlinear, multi-variable input and output and randomness. During the fermentation process, the composition of the fermentation broth is complex, and the components and contents are time-varying. During the fermentation process, as the properties of the fermentation broth change, its UV absorption The spectrum will also show corresponding changes. The ultraviolet absorption spectra of the fermentation broth in different periods are collected together to form an ultraviolet spectrum, which is called the ultraviolet spectral fingerprint of the guanosine fermentation broth. The difference between this fingerprint and the UV s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G06F19/00
Inventor 杨天佑田静张明霞常景玲
Owner HENAN INST OF SCI & TECH
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