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Application of rhubarb monomer in preparation of inhibiting Streptococcus suis or intervening Streptococcus suis biofilm

A Streptococcus suis, biofilm technology, applied in antibacterial drugs, medical preparations containing active ingredients, organic active ingredients, etc., to achieve the effect of reducing mRNA expression

Active Publication Date: 2017-05-03
李艳华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that rhubarb has antibacterial and anti-inflammatory, diarrhea, liver protection, hemostasis, blood lipid lowering, parasite inhibition, diuresis, tumor suppression, anti-inflammatory analgesic and central antipyretic effects, etc. A report of monomer inhibiting Streptococcus suis or interfering with Streptococcus suis biofilm

Method used

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  • Application of rhubarb monomer in preparation of inhibiting Streptococcus suis or intervening Streptococcus suis biofilm
  • Application of rhubarb monomer in preparation of inhibiting Streptococcus suis or intervening Streptococcus suis biofilm
  • Application of rhubarb monomer in preparation of inhibiting Streptococcus suis or intervening Streptococcus suis biofilm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 The intervening effect of rhubarb monomer on Streptococcus suis biofilm

[0044] 1. Experimental method

[0045] 1.1 Establishment of S.suis biofilm model

[0046] Streptococcus suis strains were inoculated on THB agar plates and cultured overnight at 37°C. A single colony was inoculated in THB medium, and cultured at 37°C for 12h-16h. Dilute the bacterial solution with a turbidity of 0.5 McFarland units to about 1.0×10 6 cfu mL -1 , take 200 μL and inoculate in the Calgary biofilm device, the wells containing only THB medium are the blank control group, mix well, cover well, and incubate on a shaker at 37°C and 50rmp for 72h. The Calgary biofilm device was removed, free radicals were discarded with a pipette and washed gently three times with sterile PBS (pH 7.2) to remove free bacteria. The pellicle bacteria still attached to the Calgary biofilm device were fixed with 200 μL methanol for 30 min. After the fixative was discarded and allowed to dry na...

Embodiment 2

[0071] Example 2 Effect of emodin on biofilm signal molecules and virulence genes of Streptococcus suis

[0072] 1. Experimental method

[0073] 1.1 Real time-PCR detection of mRNA expression of virulence factors under the biofilm

[0074] 1.1.1 Total RNA extraction

[0075] With reference to the method of Example 1, culture and collect the Streptococcus suis thalline treated with emodin at 1 / 2, 1 / 4, 1 / 8MIC, and use the unmedicated biofilm growth control as a positive control, centrifuge at 10000g for 5min, and collect the bacteria In a 1.5mL EP tube, wash the bacteria 2-3 times with DEPC-treated water. After resuspending the bacteria in 100 μL of TE containing lysozyme, place at room temperature for 10 minutes, add 1 mL of Trizol to each tube, mix well, and let stand at room temperature for 5 minutes; add 0.2 mL of chloroform, shake and mix for 15 seconds, let stand for 2 minutes; centrifuge at 4°C, 12000 g , 15min; take the supernatant in another EP tube, add 0.5mL isopro...

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Abstract

The invention discloses a use of a rheum officinale monomer in the preparation of medicines for inhibiting Streptococcus suis or intervening a Streptococcus suis biofilm, and belongs to the field of new medicinal uses of the rheum officinale monomer. A biofilm model is established, and crystal violet staining and colony count detection results show that the rheum officinale monomer has an inhibition effect on the formation of the Streptococcus suis biofilm. A fluorescent quantitative PCR detection result shows the influence of emodin on the expression level of mRNA of virulence genes and signaling molecules in the Streptococcus suis biofilm is a dose effect relationship, and the expression of the mRNA of respective virulence genes and the signaling molecules significantly decreases. The rheum officinale monomer can definitely inhibit the Streptococcus suis or intervene the Streptococcus suis biofilm, can be applied to prepare medicines for inhibiting the Streptococcus suis, intervening the formation of the Streptococcus suis biofilm or intervening the expression of the virulence genes or signaling molecules in the Streptococcus suis biofilm. A new treatment way is provided for Streptococcus suis biofilm related infections.

Description

technical field [0001] The invention relates to new medical applications of rhubarb monomers, in particular to applications of rhubarb monomers in preparing medicines for inhibiting Streptococcus suis or interfering with Streptococcus suis biofilm, and belongs to the field of new medical applications of rhubarb monomers. Background technique [0002] Type Ⅱ Streptococcus suis (S.suis) is an important zoonotic pathogen, which can cause suppurative meningitis, sepsis, endocarditis, pneumonia and arthritis in pigs. After S. suis forms a biofilm (biofilm, BF), it is difficult to completely remove the infection site, resulting in persistent infection. Biofilm refers to the three-dimensional space structure formed by single or multiple bacteria adsorbing on the surface of non-biological substances or the surface of the mucous membrane of the body and the surface of implanted biomedical materials and secreting extracellular polysaccharides in order to adapt to the surrounding envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/122A61K31/192A61P31/04
CPCA61K31/122A61K31/192
Inventor 李艳华韩强陈俭清闫清波刘冰赵玉林黄全勇周永辉
Owner 李艳华
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