Method for producing photoactive phenylethanol through cell catalysis
A technology of phenylethyl alcohol and cell biology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of difficult chemical synthesis and high cost
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Embodiment 1
[0017] Penicillium wet ATCC 60254 cells were prepared by conventional methods and used as catalysts for biocatalytic reactions.
[0018] The method for making the ramie gauze that has adsorbed the substrate is that the substrate acetophenone is absorbed by the ramie gauze with a size of 0.5cm×0.5cm to obtain the ramie gauze that has adsorbed the substrate, and the amount of the substrate and the ramie gauze is adjusted so that the substrate and the ramie gauze are absorbed. The mass ratio of ramie gauze is 0.29. Used ramie gauze is common ramie gauze, after buying from the market, be cut into 0.5cm * 0.5cm fritter, sterilize, aseptic preservation stand-by, other embodiment ramie gauze processes identically.
[0019] Add phosphate buffer in the 15L bottom aeration stirred reaction tank, the filling coefficient is 0.6, and pH is 5.8, adds the ramie gauze that has adsorbed the substrate acetophenone, makes the substrate acetophenone addition be 80g / L, corn steep liquor (with Dry...
Embodiment 2
[0021] Penicillium wet ATCC 60254 cells were prepared by conventional methods and used as catalysts for biocatalytic reactions.
[0022] The method for making the ramie gauze that has adsorbed the substrate is that the substrate acetophenone is absorbed by the ramie gauze with a size of 0.5cm×0.5cm to obtain the ramie gauze that has adsorbed the substrate, and the amount of the substrate and the ramie gauze is adjusted so that the substrate and the ramie gauze are absorbed. The mass ratio of ramie gauze was 0.31. Used ramie gauze is common ramie gauze, after buying from the market, be cut into 0.5cm * 0.5cm fritter, sterilize, aseptic preservation stand-by, other embodiment ramie gauze processes identically.
[0023] Add phosphate buffer solution to the 100L bottom ventilated stirring reaction tank, the filling coefficient is 0.6, the pH is 5.8, add the ramie gauze that has adsorbed the substrate acetophenone, so that the substrate acetophenone is added in an amount of 80-90 g...
Embodiment 3
[0025] Penicillium wet ATCC 60254 cells were prepared by conventional methods and used as catalysts for biocatalytic reactions.
[0026] The method for making the ramie gauze that has adsorbed the substrate is that the substrate acetophenone is absorbed by the ramie gauze with a size of 0.5cm×0.5cm to obtain the ramie gauze that has adsorbed the substrate, and the amount of the substrate and the ramie gauze is adjusted so that the substrate and the ramie gauze are absorbed. The mass ratio of ramie gauze is 0.30. Used ramie gauze is common ramie gauze, after buying from the market, be cut into 0.5cm * 0.5cm fritter, sterilize, aseptic preservation stand-by, other embodiment ramie gauze processes identically.
[0027]Add phosphate buffered saline in the 500L bottom ventilated stirring reaction tank, and filling coefficient is 0.6, and pH is 5.8, adds the ramie gauze that has adsorbed substrate acetophenone, makes substrate acetophenone addition be 85 g / L, and corn steep liquor (...
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