Method for preparing chitosan oligose from fly grub shells

A technology of chitosan and oligosaccharides, which is applied in the field of preparation of chitosan oligosaccharides, can solve the problems of limited and full utilization, and achieve the effects of high product quality, strong operability, good moisture absorption and moisture retention

Inactive Publication Date: 2015-02-18
XIAN MIYI BIOTECH
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the presence of acetyl groups in the structure of chitin, strong hydrogen bonds are formed between molecules, so it is almost insoluble in water, dilute

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Grind 100g of fly maggot shells, place them in a reflux device with 800mL of 40% sodium hydroxide solution, and reflux at 118°C for 14 hours. After degrading with hydrogen peroxide for 5 hours, suction filtration, the filtrate was rotary evaporated and precipitated with ethanol, the precipitate was vacuum dried and pulverized to obtain 48.29 g of soluble chitosan oligosaccharides.

Embodiment 2

[0015] Grind 100g of fly maggot shells, place them in a reflux device with 800mL of 40% sodium hydroxide solution, and reflux at 118°C for 12 hours. After degrading with hydrogen peroxide for 4 hours, suction filtration, the filtrate was rotary evaporated and then precipitated with ethanol. The precipitate was vacuum dried and pulverized to obtain 42.56 g of soluble chitosan oligosaccharides.

Embodiment 3

[0016] Embodiment 3: Moisture absorption and moisture retention experiment

[0017] The samples were dried at 105°C for 4 hours and cooled. Weigh 0.5g in a small beaker, place in a desiccator with a saturated ammonium hydrogen phosphate aqueous solution to maintain a relative humidity of 93% for moisture absorption, and transfer it to a desiccator with a saturated calcium chloride aqueous solution to maintain a relative humidity of 32% after 4 days , weighed after a certain period of time, and calculate the moisture absorption rate and moisture retention rate.

[0018] Through moisture absorption and moisture retention experiments, it is found that under the condition of humidity 93%, the hygroscopicity of chitosan oligosaccharide prepared by the present invention can reach 31%; under the condition of humidity 32%, its moisture retention can reach about 8%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for preparing chitosan oligose from fly grub shells, and belongs to fields of food additives, in particular to oligose and a preparation method thereof. The method comprises the following steps: grinding the fly grub shells, laying the fly grub shells and a sodium hydroxide solution into a reflux device, so as to be subjected to heating reflux treatment, filtering the fly grub shells through a gauze, washing to be neutral, insolating the fly grub shells in the sun, so as to be decolored, degrading with hydrogen peroxide under the neutral condition, performing suction filtration, performing rotary evaporation on a filtrate, enabling the filtrate to be precipitated with ethyl alcohol, performing vacuum drying and crushing on the precipitate, and then obtaining the soluble chitosan oligose. The method ensures that the yield of the chitosan oligose is 48.29 percent, and has the characteristics of simple technology, high operability, high product yield, high product quality and the like.

Description

technical field [0001] The invention relates to a method for preparing chitosan oligosaccharides from fly maggot shells, and belongs to the field of food additives, especially oligosaccharides and preparation methods thereof. Background technique [0002] Due to the presence of acetyl groups in the structure of chitin, strong hydrogen bonds are formed between molecules, so it is almost insoluble in water, dilute acids and bases and common organic solvents, which limits its full utilization. Chitosan is currently the most commercially used Most chitin deacetylation derivatives. Low-molecular-weight chitosan has the advantages of good solubility and easy absorption by the human body, and has important application prospects in the food and pharmaceutical industries. At present, chitosan is mainly derived from shrimp and crab shells. Because shrimp and crab shells contain a lot of calcium, it causes great trouble for extraction and consumes a lot of acid. Studies have shown tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07H5/06C07H1/00C08B37/08
Inventor 李勇刘文斌
Owner XIAN MIYI BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap