Molecular marking method for indicating common premunition of sheep
A technology of molecular markers and disease resistance, applied in the field of animal molecular genetics, can solve the problems of high price, low result accuracy, and low cost, and achieve the effects of increasing sheep survival rate, reducing morbidity, and low cost
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[0031] Examples: The following examples describe the present invention in more detail:
[0032] 1. Detection and analysis of the 9th exon mutation site of IDO1 gene in sheep
[0033] 1. Sample collection
[0034] Use a disposable syringe to draw about 1 mL of blood from the jugular vein of the sheep, and inject it into a 1.5 mL centrifuge tube that is autoclaved and contains about 200 μL of 2% sterile EDTA (Ethylene diamine tetraacetic acid, EDTA) anticoagulant, and shake it gently. , Record the sheep number, store at -20℃ for later use.
[0035] 2. Drugs and enzymes
[0036] Tris (Tris), Sigma Chemicals Co; Tris saturated phenol, EDTA-Na 2 Beijing Dingguo Biotechnology Development Center; Proteinase K (Proteinase K), MERCK Co; dNTP (dATP; dTTP; dCTP; dGTP), Taq enzyme (supporting 10×Taq buffer, 25mmol / L Mg) 2+ ), Dalian Bao Biological Company; Agarose (Agarose H), pUC19DNA / Msp (Hpa Ⅱ) Marker, Sangon Bioengineering (Shanghai) Co., Ltd.; Restriction Enzyme Nhe Ⅰ, Fermentas (MBI) Company...
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