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A kind of pine needle extract and application thereof

A technology of pine needle extract and slash pine, applied in application, biocide, animal repellent, etc., can solve the problems of little effect, residue, large environmental pollution, etc., to improve water quality, excellent inhibition and kill moss Effect

Active Publication Date: 2017-02-22
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of physical methods is time-consuming and labor-intensive; the use of chemical methods such as proteochlorine powder and copper sulfate has great environmental pollution, residues, and is unsafe; the use of biological methods such as probiotics is costly and has little effect

Method used

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  • A kind of pine needle extract and application thereof
  • A kind of pine needle extract and application thereof
  • A kind of pine needle extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Inhibitory effect of slash pine needle extracts of different molecular weights on moss.

[0024] (1) Prepare stock solution

[0025] Take fresh slash pine needles and cut them into 2-3 cm long with scissors, then crush them with a high-speed pulverizer, take 100 g of crushed pine needles, add tap water at a ratio of 1:10 for extraction for 24 hours, then filter with nylon gauze to remove the residue, and use A vacuum pump is used for suction filtration to obtain a sample as a stock solution.

[0026] (2) 1KD molecular weight cut-off dialysis

[0027] Use a 1KD molecular weight cut-off dialysis bag to take 200mL of the tap water extract (stock solution) of Pinus sclerosus (to prevent the concentration of the dialysis fluid from being too low) and place it in a 1000mL beaker for dialysis. After dialysis for 12 hours, take out the dialysis fluid (components less than 1KD) and place it at 4°C Store in the refrigerator. Then add 500mL of distilled water to conti...

Embodiment 2

[0035] Example 2 Inhibitory effect of pine needle extracts eluted with different concentrations of NaCl on moss.

[0036] (1) Pretreatment of resin

[0037] Take a 250mL ion exchange column packed with AB-8 resin, fill the column with about 2 / 3 volume of the resin, then add ethanol equivalent to 0.4-0.5 times the volume of the loaded resin into the adsorption column, and soak the resin for 24 hours. After washing the ethanol with deionized water, soak it with 3% hydrochloric acid for 2 hours, wash the resin with deionized water until it is neutral; then soak it with 3% sodium hydroxide solution for 2 hours, wash it with deionized water until it is neutral.

[0038] (2) Preparation of penetration solution

[0039] Take fresh slash pine needles and cut them into 2-3 cm long with scissors, then crush them with a high-speed pulverizer, take 100 g of crushed pine needles, add tap water at a ratio of 1:10 for extraction for 24 hours, then filter with nylon gauze to remove the resid...

Embodiment 3

[0061] Example 3 Inhibitory effect of pine needle extracts eluted with different concentrations of ethanol on moss.

[0062] (1) Pretreatment of resin

[0063] Take a 250mL ion exchange column packed with AB-8 resin, fill the column with about 2 / 3 volume of the resin, then add ethanol equivalent to 0.4-0.5 times the volume of the filled resin into the adsorption column, and soak the resin for 24 hours. After washing the ethanol with deionized water, soak it with 3% hydrochloric acid for 2 hours, wash the resin with deionized water until it is neutral; then soak it with 3% sodium hydroxide solution for 2 hours, wash it with deionized water until it is neutral.

[0064] (2) Preparation of penetration solution

[0065] Take fresh slash pine needles and cut them into 2-3 cm long with scissors, then crush them with a high-speed pulverizer, take 100 g of crushed pine needles, add tap water at a ratio of 1:10 for extraction for 24 hours, then filter with nylon gauze to remove the re...

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Abstract

The invention discloses slash pine needle extract. A preparation method of the slash pine needle extract comprises the following steps of shearing fresh slash pine needles by scissors to obtain slash pine needle sections with length of 2-3cm, crushing the sections by a high-speed crusher, adding water into the crushed pine needle sections according to a ratio of the crushed pine needle sections to the water of 1: (10-12), carrying out extraction for 24-26h, carrying out filtration by nylon gauze to remove residue, carrying out suction filtration by a vacuum pump to obtain a pine needle extract stock solution and carrying out dialysis purification to obtain the slash pine needle extract with different molecular weight ranges. The slash pine needle extract can be used for effectively inhibiting and killing moss in aquatic product culture, can improve water quality, has no toxicity, no harm, residue and pollution on the environment and other aquatic organisms, realizes prevention and control of moss in aquatic product culture and develops a novel application value and prospect of pine needles.

Description

technical field [0001] The invention belongs to the field of aquaculture and relates to the technical field of moss killing in river crab culture ponds, in particular to a slash pine needle extract and its application in the field of moss killing. Background technique [0002] Slash pine is an evergreen tree of Pinaceae, which has the characteristics of strong adaptability, fast growth in the early stage and high rosin yield. Studies have shown that slash pine needles contain a lot of volatile oils, pigments, cellulose, amino acids, flavonoids and other compounds, among which there are as many as 18 kinds of free amino acids, rich in 8 kinds of amino acids necessary for human beings, and vitamins are several times higher than ordinary fruits and vegetables times or even dozens of times, in addition to calcium, phosphorus, iron, manganese and other trace elements. Slash pine needle extract also has strong medicinal value, such as anti-inflammation, lowering blood fat, loweri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/06A01P13/00
Inventor 赵明珍王会聪张萍凡军民陆静万启俊
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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