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A combined detection kit for AFP, gp73 and pivka-ii

A detection kit, -73GP73 technology, applied in the direction of biological testing, material inspection products, etc., can solve problems such as false negatives, achieve high specificity, high sensitivity, fast and easy operation

Active Publication Date: 2016-01-13
ZYBIO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the use of serum AFP as a marker of HCC has a certain degree of false positives and false negatives

Method used

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  • A combined detection kit for AFP, gp73 and pivka-ii
  • A combined detection kit for AFP, gp73 and pivka-ii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Preparation of the test paper card of the present invention:

[0049] 1) Use pretreatment buffer to pretreat the gold label pad. The pretreatment buffer is: stachyose 2g / L, alum 0.5g / L, sodium fructose diphosphate 1.5g / L, sodium hexametaphosphate 0.3g / L, glycine 1.88g / L aqueous solution, pH=7.4, the specific pretreatment steps are: soak the gold-labeled pad in the pretreatment solution for 2h, take it out and dry it at 37℃; then use fluorescent microspheres to label the fetus Protein AFP antibody, Golgi glycoprotein-73GP73 antibody, and protein PIVKA-II antibody solution induced by vitamin K deficiency or antagonist-II were sprayed with their corresponding pretreated gold label pads to prepare AFP containing alpha-fetoprotein. Antibody, Golgi glycoprotein-73GP73 antibody and vitamin K deficiency or antagonist-II induced protein PIVKA-II antibody gold label pad; the mass ratio of fluorescent microspheres to antibody in the solution is 5:1, and the concentration of the solu...

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Abstract

The invention relates to the field of biological detection, in particular to a detection kit for quantitatively detecting AFP (Alpha Fetal Protein), Golgi Protein-73GP73 and PIVKA (Protein Induced Vy Vitamin K Absence)-II or an antagonist-II as well as a preparation method and application of the detection kit. The detection kit comprises an AFP detection test paper card, a Golgi Protein-73GP73 detection test paper card and a PIVKA-II detection test paper card which are independent of one another, wherein each detection test paper card comprises a bottom plate, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorbing pad, wherein the sample pad, the gold-labeled pad, the nitrocellulose membrane and the water absorbing pad are positioned on the surface of the bottom plate and are arranged from the sample adding end in sequence. The kit provided by the invention has the benefits that the AFP, the GP73 and the PIVKA-II are firstly detected through a fluorescent microsphere immune chromatography, and both the sensitivity and the specificity are realized; the kit has the advantages of high quickness, simplicity and convenience in operation, accurate results, good economy and applicability and the like.

Description

Technical field [0001] The invention relates to the field of biological detection, in particular to a combined detection kit of AFP, GP73, and PIVKA-II, and a preparation method and application thereof. Background technique [0002] Alphafetoprotein (AFP) is a glycoprotein synthesized by the liver and yolk sac in the early stage of fetal development. As the fetus develops and ages after birth, the blood AFP level gradually decreases. The reference interval for adult serum AFP concentration is 1-20μg / L. AFP is a sensitive and specific marker of hepatocellular carcinoma (HCC). The serum AFP concentration exceeds 400μg / L for 4 weeks or 200-400μg / L for more than 5 weeks. It is highly suspected of hepatocellular carcinoma; at the same time, the serum AFP level is also It can be used to judge the size of liver cancer, evaluate the curative effect of liver cancer and judge the prognosis. However, according to statistics, about 20%-30% of patients with hepatocellular carcinoma have low...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 潘能科
Owner ZYBIO INC