A kind of method for extracting anthocyanin from raspberry

Abstract

The invention relates to a method for extracting black chokeberry anthocyanin. By using black chokeberries as raw materials, the method comprises the following steps: firstly, pulping and homogenating the black chokeberries and then carrying out ultrasonic-assisted extraction on anthocyanin by using an ethanol-acetic acid-aqueous solvent; after extraction, carrying out suction filtration in vacuum to remove filter residues; performing rotary evaporation on a crude extract for removing ethanol to obtain an anthocyanin concentrated liquid; removing impurities by using distilled water; then, eluting anthocyanin by using an ethanol solution; after ultrahigh pressure treatment on the eluant, extracting the impurities by using ethyl acetate; after performing standing and layering, collecting an aqueous phase; freezing and drying a black chokeberry anthocyanin water phase solution in vacuum to obtain anthocyanin powder. The method provided by the invention is simple in process operation, pollution-free and high in extraction amount, the purity of anthocyanin obtained is high, and the nutritional value of black chokeberries is given full play to, and moreover, macroporous resin is convenient and quick to activate and can be repeatedly used, so that the cost is lowered.

Description

technical field

[0001] The invention relates to a method for extracting anthocyanins from bulimia. Background technique

[0002] The old berry has high nutritional health value and economic value, and the anthocyanin content of its fruit is the highest among known plants. Anthocyanins are natural and effective antioxidants, and have the effect of protecting eyesight and treating diabetes and cardiovascular diseases. The content of anthocyanins in berry is relatively high, and most of them exist in pomace, and it has good physiological functions, so it is a good source of anthocyanins. There is currently no published scientific method for extracting anthocyanins from berry. Contents of the invention

[0003] The purpose of the present invention is to provide a method for extracting anthocyanins from berry, which has high extraction amount, high purity, less impurities and no pollution.

[0004] The preparation method of the old berry anthocyanin provided by the invention...

Examples

Embodiment 1

[0008] Take 1 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 3L of ethanol, 0.5L of acetic acid, and 1.5L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain anthocyanins Crude extract, the crude extract was rotary evaporated to no alcohol smell. Use 2000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 4L, and then use 95% ethanol for elution, the elution volume is 10L. Obtain the eluate of anthocyanins from the old berry. The anthocyanin eluate from the old berry was treated by ultra-high pressure equipment at 700Mpa and 40°C for 30 minutes, and then the ethanol was removed by vacuum rotary evaporation, extracted with 1.5L ethyl acetate, and the organic phase was removed after standing fo...

Embodiment 2

[0010] Take 2 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 6L of ethanol, 1L of acetic acid, and 3L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain crude anthocyanins. solution, and the crude extract was rotary evaporated until it had no alcohol smell. Use 4000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 12L, and then use 95% ethanol for elution, the elution volume is 24L. Obtain the eluate of anthocyanins from the old berry. The anthocyanin eluate from the old berry was treated by ultra-high pressure equipment at 700Mpa and 40°C for 30 minutes, then the ethanol was removed by vacuum rotary evaporation, extracted with 9.5L ethyl acetate, and the organic phase was removed after st...

Embodiment 3

[0012] Take 5 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 15L of ethanol, 2.5L of acetic acid, and 7.5L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain anthocyanins Crude extract, the crude extract was rotary evaporated to no alcohol smell. Use 10000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 20L, and then use 85% ethanol for elution, the elution volume is 50L. Obtain the eluate of anthocyanins from the old berry. Treat the anthocyanin eluent of the old berry with ultra-high pressure equipment at 700Mpa and 45°C for 30 minutes, then remove the ethanol by vacuum rotary evaporation, extract with 10L ethyl acetate, remove the organic phase after standing for stratification, and co...

technical field

[0001] The invention relates to a method for extracting anthocyanins from bulimia. Background technique

[0002] The old berry has high nutritional health value and economic value, and the anthocyanin content of its fruit is the highest among known plants. Anthocyanins are natural and effective antioxidants, and have the effect of protecting eyesight and treating diabetes and cardiovascular diseases. The content of anthocyanins in berry is relatively high, and most of them exist in pomace, and it has good physiological functions, so it is a good source of anthocyanins. There is currently no published scientific method for extracting anthocyanins from berry. Contents of the invention

[0003] The purpose of the present invention is to provide a method for extracting anthocyanins from berry, which has high extraction amount, high purity, less impurities and no pollution.

[0004] The preparation method of the old berry anthocyanin provided by the invention...

Examples

Embodiment 1

[0008] Take 1 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 3L of ethanol, 0.5L of acetic acid, and 1.5L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain anthocyanins Crude extract, the crude extract was rotary evaporated to no alcohol smell. Use 2000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 4L, and then use 95% ethanol for elution, the elution volume is 10L. Obtain the eluate of anthocyanins from the old berry. The anthocyanin eluate from the old berry was treated by ultra-high pressure equipment at 700Mpa and 40°C for 30 minutes, and then the ethanol was removed by vacuum rotary evaporation, extracted with 1.5L ethyl acetate, and the organic phase was removed after standing fo...

Embodiment 2

[0010] Take 2 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 6L of ethanol, 1L of acetic acid, and 3L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain crude anthocyanins. solution, and the crude extract was rotary evaporated until it had no alcohol smell. Use 4000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 12L, and then use 95% ethanol for elution, the elution volume is 24L. Obtain the eluate of anthocyanins from the old berry. The anthocyanin eluate from the old berry was treated by ultra-high pressure equipment at 700Mpa and 40°C for 30 minutes, then the ethanol was removed by vacuum rotary evaporation, extracted with 9.5L ethyl acetate, and the organic phase was removed after st...

Embodiment 3

[0012] Take 5 kg of washed fresh berry fruit and beat it into a homogenate with a homogenizer. Take 15L of ethanol, 2.5L of acetic acid, and 7.5L of water and mix them to obtain the extractant. After fully mixing the berry homogenate with the extractant, perform ultrasonic extraction at 45°C for 90 minutes, and then vacuum filter the extract to obtain anthocyanins Crude extract, the crude extract was rotary evaporated to no alcohol smell. Use 10000mL of XAD7 macroporous resin for adsorption, the flow rate is controlled at 0.5BV / h, first use distilled water for elution, the elution volume is 20L, and then use 85% ethanol for elution, the elution volume is 50L. Obtain the eluate of anthocyanins from the old berry. Treat the anthocyanin eluent of the old berry with ultra-high pressure equipment at 700Mpa and 45°C for 30 minutes, then remove the ethanol by vacuum rotary evaporation, extract with 10L ethyl acetate, remove the organic phase after standing for stratification, and co...