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Detection method and detection kit for goat LMCD1 gene mononucleotide polymorphism sites

A single nucleotide polymorphism and polymorphic site technology, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of unreported correlation of polymorphic growth traits, etc., and achieve low cost , high precision effect

Inactive Publication Date: 2015-03-25
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

The research on the polymorphism of goat LMCD1 gene and its relationship with growth traits has not been reported so far

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  • Detection method and detection kit for goat LMCD1 gene mononucleotide polymorphism sites
  • Detection method and detection kit for goat LMCD1 gene mononucleotide polymorphism sites
  • Detection method and detection kit for goat LMCD1 gene mononucleotide polymorphism sites

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with the drawings and embodiments.

[0036] The present invention first designs primers based on the conservative sequence of the LMCD1 gene, uses the genomic DNA of four goat populations as templates to perform PCR amplification, mixes the PCR products, purifies them, and sequence them. Then, perform sequencing map analysis and sequence comparison to screen out SNP sites; secondly, perform PCR-SSCP detection of polymorphic sites in the population to be tested; finally, perform population genetic statistical analysis based on the genotypes detected in the population Correlation analysis with body size traits was used to screen out molecular markers closely related to goat body size traits. The present invention will be described in detail below, which is an explanation rather than a limitation of the present invention.

[0037] (1) Amplification of partial DNA sequence of goat LMCD1 gene and detection of p...

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Abstract

The invention discloses a detection method and detection kit for goat LMCD1 gene mononucleotide polymorphism sites. The method comprises the following steps: by using to-be-detected goat genome DNA (deoxyribonucleic acid) of containing LMCD1 gene as a template, carrying out PCR (polymerase chain reaction) amplification on the goat LMCD1 gene, and carrying out SSCP (single strand conformation polymorphism) polymorphism detection. The gene polymorphism sites comprise the following base polymorphisms: the 681st site of the goat LMCD1 gene is A or G; and the 2825th site of the goat LMCD1 gene is C or T. The invention provides a simple quick low-cost high-precision detection method, which can be conveniently applied to screening and detecting of genetic markers closely related to goat growth traits at the DNA level and can be used for assisted selection and molecular breeding of goat.

Description

Technical field [0001] The present invention relates to the detection of gene single nucleotide polymorphism (SNP), in particular to the detection of goat LMCD1 gene single nucleotide polymorphism sites. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the replacement of a single nucleotide (A / T / C / G) in the genomic DNA sequence, which is mainly caused by the conversion or transversion of a single base . SNPs with conversion variants accounted for about 2 / 3, and other SNPs were at similar levels. The cytosine of CpG dinucleotide is the most prone to mutation in the genome, most of which are methylated and can be spontaneously de-amino to form thymine. [0003] According to the influence on genetic traits, gene polymorphism can be divided into two kinds: one is synonymous mutation polymorphism, that is, the change of the coding sequence caused by SNP does not affect the amino acid sequence in the translated protein, and the mut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/158
Inventor 陈宏房兴堂刘宣宣张春雷金晶王艳红
Owner XUZHOU NORMAL UNIVERSITY
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