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Induction method capable of promoting accumulation of tanshinone in hairy roots of salvia miltiorrhiza

A hairy root, tanshinone technology, applied in the induction field capable of promoting the accumulation of tanshinone in the hairy root of Salvia miltiorrhiza

Active Publication Date: 2015-04-01
ZHEJIANG SCI-TECH UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, there is currently no report on the use of cerium nitrate to promote the accumulation of tanshinone in the hairy roots of Salvia miltiorrhiza

Method used

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  • Induction method capable of promoting accumulation of tanshinone in hairy roots of salvia miltiorrhiza
  • Induction method capable of promoting accumulation of tanshinone in hairy roots of salvia miltiorrhiza
  • Induction method capable of promoting accumulation of tanshinone in hairy roots of salvia miltiorrhiza

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Embodiment 1

[0030] Embodiment 1, a kind of induction method that can promote the accumulation of tanshinone (cryptanshinone and dihydrotanshinone I) in the hairy root of Salvia miltiorrhiza miltiorrhiza, carries out the following steps successively:

[0031] 1), initial cultivation of salvia miltiorrhiza hairy roots:

[0032] In an ultra-clean bench, weigh 0.2 g of the hairy root of Salvia miltiorrhiza and put it into 50 ml of 6,7-V medium (100 ml Erlenmeyer flask, pH: 5.8), shaker at 110 rpm, and culture in the dark at 25°C for 18 days .

[0033] 2), the continued cultivation of salvia miltiorrhiza root:

[0034] After the hairy roots of Salvia miltiorrhiza were cultured for 18 days, cerium nitrate was added so that the final concentration of cerium nitrate in the 6,7-V medium was 50 μM; culture was induced for 6 days at 110 rpm and 25° C. in the dark.

[0035] After 6 days of induction, the hairy roots were collected, rinsed with distilled water, blotted dry with clean paper, and fina...

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Abstract

The invention discloses an induction method capable of promoting accumulation of tanshinone in hairy roots of salvia miltiorrhiza. The method comprises the following steps: (1) performing primary culture on hairy roots of salvia miltiorrhiza, namely inoculating 0.15-0.2g of hairy roots of salvia miltiorrhiza in every 50mL of 6,7-V culture medium, and performing darkness culture under the culture conditions of 100-150rpm and 24-26 DEG C for 17-19 days; and (2) performing further culture on the hairy roots of salvia miltiorrhiza, namely adding cerous nitrate till the final concentration of the cerous nitrate in the 6,7-V culture medium is 45-55 micromole / liter, and performing darkness culture (induction culture) under the culture conditions of 100-150rpm and 24-26 DEG C for 5-7 days. With the adoption of the method disclosed by the invention, accumulation of tanshinone (comprising cryptotanshinone and dihydrotanshinone I) in hairy roots of salvia miltiorrhiza can be promoted.

Description

technical field [0001] The invention relates to an induction method capable of promoting the accumulation of cryptotanshinone and dihydrotanshinone I in the hairy root of Salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza (Salvia miltiorrhiza) is the root and rhizome of Salvia miltiorrhiza, which has the effects of promoting blood circulation and removing blood stasis, clearing heat and calming nerves, nourishing blood and nourishing qi. Tanshinone is one of the main active ingredients of Danshen, mainly including cryptotanshinone and dihydrotanshinone I. Due to its outstanding performance and non-toxic side effects in the treatment of cardiovascular diseases, the application and research of Danshen are becoming more and more extensive. [0003] Currently, the hairy root of Salvia miltiorrhiza can be cultured in 6,7-V medium. Generally, 0.15-0.2 g of fresh hairy roots are weighed and inoculated into 50 mL of 6,7-V medium for dark culture. [0004] The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁宗锁杨东风韩名宇郭万里杨宗岐金伟波刘岩
Owner ZHEJIANG SCI-TECH UNIV
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