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A kind of corn enzyme that improves reducing sugar content and its application

A corn enzyme and sugar content technology, applied in the application, animal feed, animal feed and other directions, can solve the problems of insufficient secretion of digestive enzymes, decreased appetite of young animals, growth stagnation, etc., to improve in vitro digestibility, low cost, source of wide range of effects

Active Publication Date: 2017-06-20
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the immature development of the digestive system of piglets, insufficient secretion of digestive enzymes, and low efficiency of digestion and utilization will cause a series of problems such as decreased appetite, diarrhea, and growth stagnation in young animals.
Most of the methods of extrusion and fine crushing are used in production to improve the utilization efficiency of corn, but these treatments only improve the physical properties of corn, and cannot fundamentally improve the carbohydrate decomposition level of corn, which prompts us Find ways to better engineer maize for uptake by young animals

Method used

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  • A kind of corn enzyme that improves reducing sugar content and its application
  • A kind of corn enzyme that improves reducing sugar content and its application
  • A kind of corn enzyme that improves reducing sugar content and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Adjust the ratio of corn and water, and control the moisture content of the material to be 40%; the control group only adds water, and the test group-1, on the basis of the control group, is inoculated with the glucoamylase component of the present invention at a mass ratio of 0.5%. Said corn ferment, test group-2 is on the basis of control group, by 0.5% mass ratio, inoculates the corn ferment of the present invention that does not contain Bacillus amyloliquefaciens component, and test group-3 is on the basis of control group, According to the mass ratio of 0.5%, the corn enzyme of the present invention is inoculated. 3 repetitions per set. After the materials are mixed evenly, they are placed in a double-layer sealed bag and fermented anaerobically at 37°C for 48 hours. The content of reducing sugar was determined by 3,5-dinitrosalicylic acid colorimetric method. The apparent metabolizable energy (AME) and true metabolizable energy (TME) of maize are determined by m...

Embodiment 2

[0031] Regulate the ratio of corn and water, control the material moisture content to be 40%; Regulate different inoculation proportions according to mass ratio: 0.1%, 0.3%, 0.5%, 0.7%, 1.0%, inoculate corn enzyme of the present invention, examine inoculation proportion Effect on reducing sugar content of corn. 3 repetitions per set. After the materials are mixed evenly, they are placed in a double-layer sealed bag and fermented anaerobically at 37°C for 48 hours. The content of reducing sugar was determined by 3,5-dinitrosalicylic acid colorimetric method.

[0032] figure 1 Show the reducing sugar content of corn fermentation under different inoculation ratios of corn ferment of the present invention. The results of this test show that when the inoculation ratio is lower than 0.5%, the reducing sugar content gradually increases with the inoculation ratio, and the highest increase reaches 111%; when the inoculation ratio is higher than 0.5%, the reducing sugar content incre...

Embodiment 3

[0034]Adjust the ratio of corn and water, control the water content of the material to be 30%, 40%, 50%, 60% respectively, and inoculate the corn enzyme of the present invention at a mass ratio of 0.5%. 3 repetitions per set. After the materials are mixed evenly, they are placed in a double-layer sealed bag and fermented anaerobically at 37°C for 48h. The content of reducing sugar was determined by 3,5-dinitrosalicylic acid colorimetric method.

[0035] figure 2 Show the corn fermentation reducing sugar content of corn ferment of the present invention under different moisture. The results of this experiment showed that when the moisture content of corn increased from 30% to 50%, the reducing sugar content in corn increased gradually, up to 46.43±2.32mg / g, and when the moisture content of corn reached 60%, the reducing sugar content decreased instead. Water is the main medium of fermentation and one of the key factors to determine the success or failure of fermentation. If...

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Abstract

The invention provides a corn enzyme with the effect of reducing sugar content increasing, and applications thereof. The corn enzyme comprises Bacillus amyloliquefaciens, Bacillus pumilus with the preservation number of CGMCC 9161, Bacillus coagulans, Lactobacillus rhamnosus, saccharifying enzyme, xylanase, cellulase, acid protease, neutral protease, C6H5O7(NH43), K2HPO4, MgSO4.7H20, and MnSO4. According to the present invention, the corn enzyme is inoculated in corn according to a mass ratio of 0.5%, the obtained material is placed in a double-layer sealed bag and is subjected to constant temperature anaerobic fermentation, the moisture content is adjusted to 50%, the fermentation temperature is controlled at 37 DEG C, and the fermentation time is controlled to 48 h, such that the reducing sugar content in the corn can be significantly improved, the in vitro corn digestibility can be increased, the total amount of probiotics can be increased, and the application of the feed micro-ecological additives or feed enzyme preparations can be reduced to a certain extent.

Description

technical field [0001] The invention belongs to the field of biological enzymolysis and fermentation, and relates to a corn enzyme formula, in particular to a corn enzyme for increasing reducing sugar content and its application. Background technique [0002] Corn has good palatability, relatively balanced amino acids, and a wide planting area. It is the most important grain source for livestock breeding. Corn is the main source of energy for weaned piglets, accounting for more than 60% of the total weight of the diet. However, due to the immature development of the digestive system of piglets, insufficient secretion of digestive enzymes, and low efficiency of digestion and utilization will cause a series of problems such as decreased appetite, diarrhea, and growth stagnation in young animals. Most of the methods of extrusion and fine crushing are used in production to improve the utilization efficiency of corn, but these treatments only improve the physical properties of c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/18A23K10/14A23K50/30A23K50/60
Inventor 李慧芬王文博马成
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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