Method for extracting docosahexenoic acid and eicosapentaenoic acid from seriola quinqueradiata

A technology of docosahexaenoic acid and eicosapentaenoic acid, which is applied in the separation/purification of carboxylic acid compounds, bulk chemical production, organic chemistry, etc., and can solve the problems of slow speed and low conversion amount. Achieve the effect of low raw material cost, stable properties and high extraction rate

Active Publication Date: 2015-04-01
杜丽红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

EPA belongs to the omega-3 series of polyunsaturated fatty acids. Although linolenic acid can be converted into EPA in the human body, the speed of this reaction in the human

Method used

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  • Method for extracting docosahexenoic acid and eicosapentaenoic acid from seriola quinqueradiata
  • Method for extracting docosahexenoic acid and eicosapentaenoic acid from seriola quinqueradiata
  • Method for extracting docosahexenoic acid and eicosapentaenoic acid from seriola quinqueradiata

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Freeze-dry the yellowtail fish, pulverize it, pass it through an 80-mesh sieve after grinding, sieve it twice, and put it in a desiccator for use;

[0024] (2) Weigh 50kg of processed yellowtail fish meal, put it on a supercritical carbon dioxide fluid extraction apparatus, use yellowtail fish meal as raw material, add entrainer in the ratio of entrainer: yellowtail fish meal=1:1~1:4 according to the weight ratio, Fill the kettle, seal it;

[0025] (3) Adjust the extraction pressure within the range of 18-30Mpa, and the extraction temperature between 38-54°C, and perform supercritical carbon dioxide fluid extraction for 3 hours;

[0026] (4) Adjust the collection pressure in the range of 24~36Mpa, and the collection temperature is between 40~60°C to separate and obtain the extract;

[0027] (5) Among them, the flow rate of carbon dioxide is 2ml / g.

Embodiment 2

[0028] The content determination of DHA and EPA in the extract of embodiment 2

[0029] The DHA and EPA content of the extract obtained in the experiment in the present invention, according to the "Determination of eicosapentaenoic acid and docosahexaenoic acid in food" in "Standards of the People's Republic of China" (GB / T5009.168- 2003) method for detection. Specific steps are as follows:

[0030] 1. Preparation of sample solution:

[0031] (1) Saponification: Take 1 g of the extract in Example 1 into a 50 ml stoppered volumetric flask, add 10 ml of n-hexane and shake gently to melt the oil, and dilute to the mark with n-hexane. Draw 3.00ml of the solution here into another stoppered colorimetric tube, then add 1ml of 2ml / L sodium hydroxide-methanol solution, shake fully for 10min, put it in a water bath at 60°C and heat for 1min~2min, after saponification is completed, cool to room temperature, with methyl esterification;

[0032] (2) Methyl esterification: Add 2ml of 2...

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Abstract

The invention discloses a method for extracting docosahexenoic acid and eicosapentaenoic acid from seriola quinqueradiata. According to the method, a supercritical carbon dioxide fluid extraction technology is adopted; by changing the ratio of an entrainer to raw materials, and the experiment conditions such as experiment temperature, pressure and time, the seriola quinqueradiata rich in docosahexenoic acid and eicosapentaenoic acid is extracted, so as to obtain two unsaturated fatty acids (docosahexenoic acid and eicosapentaenoic acid) which are very important to a human body. The extraction method disclosed by the invention has the characteristics of being high in extraction efficiency, stable in property, convenient, safe, environment-friendly, low in raw material cost, and suitable for preparation of medicines, health products and the like, and has high practical significance.

Description

technical field [0001] The present invention relates to a method for extracting docosahexaenoic acid and eicosapentaenoic acid from yellowtail fish, in particular to a method for extracting docosahexaenoic acid from yellowtail fish using supercritical carbon dioxide fluid extraction technology and eicosapentaenoic acid methods. Background technique [0002] Amberjack, also known as hamachi, is a warm-water fish distributed in the Japan Sea of ​​Japan and the waters south of Taiwan. Yellowtail is rich in DHA, or docosahexaenoic acid. DHA is a highly unsaturated fatty acid essential for human brain nutrition. In addition to preventing the deposition of cholesterol on the blood vessel wall, preventing or reducing the occurrence of atherosclerosis and coronary heart disease, more importantly, DHA has an extremely important effect on brain cells. It accounts for 10% of human brain fat, which is extremely beneficial to the growth and development of brain nerve conduction and sy...

Claims

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Application Information

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IPC IPC(8): C07C51/48C07C57/03
CPCC07C51/48C07C57/03Y02P20/54
Inventor 陈军
Owner 杜丽红
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