Separation medium for purifying proteins, and preparation method thereof
A separation medium and protein technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as fragility, poor strength, and large pressure drop in column operation, and achieve large adsorption capacity and large specific surface area , the effect of high adsorption rate
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Embodiment 1
[0037] A separation medium for purifying proteins, comprising the following raw materials in parts by weight:
[0038] Oil phase:
[0039] Glycidyl methacrylate 40 parts
[0040] 15 parts of ethylene glycol dimethacrylate
[0041] Triallyl isocyanurate 5 parts
[0042] 3 parts divinylbenzene
[0043] 5 parts of hydroxypropyl acrylate
[0044] 50 parts of toluene
[0045] gasoline 10 parts
[0046] 10 parts isoamyl alcohol
[0047] Azobisisobutyronitrile 3 parts
[0048] water box:
[0049] 500 parts of deionized water
[0050] 5 parts polyvinyl alcohol
[0051] 3 parts gelatin
[0052] Sodium chloride 50 parts
[0053] 3 parts methylene blue.
Embodiment 2
[0055] A separation medium for purifying proteins, comprising the following raw materials in parts by weight:
[0056] Oil phase:
[0057] Glycidyl methacrylate 50 parts
[0058] 20 parts of ethylene glycol dimethacrylate
[0059] Triallyl isocyanurate 10 parts
[0060] 5 parts divinylbenzene
[0061] 10 parts of hydroxypropyl acrylate
[0062] 60 parts of toluene
[0063] gasoline 20 parts
[0064] 20 parts of isoamyl alcohol
[0065] Azobisisobutyronitrile 5 parts
[0066] water box:
[0067] 1000 parts of deionized water
[0068] 10 parts polyvinyl alcohol
[0069] 5 parts gelatin
[0070] Sodium chloride 100 parts
[0071] 5 parts methylene blue.
Embodiment 3
[0073] A separation medium for purifying proteins, comprising the following raw materials in parts by weight:
[0074] Oil phase:
[0075] Glycidyl methacrylate 45 parts
[0076] Ethylene glycol dimethacrylate 13 parts
[0077] Triallyl isocyanurate 7.5 parts
[0078] 4 parts divinylbenzene
[0079] 7.5 parts of hydroxypropyl acrylate
[0080] 55 parts of toluene
[0081] gasoline 15 parts
[0082] 15 parts isoamyl alcohol
[0083] Azobisisobutyronitrile 4 parts
[0084] water box:
[0085] 750 parts of deionized water
[0086] 7.5 parts of polyvinyl alcohol
[0087] 4 parts gelatin
[0088] Sodium chloride 75 parts
[0089] 4 parts methylene blue.
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