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A microfluidic chip for micro cell culture

A micro cell and cell culture technology, applied in tissue cell/virus culture devices, animal cells, nervous system cells, etc. question

Inactive Publication Date: 2016-12-07
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fluid flow depletes factors involved in cellular communication
[0005] In the past, the cell culture chip required a large number of cells to be loaded, which could not meet the requirements of micro-cell culture, which limited its application in micro-cell culture.

Method used

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  • A microfluidic chip for micro cell culture
  • A microfluidic chip for micro cell culture
  • A microfluidic chip for micro cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, micro cell culture device

[0056] Such as Figure 1-2 As shown, the micro-cell culture chip of the present invention comprises a cell sampling inlet 1, 8 cell sampling channels 2 and 8 draining channels 5 arranged radially around the cell sampling inlet 1, and the sampling channels 2 There are a number of culture chambers on each side (depending on the density of cultured cells). The culture chambers are connected to the drain channel 5 through the side channel 4. The drain channel 5 is not connected to the cell sampling inlet 1. The end of each drain channel There is a drain outlet 6. The end 8 of the cell sampling channel 2 is closed.

[0057] Among them, the cross-sectional area of ​​the injection inlet 1 is 196250 μm 2 (circular inlet with a diameter of 500 μm); the cross-sectional area of ​​the injection channel 2 is 2500 μm 2 (a square with a length of 50 μm×a width of 50 μm); the volume of the culture chamber 3 is 0.0048 mm 3 ; The cross-secti...

Embodiment 2

[0063] Embodiment 2, cell culture example

[0064] (1) For a density of 1×10 5 cells / mL cell culture medium

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Abstract

The invention relates to a micro fluidic chip for micro cell culture. The cell culture technology is optimized, and a micro fluidic chip for micro cell culture is provided. The micro fluidic chip is easy to manufacture, a micro fluidic device is adopted, and the micro fluidic chip can be used to culture cells massively and efficiently, and can also be applied to micro cell culture, drug sensitive tests, and culture of neuron and stem cells.

Description

technical field [0001] The invention relates to a cell culture container; more specifically, the invention relates to a microfluidic chip for micro-cell culture. Background technique [0002] Microfluidic chips have unique advantages in cell research. For example, DNA and protein analysis can be directly performed on a small number of isolated cells on the chip; the channel diameter of the chip is generally between 10 and 50 μm, which is similar to the size of microvessels and cells in the body, and the perfusion culture method is used to simulate the complex growth of cells in vivo. Microenvironment, so as few as 20-40 cells or even a single cell can be cultured, so it is expected to become an ideal platform for neuron, stem cell culture, rare circulating tumor cells (CTCs) drug sensitivity culture analysis. [0003] Microfabrication and microfluidics provide powerful tools for cell research, including single-cell observation, microenvironment simulation, and high-throughp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12N5/09C12N5/0797
CPCB01L3/5027B01L3/502707B01L3/502715B01L3/502784B01L2200/027B01L2200/10B01L2200/12B01L2300/0864C12M23/16C12N5/0693
Inventor 殷正丰张妤张孝峰
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY