Method for extracting flavones substances in cacumen biotae

A technology of oriental orientalis leaves and flavonoids is applied in the field of extraction of flavonoids to achieve the effects of maintaining activity, saving costs and stabilizing products
CN104523767AActive Publication Date: 2015-04-22南京新图雅生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
南京新图雅生物科技有限公司
Publication Date
2015-04-22

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Abstract

The invention provides a method for extracting flavones substances in cacumen biotae. The method comprises the following steps: cleaning cacumen biotae, drying and crushing the cacumen biotae to obtain cacumen biotae powder; then, adding water to uniformly mix with the cacumen biotae powder to obtain a mixed solution; freezing the mixed solution for 10-24 hours at a temperature of (-)40 DEG C to (-)20 DEG C, and putting the frozen mixed solution at the room temperature until the frozen mixed solution is molten; repeating the freezing step and room-temperature melting step for 2-3 times; adding alcohol to sufficiently mix, performing ultrasonic extraction after soaking for 1-3 hours, centrifugally separating, getting liquid supernatant, and freeze-drying, thereby obtaining a crude product; preparing the crude product into a solution with concentration of 1-3mg / mL, dropwise adding the solution into a macroporous resin column and then performing saturation adsorption, washing and removing with water after the adsorption, taking 60-80% alcohol as an eluent, recycling a solvent from the eluate, concentrating the eluate, and finally performing freeze-drying to obtain flavones substances. The method disclosed by the invention is high in extracting rate, pollution-free and low in cost.
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Description

technical field

[0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids from leaves of oriental cypress. Background technique

[0002] In recent years, due to the development of free radical life sciences, flavonoids with strong antioxidant and free radical elimination effects have received unprecedented attention. Its antioxidant effect is 50 times stronger than VE and 20 times stronger than VC, and it can reach the brain through the blood-brain barrier to prevent and treat diseases of the central nervous system, especially for skin maintenance, rejuvenation and vascular anti-inflammatory effects. At present, there are many reports on extracting flavonoids from Ginkgo biloba leaves, Perilla leaves, Cypress leaves, Sophora japonica flowers, and young leaves of Toona sinensis. However, it is rare to extract flavonoids from the leaves of oriental cypress.

[0003] Arborvitae leaves are the dry shoots and leaves ...

Claims

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