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Method for extracting flavones substances in cacumen biotae

A technology of oriental orientalis leaves and flavonoids is applied in the field of extraction of flavonoids to achieve the effects of maintaining activity, saving costs and stabilizing products

Active Publication Date: 2015-04-22
南京新图雅生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of freeze-thaw technology to extract flavonoids from orientalis leaves

Method used

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  • Method for extracting flavones substances in cacumen biotae
  • Method for extracting flavones substances in cacumen biotae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take fresh arborvitae leaves, weigh them, wash them, and dry them in an oven at 60°C as raw materials; crush them to 40 meshes to obtain arborvitae leaf powder; accurately weigh 20 g of arborvitae leaf powder, add 100 mL of water, Prepare a mixture of cypress leaves; freeze the cypress leaf mixture at -20°C, take it out after 24 hours and thaw it at room temperature, and repeat this three times; add 100 mL of absolute ethanol, mix well, soak for 2 hours, and perform ultrasonic extraction: The ultrasonic power is 60W; the ultrasonic time is 20min; the ultrasonic temperature is 40°C; the supernatant is collected by centrifugal separation. Place the supernatant in a lyophilizer for freeze-drying to obtain the crude product; accurately prepare a solution with a concentration of 1 mg / mL of the crude product, drop it into an AB-8 macroporous resin column for saturated adsorption, and the maximum sample volume is 1.5 times the column volume, first eluted with 5 times the colum...

Embodiment 2

[0034] Accurately weigh 20g of arborvitae leaf powder, add 100mL of water to make a mixture of arborvitae leaves; freeze the mixture of arborvitae leaves at -40°C, take it out after 10h and melt it at room temperature, and repeat this three times; add 100mL of absolute ethanol , fully mixed, after soaking for 2 hours, perform ultrasonic extraction: ultrasonic power 80W; ultrasonic time 40min; ultrasonic temperature 60°C; centrifuge, and take the supernatant. Place the supernatant in a lyophilizer for freeze-drying to obtain the crude product; accurately prepare a solution with a concentration of 2 mg / mL for the crude product, drop it into a D-101 macroporous resin column for saturated adsorption, and the maximum sample volume is 1.5 times the column volume, first eluted with 5 times the column volume of water to remove impurities, then eluted with 5 times the column volume of 70% ethanol, collected the eluate, concentrated, and freeze-dried to obtain a sample of flavonoids from...

Embodiment 3

[0038]Accurately weigh 20g of arborvitae leaf powder, add 100mL of water to make a mixed solution of arborvitae leaves; freeze the mixed solution of arborvitae leaves at -20°C, take it out after 24 hours and thaw at room temperature, and repeat this three times; add 100mL of absolute ethanol , fully mixed, after soaking for 3 hours, perform ultrasonic extraction: ultrasonic power 100W; ultrasonic time 60min; ultrasonic temperature 80°C; centrifuge, and take the supernatant. Place the supernatant in a lyophilizer for freeze-drying to obtain the crude product; accurately prepare a solution with a concentration of 3 mg / mL of the crude product, drop it into a D-130 macroporous resin column for saturated adsorption, and the maximum sample volume is 1.5 times the column volume, first eluted with 5 times the column volume of water to remove impurities, then eluted with 5 times the column volume of 80% ethanol, collected the eluate, concentrated, and freeze-dried to obtain a sample of ...

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Abstract

The invention provides a method for extracting flavones substances in cacumen biotae. The method comprises the following steps: cleaning cacumen biotae, drying and crushing the cacumen biotae to obtain cacumen biotae powder; then, adding water to uniformly mix with the cacumen biotae powder to obtain a mixed solution; freezing the mixed solution for 10-24 hours at a temperature of (-)40 DEG C to (-)20 DEG C, and putting the frozen mixed solution at the room temperature until the frozen mixed solution is molten; repeating the freezing step and room-temperature melting step for 2-3 times; adding alcohol to sufficiently mix, performing ultrasonic extraction after soaking for 1-3 hours, centrifugally separating, getting liquid supernatant, and freeze-drying, thereby obtaining a crude product; preparing the crude product into a solution with concentration of 1-3mg / mL, dropwise adding the solution into a macroporous resin column and then performing saturation adsorption, washing and removing with water after the adsorption, taking 60-80% alcohol as an eluent, recycling a solvent from the eluate, concentrating the eluate, and finally performing freeze-drying to obtain flavones substances. The method disclosed by the invention is high in extracting rate, pollution-free and low in cost.

Description

technical field [0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids from leaves of oriental cypress. Background technique [0002] In recent years, due to the development of free radical life sciences, flavonoids with strong antioxidant and free radical elimination effects have received unprecedented attention. Its antioxidant effect is 50 times stronger than VE and 20 times stronger than VC, and it can reach the brain through the blood-brain barrier to prevent and treat diseases of the central nervous system, especially for skin maintenance, rejuvenation and vascular anti-inflammatory effects. At present, there are many reports on extracting flavonoids from Ginkgo biloba leaves, Perilla leaves, Cypress leaves, Sophora japonica flowers, and young leaves of Toona sinensis. However, it is rare to extract flavonoids from the leaves of oriental cypress. [0003] Arborvitae leaves are the dry shoots and leaves ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/14A61K127/00A61K135/00
CPCA61K36/14A61K2236/15A61K2236/33A61K2236/55
Inventor 都宏霞周玉青许宁胡瑾
Owner 南京新图雅生物科技有限公司
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