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Method for rapidly detecting monosaccharide in tulip tree plant nectar

A technology of Liriodendron chinensis and nectar, applied in the field of chemical analysis, can solve the problems of long analysis time, large sample demand, and complicated methods, and achieve the effect of avoiding interference and reducing detection time

Inactive Publication Date: 2015-04-22
NANJING FORESTRY UNIV
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Problems solved by technology

This method overcomes the disadvantages of complex means, long analysis time and large sample demand of the previous high performance liquid chromatography, and greatly saves the detection cost

Method used

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  • Method for rapidly detecting monosaccharide in tulip tree plant nectar
  • Method for rapidly detecting monosaccharide in tulip tree plant nectar
  • Method for rapidly detecting monosaccharide in tulip tree plant nectar

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Embodiment 1

[0026] Example 1: Detection of glucose and fructose content in nectar of Liriodendron tulipifera and Liriodendron tulipifera flowers by capillary electrophoresis

[0027] (1) Instruments and medicines: capillary electrophoresis instrument (Beckman P / ACE MDQ electrophoresis instrument), UV detector, capillary, 0.45um filter membrane, Liriodendron nectar, Liriodendron nectar (all collected in Jurong City, Jiangsu Province) Liriodendron tulipifera Provenance Test Forest in Xiashu Practice Forest Farm of Nanjing Forestry University in Shu Town), glucose, fructose, Na 2 B 4 o 7 10H 2 O, α-naphthylamine, NaBH3CN, methanol, glacial acetic acid (all reagents are of analytical grade)

[0028] (2) Sample pretreatment: Dilute the fresh nectar of Liriodendron tulipifera and Liriodendron tulipifera by 1 time, filter it with a 0.45um filter membrane, and put the filtrate into a 4°C refrigerator for later use.

[0029] (3) Derivative reagent preparation: Weigh 143.2mg α-naphthylamine and...

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Abstract

The invention discloses a method for rapidly detecting monosaccharide in tulip tree plant nectar and belongs to the field of chemical analysis. Compared with a traditional method for detecting components of plant nectar through high-performance liquid chromatography, the method has the advantages of being rapid, efficient, small in sample size and low in cost. An alpha-naphthylamine derivation method is adopted in the method, a Na2B4O7.10H2O aqueous solution with the pH value being 9.5 serves as a background buffer solution, under the conditions of 20 kv voltage and 0.5 psi of sample feeding conducted for 8 s, glucose and fructose in the nectar can be separated within 20 min, and the content of the glucose and the fructose is measured according to an external standard method.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to using capillary electrophoresis to separate and detect the contents of glucose and fructose in the nectar of Liriodendron genus plants. Background technique [0002] There are two existing species of Liriodendron in Magnoliaceae, one is Liriodendron (L.tulipifera Linn.); the other is Liriodendron [L.chinense (Hemsl.) Sarg.]. Liriodendron genus has bisexual flowers, cup-shaped, open at the same time as the leaves or later; 9 perianth segments, 3 green sepals on the outer ring, spreading outwards; 6 petals on the inner two rings are erect. The flower color of Liriodendron tulipiflora is very different from that of Liriodendron tulipifera. The flowers of Liriodendron tulipifera are yellow-green with yellow vertical stripes near the base, while the flowers of Liriodendron tulipifera are emerald green with orange-yellow or orange-red bands near the base. The nectar secretio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 李火根吕剑南石瑞
Owner NANJING FORESTRY UNIV
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