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31results about How to "Shorten electrophoresis time" patented technology

Chinese medicinal composition for treating hyperlipidemia

The invention discloses a Chinese medicinal composition for treating hyperlipidemia, which relates to a Chinese medicinal composition. The Chinese medicinal composition solves the common problems of more ingredients and unreliable quality caused by complicated manufacturing process in the conventional traditional Chinese medicine for reducing blood fat. The Chinese medicinal composition is prepared from tuber fleeceflower root, hawthorn, wild chrysanthemum, cassia seed, liquoric root, Chinese wolfberry, Chinese yam, oriental waterplantain rhizome, twotooth achyranthes root and red sage root. The Chinese medicinal composition for treating the hyperlipidemia is prepared by mixing traditional Chinese medicines based on traditional Chinese medicine theory; and compared with the conventional Chinese medicinal preparation for treating the hyperlipidemia, the Chinese medicinal composition has fewer traditional Chinese medicine ingredients, is scientific in mixing and prepared from widely available raw materials, does not contain excipients, is not complicatedly manufactured, can be directly taken when crushed and then prepared into capsules, is low in cost, reliable in quality and more suitable for promotion and use, and has no toxic or side effect.
Owner:于庆德

Composition for improving osteoporosis and increasing bone mineral density, preparation method of same and application of the same in preparation of health-caring product

A composition for improving osteoporosis and increasing bone mineral density, a preparation method of the same and an application of the same in preparation of a health-caring product. The composition comprises an active ingredient and auxiliary materials and is characterized in that the active ingredient includes following components, by weight, 65-85 parts of a raw medicine in an extract of epimedium, 5-15 parts of glucosamine, 5-15 parts of chondroitin sulfate, 1.5-3.5 parts of collagen and a proper amount of vitamin D3; and the auxiliary materials include following components, by weight, 1.5-4.5 parts of an excipient and 0.2-1.5 parts of a lubricant. The composition is designed especially for middle-aged and elderly people and is strong in targeted performance. In the invention, the composition is prepared through integration of Chinese and western medicines, not only is traditional Chinese medicine guide theory inherited but also modern medicinal treatment methods are referenced. An experimental result proves that epimedium in the formula can increases the bone mineral density and the chondroitin sulfate, the glucosamine, the collagen and the vitamin D3 have an effect of improving the osteoporosis. The composition has excellent effects of increasing the bone mineral density and promoting absorption of calcium.
Owner:JIANGSU KANGYUAN SUNSHINE PHARMA CO LTD

Drug for treating cardiovascular and cerebrovascular diseases and preparation method thereof

The invention discloses a drug for treating cardiovascular and cerebrovascular diseases and a preparation method thereof. The drug is prepared by danshen root, sanchi, ginkgo leaf, angelica and Astragali radix according to certain weight ratio. The drug of the invention has the efficacy of treating the cardiovascular and cerebrovascular diseases.
Owner:BEIJING INCREASEPHARM CORP LTD

Compound preparation of Chinese herbal medicine for treating chronic prostatitis and its preparing method

A Chinese medicine for treating chronic prostatitis is prepared from 11 Chinese-medicinal materials including leech, safflower, earthworm, astragalus root, etc through proportioning, decocting, collecting decoction, filtering, vacuum concentrating, drying and granulating. Its advantage is high curative effect (86.2% of total effective rate).
Owner:陈子华

Two color-related mutations in apple MdMYB1 gene and detection method thereof

Two color-related mutations in apple MdMYB1 gene and a detection method thereof. The invention belongs to the field of biotechnology and provides a low-cost molecular breeding means for the improvement of apple color traits. A pair of primers is designed by the use of apple color related gene MdMYB1, and apple genome DNA is taken as a template to carry out PCR amplification so as to obtain a PCR amplification segment. Sequence analysis is carried out on PCR amplification segments of 8 apple varieties to find out two gene mutations related with the apple color traits, wherein one gene mutationis Pm1 Iendonuclease site. The enzyme site is used to develop a CAPS marker Mb2P for detecting the mutation. The substantial progress lies in that common agarose gel electrophoresis can be used for the detection of the developed marker so as to greatly reduce experimental cost, in comparison with expensive NuSieve GTG agarose gel electrophoresis which is needed for the detection of the dCAPS marker in the prior art. By the use of the CAPS marker or the sequencing method to detect mutations, apple varieties and the color traits of hybrid posterity fruits can be identified. The color traits of hybrid posterity apples are selected at an early stage to abandon parts of plants, so as to save the management and screening cost of progeny plants.
Owner:SHANDONG INST OF POMOLOGY

Pharmaceutical composition for resisting atherosclerosis and application of pharmaceutical composition

The invention discloses a pharmaceutical composition for resisting atherosclerosis. The pharmaceutical composition is prepared from the following raw materials: astragalus membranaceus, mulberry, rhizoma alismatis, earthworm, scorpio, leech, radix cyathulae, semen cassia, cynanchum paniculatum, long-noded pit viper, salviae miltiorrhizae, radix scrophulariae, honeysuckle, blackend swallowwort root, lucid ganoderma, fructus aurantii, tribulusterrestris, Chinese trumpet creeper, herba cepbalanoplosis segeti, semen sesami nigrum and white peony root. According to the pharmaceutical composition, the living quality of a patient is improved from constitutional symptoms; the patient physique is improved fundamentally; the link of further development of phlegm, deficiency and blood stasis is cut off from the pathogenesis; and the risk of vascular arthralgia telesthesia is reduced from prevention and treatment, so that the pharmaceutical composition is combined with syndrome differentiation and treatment in Chinese medicine and preventive treatment of diseases, and penetrates into the pathogenesis, so that the curative effect is obvious. The pharmaceutical composition is prepared from pure traditional Chinese medicines, so that the pharmaceutical composition is free of a toxic or side effect, easy to accept by the patient, few in raw material components, abundant and available in raw materials, low in price, simple in preparation process and suitable for popularization and application.
Owner:QINGDAO MUNICIPAL HOSPITAL

Novel fruit health care beverage and production process thereof

The invention discloses a novel fruit health care beverage and a production process thereof. The novel fruit health care beverage is characterized by comprising 1000 parts by weight of fruit beverage and 20-100 parts of radix salviae miltiorrhizae; and the production process comprises the following steps of: smashing and soaking the radix salviae miltiorrhizae, extracting juice through a filter press, blending with the fruit beverage, and filtering out impurities and suspended matters to obtain the finished product. The novel fruit health care beverage disclosed by the invention is unique in flavor, good in taste, and rich in nutrition, and can reduce blood pressure, blood lipid and blood sugar by long-term drinking, improve the function of coronary artery, improve the diseases of a central nervous system and realize a certain prevention and treatment effects against coronary heart disease, hypertension, diabetes and insomnia. The preparation method disclosed by the invention is simple in process and low in production cost, and can fully play the health care functions of the ingredients contained in the novel fruit health care beverage.
Owner:DALIAN CHUANGDA TECH TRADE MARKET

Health beverage capable of lowering blood viscosity

The health beverage capable of lowering blood viscosity is prepared with skullcap root 10-30 wt%, motherwort 20-60 wt%, selfheal 15-30 wt%, cassia seed 10-30 wt%, wild chrysanthemum flower 10-30 wt%, haw leaf 3-15 wt%, and tea 5-30 wt%. The health beverage has the health functions of inhibiting platelet aggregation, reducing packed cell volume, lowering blood viscosity, etc and thus can lower theatherosclerosis incidence rate, and prevent hypertension, myocardial infarction, etc.
Owner:白金龙 +1

Red sage root extract sustained-release micropills and preparation method and application thereof

The invention provides red sage root extract sustained-release micropills. Each sustained-release micropill consists of a pill core and a coating film, wherein the pill core comprises a red sage root extract; and the coating film comprises supermicro talcpowder and a copolymer of methyl methacrylate and ethyl acrylate. The invention also provides a method for preparing the red sage root extract sustained release micropills and application of the red sage root extract sustained-release micropills in resisting cardio-cerebrovascular disease. The red sage root extract sustained-release micropills have the advantages of having steady release rate and only needing to be taken twice a day; and the accumulative release percentage reaches 88.4 percent within 12 hours.
Owner:LIVZON PHARM GRP INC +1

Chinese medicine composition for treating lung cancer caused pain

The present invention discloses one kind of Chinese medicine composition, which is prepared with oyster, Dangshen, ophiopogon root, white atractylodes rhizome, date and other Chinese medicinal materials. The Chinese medicine composition is used clinically in light and moderate cancer pain. The present invention also discloses the preparation process of the Chinese medicine composition.
Owner:周小明

Method for rapidly detecting monosaccharide in tulip tree plant nectar

The invention discloses a method for rapidly detecting monosaccharide in tulip tree plant nectar and belongs to the field of chemical analysis. Compared with a traditional method for detecting components of plant nectar through high-performance liquid chromatography, the method has the advantages of being rapid, efficient, small in sample size and low in cost. An alpha-naphthylamine derivation method is adopted in the method, a Na2B4O7.10H2O aqueous solution with the pH value being 9.5 serves as a background buffer solution, under the conditions of 20 kv voltage and 0.5 psi of sample feeding conducted for 8 s, glucose and fructose in the nectar can be separated within 20 min, and the content of the glucose and the fructose is measured according to an external standard method.
Owner:NANJING FORESTRY UNIV

Medicinal composition for treating primary liver cancer

The invention provides a minority traditional Chinese medicinal prescription capable of inhibiting liver cancer, which realizes comprehensive regulation aiming at the pathogenesis of liver cancer. On the basis of the Guangxi Zhuang nationality folk medicine, the inventor studies the traditional Zhuang nationality medicine theory and the traditional Chinese medicine theory, and the long-term clinical application verification shows that the medicinal composition has the good treatment effects. A composition is prepared from the following medicinal materials in parts by weight: 10-15 parts of rhizoma curcumae from Guangxi, 20-30 parts of herba hedyotis diffusae, 15-30 parts of radix corydalis thalictrifoliae, 15-30 parts of fiveleaf gynostemma herb, 6-12 parts of radix bupleuri, 15-30 parts of radix codonopsis, 15-30 parts of semen coicis, and 3-10 parts of leeches.
Owner:广西壮族自治区肿瘤防治研究所

CH4 molecular marker denaturing gel gradient electrophoresis (DGGE) identifying method for aquaculture of catfish hybrids

The invention relates to a CH4 molecular marker DGGE identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, CH4 gene sections in catfishes are selected to serve as molecular markers, the sequence difference the CH4 gene sections between the southern catfishes and the catfishes, denaturing gel gradient electrophoresis detection is performed and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, visual in identification and free from sequencing.
Owner:TONGWEI

Multifunctional constant-speed heating-denatured gradient gel electrophoresis system

The invention relates to a multifunctional constant-speed heating-denatured gradient gel electrophoresis system which is capable of overlapping a gel gradient on a space in a temporal heating process so as to reduce the electrophoresis time and increase the gel resolution. According to the electrophoresis system provided by the invention, constant-speed heating-denatured gradient gel electrophoresis, denatured gradient gel electrophoresis and common polyacrylamide gel electrophoresis can be carried out, and a constant-temperature cold groove is externally connected so as to preform single strand conformation polymorphism, so that one machine has multiple purposes. According to the electrophoresis system, microbial diversity and the relative content of the microbes are quickly and effectively revealed, the functional flora is searched and harmful flora is prevented, and the electrophoresis system has a huge action on industry, agriculture, medicine, environmental monitoring and pollution abatement.
Owner:谭啸

Devices and assembly for collecting biochemical samples

The invention discloses a device and an assembly for collecting biochemical samples, which belong to the technical field of biochemical equipment. The device comprises a collecting and filtering mechanism, a separating and purifying mechanism and a driving mechanism, wherein the collecting and filtering mechanism and the separating and purifying mechanism are removably mounted on the driving mechanism; a through drainage pipe is horizontally connected between the collecting and filtering mechanism and the separating and purifying mechanism; a shunting mechanism of the separating and purifyingmechanism comprises a shunting pipe adopting a Venturi tube principle and resistor loops connected in series with different resistance values; and the driving mechanism can be in butt joint with a first notch and a second notch to drive a first connecting shaft and a second connecting shaft to rotate correspondingly, and is communicated with a first electrode and a second electrode for electrophoretic separation. By adopting the device and the assembly, the phenomena of separation sample belt mixing, sample denaturation and easy capillary tube blockage caused by medium temperature rise due toheating during electrophoresis separation of a biochemical sample are avoided, the buffer solution is recycled to reduce pollution, the separation efficiency is high, the effect is good, the detectionaccuracy can be ensured by storing the samples in vacuum, and all required types of separated samples can be separated and collected at one time.
Owner:WUXI NO 5 PEOPLES HOSPITAL

Method for improving dcaps marker detection efficiency

The invention provides a method for improving dcaps marker detection efficiency, and relates to the technical field of molecular biology. The method for improving the dcaps marker detection efficiencycomprises the following steps: S1, selecting a target gene sequence: extracting a known wheat leaf rust resistance gene Lr67 and a wild type susceptibility gene thereof; S2, designing a dCAPS primer:extracting the wheat leaf rust resistance gene Lr67 in S1 to be matched with dCAPS Finder2.0 equipment to prepare a primer with a normal sequence length; and S3, amplifying the primer sequence length: amplifying the primer prepared in S2. According to the method for improving the dcaps marker detection efficiency, enzyme cutting sites are introduced into primers of dCAPS, and the length of the primers is generally about 20 bp, so that fragments which are about 20 bp shorter than original fragments are generated after PCR enzyme cutting, and polyacrylamide gel electrophoresis is generally adopted for separation so as to distinguish the difference of the fragments of 20 bp. According to the invention, a tail sequence of 30bp or above is added to the 5' end of each primer, so that the fragment size difference between a wild type and a mutant after enzyme digestion is increased to 50bp or above.
Owner:CROP RES INST SHANDONG ACAD OF AGRI SCI

Agarose gel electrophoresis method for detecting microsatellite DNA by using multiple sample application and double-layer electrophoresis

The invention discloses an agarose gel electrophoresis method for detecting microsatellite DNA by using multiple sample application and double-layer electrophoresis, and relates to an agarose gel electrophoresis method for detecting the microsatellite DNA, to solve the problems that agarose powdered sugar and electrophoretic buffer solution are greatly wasted and the EB (Electron Beam) is low in utilization rate during the conventional microsatellite DNA electrophoresis detection. The method comprises the following steps of: putting two pieces of sepharose gel of the same areas on two up and down layers in parallel; carrying out sample application and electrophoresis on the lower layer and the upper layer in sequence till detected amplification products reach the bottom of the gel, stopping the electrophoresis; and subsequently carrying out gel imaging detection. By utilizing the method, the utilization rate of the sepharose gel and the electrophoresis buffer solution is greatly improved, and the labor and the cost are saved; compared with one-time sample application, no gel needs to be reprepared, one piece of 10-cm gel can be repeated for sample application for 8 times, so that the cost can be saved by more than 8 times. Therefore, rapid and efficient detection plant SSR (Solid State Relay) marker-site heritable variation is realized.
Owner:QIQIHAR UNIVERSITY

DNA rapid identification method for ivories and products thereof

The invention provides a DNA rapid identification method for ivories and products thereof. By comparing different decalcification treatment, an ultrasonic treatment method (ultrasonic treatment for 2hat 37 DEG C) with a good decalcification effect and short consumed time is screened out. Extracted DNA is amplified with an SYBR Green real-time fluorescent polymerase chain reaction (PCR) method instead of a common PCR method, and without a subsequent PCR product electrophoresis process, sequence determination is directly performed on a real-time fluorescent PCR product with an amplification curve and a melting curve and of which the melting temperature is 82.00 DEG C+ / -0.5 DEG C, so that experimental procedures and laboratory pollution are reduced; and ultrasonic decalcification and the SYBR Green real-time fluorescent PCR method are combined, and thus, the experimental time is greatly shortened, and the species of the ivories and the products thereof can be quickly identified.
Owner:INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Devices and assemblies for collection of biochemical samples

The invention discloses a device and an assembly for collecting biochemical samples, belonging to the technical field of biochemical equipment, comprising a collecting and filtering mechanism, a separation and purification mechanism and a driving mechanism; There is a through drainage tube horizontally connected between the mechanism and the separation and purification mechanism; the shunt mechanism of the separation and purification mechanism includes a shunt tube using the Venturi tube principle and a resistance circuit with different resistance values ​​connected in series; the driving mechanism can be connected to the first slot and the second slot. The connecting shaft 1 and the connecting shaft 2 are respectively driven to rotate, and the electrode 1 and the electrode 2 are connected for electrophoretic separation. It solves the phenomenon that the separation sample is mixed, the sample is denatured and the capillary is easy to block due to the heating of the medium temperature caused by the electrophoretic separation of the biochemical sample. The buffer solution is recycled to reduce pollution, the separation efficiency is high, the effect is good, and the vacuum preservation of the sample can ensure the accuracy of detection. It is able to separate and collect all desired types of separated samples at one time.
Owner:WUXI NO 5 PEOPLES HOSPITAL

A kind of anti-atherosclerosis pharmaceutical composition and its application

The invention discloses a pharmaceutical composition for resisting atherosclerosis. The pharmaceutical composition is prepared from the following raw materials: astragalus membranaceus, mulberry, rhizoma alismatis, earthworm, scorpio, leech, radix cyathulae, semen cassia, cynanchum paniculatum, long-noded pit viper, salviae miltiorrhizae, radix scrophulariae, honeysuckle, blackend swallowwort root, lucid ganoderma, fructus aurantii, tribulusterrestris, Chinese trumpet creeper, herba cepbalanoplosis segeti, semen sesami nigrum and white peony root. According to the pharmaceutical composition, the living quality of a patient is improved from constitutional symptoms; the patient physique is improved fundamentally; the link of further development of phlegm, deficiency and blood stasis is cut off from the pathogenesis; and the risk of vascular arthralgia telesthesia is reduced from prevention and treatment, so that the pharmaceutical composition is combined with syndrome differentiation and treatment in Chinese medicine and preventive treatment of diseases, and penetrates into the pathogenesis, so that the curative effect is obvious. The pharmaceutical composition is prepared from pure traditional Chinese medicines, so that the pharmaceutical composition is free of a toxic or side effect, easy to accept by the patient, few in raw material components, abundant and available in raw materials, low in price, simple in preparation process and suitable for popularization and application.
Owner:QINGDAO MUNICIPAL HOSPITAL

A rapid dna identification method for ivory and its products

The invention provides a DNA rapid identification method for ivory and its products. By comparing different decalcification treatments, the ultrasonic treatment method with good decalcification effect and short time consumption was selected (37°C ultrasonic treatment for 2 h). Use the SYBR Green real-time fluorescent PCR method instead of the ordinary PCR method to amplify the extracted DNA, without the need for subsequent electrophoresis of PCR products, and directly generate real-time fluorescent PCR with amplification curves and melting curves and a melting temperature of 82.00°C±0.5°C The product is sequenced, reducing experimental steps and laboratory pollution. The combination of ultrasonic decalcification and SYBR Green real-time fluorescent PCR method greatly shortens the experimental time and can quickly identify the species of ivory and its products.
Owner:INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Drug for treating cardiovascular and cerebrovascular diseases and preparation method thereof

The invention discloses a drug for treating cardiovascular and cerebrovascular diseases and a preparation method thereof. The drug is prepared by danshen root, sanchi, ginkgo leaf, angelica and Astragali radix according to certain weight ratio. The drug of the invention has the efficacy of treating the cardiovascular and cerebrovascular diseases.
Owner:BEIJING INCREASEPHARM CORP LTD

Traditional Chinese medicine used for preventing and treating cerebral thrombosis and preparation method thereof

The invention discloses a traditional Chinese medicine used for preventing and treating cerebral thrombosis and a preparation method thereof. The traditional Chinese medicine used for preventing and treating cerebral thrombosis is prepared from the following components in mass: 6-9g of ligusticum wallichii, 10-15g of salviae miltiorrhizae, 5-10g of safflower, 8-10g of peach kernel, 5-10g of dried body of ground beetle and 8-10g of artemisia anomala. The preparation method comprises the following steps of: cleaning and soaking raw materials, boiling medicine for four times, and converging medicine juices obtained by boiling, thus the traditional Chinese medicine which is used for preventing and treating cerebral thrombosis and can be taken is obtained. According to the traditional Chinese medicine used for preventing and treating cerebral thrombosis, components are combined, functions and effects of the components are fully played, and the traditional Chinese medicine used for preventing and treating cerebral thrombosis has the effects of promoting blood circulation to remove meridian obstruction, eliminating abdominal mass and diluting thick blood, so that blood circulation is smooth, symptoms such as numbness of limbs, inconvenience in action, numbness of mouth and tongue and blurred vision are eliminated, and the traditional Chinese medicine used for preventing and treating cerebral thrombosis has a good effect for preventing and treating cerebral thrombosis.
Owner:宫恩淇

Method for SSR (simple sequence repeat) analysis of tetraploid alfalfa by multiplex PCR (polymerase chain reaction) fluorescence labeling technique

The invention relates to a method for SSR (simple sequence repeat) analysis of tetraploid alfalfa by a multiplex PCR (polymerase chain reaction) fluorescence labeling technique. The method involves nine multiplex PCR groups (including 23 pairs of SSR primers and a universal primer) and corresponding PCR reaction systems and reaction programs. Amplification product separation is realized by conventional denatured polyacrylamide gel electrophoresis, a gradient multifunctional laser scanning imager (Amersham Biosciences, USA) is adopted for scanning and recording images under different wave lengths. 23 pairs of SSR labels are uniformly distributed on chromosomes and a label interval is not smaller than 10Mbp, labeled genotypes are easily recognizable, and the average genotype accuracy rate ishigh than 95%. By adoption of the scheme for genetic diversity analysis, group structure analysis and variety identification of tetraploid alfalfa, short time and high accuracy are realized, and testefficiency is remarkably improved.
Owner:TIANJIN AGRICULTURE COLLEGE

Traditional Chinese medicine composition with analgesic effect and preparation method of traditional Chinese medicine composition

The invention discloses a traditional Chinese medicine composition with an analgesic effect and a preparation method of the traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine. The traditional Chinese medicine composition is prepared by adopting ways of water decoction, steam distillation and the like to extract active ingredients from bulk drugs such as Corydalis yanhusuo, Radix Paeoniae Rubra, Herba Asari, Venenum Bufonis, Xanthium sibiricum fruit, Glycyrriza uralensis and the like according to different properties of the drugs, and prepare into capsules, tablets, granulas and pills. The traditional Chinese medicine composition provided by the invention has the effects of promoting qi and activating blood, warming and activating meridian, clearing away heat and removing toxicity, and inducing resuscitation, and is mainly used for curing mild and moderate cancer pain caused by a lung cancer, a gastric cancer, a liver cancer and the like.
Owner:JILIN BAINIAN HANKE PHARM CO LTD
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