31results about How to "Shorten electrophoresis time" patented technology

Two color-related mutations in apple MdMYB1 gene and a detection method thereof. The invention belongs to the field of biotechnology and provides a low-cost molecular breeding means for the improvement of apple color traits. A pair of primers is designed by the use of apple color related gene MdMYB1, and apple genome DNA is taken as a template to carry out PCR amplification so as to obtain a PCR amplification segment. Sequence analysis is carried out on PCR amplification segments of 8 apple varieties to find out two gene mutations related with the apple color traits, wherein one gene mutationis Pm1 Iendonuclease site. The enzyme site is used to develop a CAPS marker Mb2P for detecting the mutation. The substantial progress lies in that common agarose gel electrophoresis can be used for the detection of the developed marker so as to greatly reduce experimental cost, in comparison with expensive NuSieve GTG agarose gel electrophoresis which is needed for the detection of the dCAPS marker in the prior art. By the use of the CAPS marker or the sequencing method to detect mutations, apple varieties and the color traits of hybrid posterity fruits can be identified. The color traits of hybrid posterity apples are selected at an early stage to abandon parts of plants, so as to save the management and screening cost of progeny plants.

The health beverage capable of lowering blood viscosity is prepared with skullcap root 10-30 wt%, motherwort 20-60 wt%, selfheal 15-30 wt%, cassia seed 10-30 wt%, wild chrysanthemum flower 10-30 wt%, haw leaf 3-15 wt%, and tea 5-30 wt%. The health beverage has the health functions of inhibiting platelet aggregation, reducing packed cell volume, lowering blood viscosity, etc and thus can lower theatherosclerosis incidence rate, and prevent hypertension, myocardial infarction, etc.

The invention discloses a device and an assembly for collecting biochemical samples, which belong to the technical field of biochemical equipment. The device comprises a collecting and filtering mechanism, a separating and purifying mechanism and a driving mechanism, wherein the collecting and filtering mechanism and the separating and purifying mechanism are removably mounted on the driving mechanism; a through drainage pipe is horizontally connected between the collecting and filtering mechanism and the separating and purifying mechanism; a shunting mechanism of the separating and purifyingmechanism comprises a shunting pipe adopting a Venturi tube principle and resistor loops connected in series with different resistance values; and the driving mechanism can be in butt joint with a first notch and a second notch to drive a first connecting shaft and a second connecting shaft to rotate correspondingly, and is communicated with a first electrode and a second electrode for electrophoretic separation. By adopting the device and the assembly, the phenomena of separation sample belt mixing, sample denaturation and easy capillary tube blockage caused by medium temperature rise due toheating during electrophoresis separation of a biochemical sample are avoided, the buffer solution is recycled to reduce pollution, the separation efficiency is high, the effect is good, the detectionaccuracy can be ensured by storing the samples in vacuum, and all required types of separated samples can be separated and collected at one time.

The health beverage capable of lowering blood viscosity is prepared with skullcap root 10-30 wt%, motherwort 20-60 wt%, selfheal 15-30 wt%, cassia seed 10-30 wt%, wild chrysanthemum flower 10-30 wt%, haw leaf 3-15 wt%, and tea 5-30 wt%. The health beverage has the health functions of inhibiting platelet aggregation, reducing packed cell volume, lowering blood viscosity, etc and thus can lower theatherosclerosis incidence rate, and prevent hypertension, myocardial infarction, etc.

The invention discloses a traditional Chinese medicine composition with an analgesic effect and a preparation method of the traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine. The traditional Chinese medicine composition is prepared by adopting ways of water decoction, steam distillation and the like to extract active ingredients from bulk drugs such as Corydalis yanhusuo, Radix Paeoniae Rubra, Herba Asari, Venenum Bufonis, Xanthium sibiricum fruit, Glycyrriza uralensis and the like according to different properties of the drugs, and prepare into capsules, tablets, granulas and pills. The traditional Chinese medicine composition provided by the invention has the effects of promoting qi and activating blood, warming and activating meridian, clearing away heat and removing toxicity, and inducing resuscitation, and is mainly used for curing mild and moderate cancer pain caused by a lung cancer, a gastric cancer, a liver cancer and the like.

The invention discloses an agarose gel electrophoresis method for detecting microsatellite DNA by using multiple sample application and double-layer electrophoresis, and relates to an agarose gel electrophoresis method for detecting the microsatellite DNA, to solve the problems that agarose powdered sugar and electrophoretic buffer solution are greatly wasted and the EB (Electron Beam) is low in utilization rate during the conventional microsatellite DNA electrophoresis detection. The method comprises the following steps of: putting two pieces of sepharose gel of the same areas on two up and down layers in parallel; carrying out sample application and electrophoresis on the lower layer and the upper layer in sequence till detected amplification products reach the bottom of the gel, stopping the electrophoresis; and subsequently carrying out gel imaging detection. By utilizing the method, the utilization rate of the sepharose gel and the electrophoresis buffer solution is greatly improved, and the labor and the cost are saved; compared with one-time sample application, no gel needs to be reprepared, one piece of 10-cm gel can be repeated for sample application for 8 times, so that the cost can be saved by more than 8 times. Therefore, rapid and efficient detection plant SSR (Solid State Relay) marker-site heritable variation is realized.

The invention discloses a Chinese medicinal composition for treating hyperlipidemia, which relates to a Chinese medicinal composition. The Chinese medicinal composition solves the common problems of more ingredients and unreliable quality caused by complicated manufacturing process in the conventional traditional Chinese medicine for reducing blood fat. The Chinese medicinal composition is prepared from tuber fleeceflower root, hawthorn, wild chrysanthemum, cassia seed, liquoric root, Chinese wolfberry, Chinese yam, oriental waterplantain rhizome, twotooth achyranthes root and red sage root. The Chinese medicinal composition for treating the hyperlipidemia is prepared by mixing traditional Chinese medicines based on traditional Chinese medicine theory; and compared with the conventional Chinese medicinal preparation for treating the hyperlipidemia, the Chinese medicinal composition has fewer traditional Chinese medicine ingredients, is scientific in mixing and prepared from widely available raw materials, does not contain excipients, is not complicatedly manufactured, can be directly taken when crushed and then prepared into capsules, is low in cost, reliable in quality and more suitable for promotion and use, and has no toxic or side effect.

A Chinese medicine for treating chronic prostatitis is prepared from 11 Chinese-medicinal materials including leech, safflower, earthworm, astragalus root, etc through proportioning, decocting, collecting decoction, filtering, vacuum concentrating, drying and granulating. Its advantage is high curative effect (86.2% of total effective rate).

The invention discloses a device and an assembly for collecting biochemical samples, belonging to the technical field of biochemical equipment, comprising a collecting and filtering mechanism, a separation and purification mechanism and a driving mechanism; There is a through drainage tube horizontally connected between the mechanism and the separation and purification mechanism; the shunt mechanism of the separation and purification mechanism includes a shunt tube using the Venturi tube principle and a resistance circuit with different resistance values ​​connected in series; the driving mechanism can be connected to the first slot and the second slot. The connecting shaft 1 and the connecting shaft 2 are respectively driven to rotate, and the electrode 1 and the electrode 2 are connected for electrophoretic separation. It solves the phenomenon that the separation sample is mixed, the sample is denatured and the capillary is easy to block due to the heating of the medium temperature caused by the electrophoretic separation of the biochemical sample. The buffer solution is recycled to reduce pollution, the separation efficiency is high, the effect is good, and the vacuum preservation of the sample can ensure the accuracy of detection. It is able to separate and collect all desired types of separated samples at one time.

The invention discloses a pharmaceutical composition for resisting atherosclerosis. The pharmaceutical composition is prepared from the following raw materials: astragalus membranaceus, mulberry, rhizoma alismatis, earthworm, scorpio, leech, radix cyathulae, semen cassia, cynanchum paniculatum, long-noded pit viper, salviae miltiorrhizae, radix scrophulariae, honeysuckle, blackend swallowwort root, lucid ganoderma, fructus aurantii, tribulusterrestris, Chinese trumpet creeper, herba cepbalanoplosis segeti, semen sesami nigrum and white peony root. According to the pharmaceutical composition, the living quality of a patient is improved from constitutional symptoms; the patient physique is improved fundamentally; the link of further development of phlegm, deficiency and blood stasis is cut off from the pathogenesis; and the risk of vascular arthralgia telesthesia is reduced from prevention and treatment, so that the pharmaceutical composition is combined with syndrome differentiation and treatment in Chinese medicine and preventive treatment of diseases, and penetrates into the pathogenesis, so that the curative effect is obvious. The pharmaceutical composition is prepared from pure traditional Chinese medicines, so that the pharmaceutical composition is free of a toxic or side effect, easy to accept by the patient, few in raw material components, abundant and available in raw materials, low in price, simple in preparation process and suitable for popularization and application.

The invention relates to a CH4 molecular marker DGGE identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, CH4 gene sections in catfishes are selected to serve as molecular markers, the sequence difference the CH4 gene sections between the southern catfishes and the catfishes, denaturing gel gradient electrophoresis detection is performed and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, visual in identification and free from sequencing.

The invention provides a DNA rapid identification method for ivory and its products. By comparing different decalcification treatments, the ultrasonic treatment method with good decalcification effect and short time consumption was selected (37°C ultrasonic treatment for 2 h). Use the SYBR Green real-time fluorescent PCR method instead of the ordinary PCR method to amplify the extracted DNA, without the need for subsequent electrophoresis of PCR products, and directly generate real-time fluorescent PCR with amplification curves and melting curves and a melting temperature of 82.00°C±0.5°C The product is sequenced, reducing experimental steps and laboratory pollution. The combination of ultrasonic decalcification and SYBR Green real-time fluorescent PCR method greatly shortens the experimental time and can quickly identify the species of ivory and its products.

The invention discloses a drug for treating cardiovascular and cerebrovascular diseases and a preparation method thereof. The drug is prepared by danshen root, sanchi, ginkgo leaf, angelica and Astragali radix according to certain weight ratio. The drug of the invention has the efficacy of treating the cardiovascular and cerebrovascular diseases.

The invention discloses a traditional Chinese medicine used for preventing and treating cerebral thrombosis and a preparation method thereof. The traditional Chinese medicine used for preventing and treating cerebral thrombosis is prepared from the following components in mass: 6-9g of ligusticum wallichii, 10-15g of salviae miltiorrhizae, 5-10g of safflower, 8-10g of peach kernel, 5-10g of dried body of ground beetle and 8-10g of artemisia anomala. The preparation method comprises the following steps of: cleaning and soaking raw materials, boiling medicine for four times, and converging medicine juices obtained by boiling, thus the traditional Chinese medicine which is used for preventing and treating cerebral thrombosis and can be taken is obtained. According to the traditional Chinese medicine used for preventing and treating cerebral thrombosis, components are combined, functions and effects of the components are fully played, and the traditional Chinese medicine used for preventing and treating cerebral thrombosis has the effects of promoting blood circulation to remove meridian obstruction, eliminating abdominal mass and diluting thick blood, so that blood circulation is smooth, symptoms such as numbness of limbs, inconvenience in action, numbness of mouth and tongue and blurred vision are eliminated, and the traditional Chinese medicine used for preventing and treating cerebral thrombosis has a good effect for preventing and treating cerebral thrombosis.