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A rapid dna identification method for ivory and its products

An identification method and product technology, applied in the biological field, to achieve the effects of improving accuracy, avoiding pollution, and saving electrophoresis time

Active Publication Date: 2022-05-27
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

precipitationfor DNA extraction

Method used

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  • A rapid dna identification method for ivory and its products
  • A rapid dna identification method for ivory and its products
  • A rapid dna identification method for ivory and its products

Examples

Experimental program
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Embodiment Construction

[0027] In order to make the content of the present invention more understandable, the following technical solutions described in conjunction with specific embodiments of the present invention will be further described, but the following examples are only examples of the present invention, and do not represent the scope of rights protection limited by the present invention, the scope of rights protection of the present invention is subject to the claims.

[0028] Real-time example 1

[0029] 1 Test material

[0030] Ivory and its products are collected by this laboratory.

[0031] 2 Main reagents

[0032]The lysate [5mol / L guanidine isothiocyanate, 0.05 mol / L Tris-HCl (pH6.4), 0.02 mol / L EDTA (pH8.0), 1.3% Triton X-100 (v / v)] was formulated in-house; SYBR Premix Ex Taq (2×) was produced by Bao Bioengineering (Dalian) Co., Ltd. (Dalian TaKaRa Company).

[0033] 3 Main instruments and equipment

[0034] Hybrid grinder, vortex meter, water bath pot, ultrasonic instrument, tabletop cen...

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Abstract

The invention provides a DNA rapid identification method for ivory and its products. By comparing different decalcification treatments, the ultrasonic treatment method with good decalcification effect and short time consumption was selected (37°C ultrasonic treatment for 2 h). Use the SYBR Green real-time fluorescent PCR method instead of the ordinary PCR method to amplify the extracted DNA, without the need for subsequent electrophoresis of PCR products, and directly generate real-time fluorescent PCR with amplification curves and melting curves and a melting temperature of 82.00°C±0.5°C The product is sequenced, reducing experimental steps and laboratory pollution. The combination of ultrasonic decalcification and SYBR Green real-time fluorescent PCR method greatly shortens the experimental time and can quickly identify the species of ivory and its products.

Description

Technical field [0001] The present invention relates to a method of rapid identification of DNA of ivory and its products, belonging to the field of biotechnology. Background [0002] Ivory smuggling is becoming increasingly rampant, and in order to effectively combat ivory smuggling and protect wild endangered animals, ivory species identification technology is urgently needed. The main types of ivory include: mammoth ( Mammuthus primigenius ) tooth, Asian elephant ( Elephas maximus Linnaeus) Tooth, African elephant ( Loxodonta africana Teeth, of which mammoth tusks are fossils, Asian elephants and African elephants are endangered species. After the three kinds of ivory are processed into various tourism products or works of art, it is difficult to distinguish their species from the morphology, but molecular biology methods can be used to accurately identify the species of ivory. DNA extraction is a key link in molecular biology identification methods, but due to the high calci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/10C12Q1/6806C12Q1/686C12N15/11
CPCC12Q1/6888C12N15/1003C12Q1/686C12Q1/6806C12Q2561/113C12Q2563/107
Inventor 邵碧英陈文炳彭娟缪婷玉
Owner INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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